AB278320

Anti-IDO-2 antibody [EPR23690A-48] - BSA and Azide free (Capture)

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal IDO-2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

INDOL1, IDO2, IDO-2

1 Images

Sandwich ELISA - Anti-IDO-2 antibody [EPR23690A-48] - BSA and Azide free (Capture) (AB278320)

sELISA

Lab

Sandwich ELISA - Anti-IDO-2 antibody [EPR23690A-48] - BSA and Azide free (Capture) (AB278320)

Sandwich ELISA of ab253570 with the capture antibody ab278320 dilution at 2 µg/mL and detector antibody ab278322 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23690A-48

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

style

left-column

Product details

ab278320 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human IDO2. The recommended pair for sandwich ELISA is:
Capture: ab278320, Human IDO2 Capture Antibody (unconjugated)
Detector: ab278322, Human IDO2 Detector Antibody (unconjugated)
The reference range value is 78.13 - 5000 pg/mL.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Indoleamine 23-dioxygenase 2 (IDO-2) is an enzyme involved in tryptophan catabolism. IDO-2 is also known as INDOL1 or INDOLE-2. This enzyme differs from its counterpart IDO1 in its substrate specificity and tissue distribution. IDO-2 has a molecular mass of approximately 45 kDa. It is expressed in various tissues including liver kidney and placenta with notably higher expression in immune system cells like dendritic cells and macrophages.

Biological function summary

IDO-2 participates in the degradation of the essential amino acid tryptophan via the kynurenine pathway. This process helps modulate immune responses by reducing tryptophan availability and producing metabolites that affect T-cell function. IDO-2 is not known to be part of a larger protein complex but it does work in parallel with IDO1 to contribute to immune tolerance and homeostasis within the body.

Pathways

The kynurenine pathway prominently involves IDO-2 where it aids in the conversion of tryptophan to kynurenine influencing pathways related to immune regulation. IDO-2 interacts closely with enzymes such as tryptophan 23-dioxygenase (TDO) sharing roles in tryptophan metabolism. Both IDO2 and IDO1 can lead to the production of kynurenine highlighting their shared influence within this metabolic pathway.

The activity of IDO-2 plays a role in conditions like cancer and autoimmune diseases. In cancer its ability to suppress immune attack on tumors by depleting tryptophan and generating immunosuppressive metabolites allows tumor cells to escape immune surveillance. In autoimmune disorders like systemic lupus erythematosus altered tryptophan metabolism through IDO-2 involvement can affect disease progression. IDO-2 works alongside IDO1 within these pathological contexts influencing disease outcomes due to their shared enzymatic roles in immune modulation.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed : 17671174). Involved in immune regulation. May not play a significant role in tryptophan-related tumoral resistance (PubMed : 25691885).

See full target information IDO2

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IDO-2 antibody [EPR23690A-48] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-IDO-2 antibody [EPR23690A-48] - BSA and Azide free (Capture) (AB278320)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise