AB214214

Anti-IDO-2 antibody

Name: Anti-IDO-2 antibody (ab214214) | Abcam
Brand: Abcam Limited
SKU: AB214214

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(4 Publications )

Rabbit Polyclonal IDO-2 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human IDO2 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

INDOL1, IDO2, IDO-2

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDO-2 antibody (AB214214)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDO-2 antibody (AB214214)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling IDO-2 with ab214214 at 1/500 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human IDO2 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Indoleamine 23-dioxygenase 2 (IDO-2) is an enzyme involved in tryptophan catabolism. IDO-2 is also known as INDOL1 or INDOLE-2. This enzyme differs from its counterpart IDO1 in its substrate specificity and tissue distribution. IDO-2 has a molecular mass of approximately 45 kDa. It is expressed in various tissues including liver kidney and placenta with notably higher expression in immune system cells like dendritic cells and macrophages.

Biological function summary

IDO-2 participates in the degradation of the essential amino acid tryptophan via the kynurenine pathway. This process helps modulate immune responses by reducing tryptophan availability and producing metabolites that affect T-cell function. IDO-2 is not known to be part of a larger protein complex but it does work in parallel with IDO1 to contribute to immune tolerance and homeostasis within the body.

Pathways

The kynurenine pathway prominently involves IDO-2 where it aids in the conversion of tryptophan to kynurenine influencing pathways related to immune regulation. IDO-2 interacts closely with enzymes such as tryptophan 23-dioxygenase (TDO) sharing roles in tryptophan metabolism. Both IDO2 and IDO1 can lead to the production of kynurenine highlighting their shared influence within this metabolic pathway.

The activity of IDO-2 plays a role in conditions like cancer and autoimmune diseases. In cancer its ability to suppress immune attack on tumors by depleting tryptophan and generating immunosuppressive metabolites allows tumor cells to escape immune surveillance. In autoimmune disorders like systemic lupus erythematosus altered tryptophan metabolism through IDO-2 involvement can affect disease progression. IDO-2 works alongside IDO1 within these pathological contexts influencing disease outcomes due to their shared enzymatic roles in immune modulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed : 17671174). Involved in immune regulation. May not play a significant role in tryptophan-related tumoral resistance (PubMed : 25691885).

See full target information IDO2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 27: PubMed36484383

2022

Absence of indoleamine 2,3‑dioxygenase 2 promotes liver regeneration after partial hepatectomy in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuya Ando,Masato Hoshi,Hiroyuki Tezuka,Hiroyasu Ito,Kentaro Nakamoto,Yasuko Yamamoto,Kuniaki Saito

International journal of molecular sciences 21: PubMed32752186

2020

Deficiency Exacerbates Imiquimod-Induced Psoriasis-Like Skin Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Kento Fujii,Yasuko Yamamoto,Yoko Mizutani,Kuniaki Saito,Mariko Seishima

Cancer science 110:3061-3067 PubMed31444833

2019

Indoleamine 2,3-dioxygenase 2 depletion suppresses tumor growth in a mouse model of Lewis lung carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wakana Yamasuge,Yasuko Yamamoto,Hidetsugu Fujigaki,Masato Hoshi,Kentaro Nakamoto,Kazuo Kunisawa,Akihiro Mouri,Toshitaka Nabeshima,Kuniaki Saito

Scientific reports 8:15917 PubMed30374077

2018

Lipopolysaccharide shock reveals the immune function of indoleamine 2,3-dioxygenase 2 through the regulation of IL-6/stat3 signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuko Yamamoto,Wakana Yamasuge,Shinjiro Imai,Kazuo Kunisawa,Masato Hoshi,Hidetsugu Fujigaki,Akihiro Mouri,Toshitaka Nabeshima,Kuniaki Saito

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IDO-2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDO-2 antibody (AB214214)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise