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AB74562

Anti-IDOL antibody

4

(4 Reviews)

|

(14 Publications)

Rabbit Polyclonal IDOL antibody. Suitable for ELISA, WB, IHC-P and reacts with Mouse, Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human MYLIP.

View Alternative Names

BZF1, IDOL, BM-023, PP5242, MYLIP, E3 ubiquitin-protein ligase MYLIP, Inducible degrader of the LDL-receptor, Myosin regulatory light chain interacting protein, RING-type E3 ubiquitin transferase MYLIP, Idol, MIR

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDOL antibody (AB74562)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDOL antibody (AB74562)

Immunohistochemistry analysis of IDOL in paraffin embedded human brain tissue using 1/50 ab74562.
Left image : untreated sample.
Right image : sample treated with immunising peptide.

Western blot - Anti-IDOL antibody (AB74562)
  • WB

Unknown

Western blot - Anti-IDOL antibody (AB74562)

All lanes:

Western blot - Anti-IDOL antibody (ab74562) at 1/500 dilution

Lane 1:

extracts from HeLa cells at 10 µg

Lane 2:

extracts from Jurkat cells at 10 µg

Lane 3:

extracts from Jurkat cells at 10 µg with immunising peptide

Secondary

All lanes:

Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution

Predicted band size: 49 kDa

Observed band size: 35 kDa,50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

IHC-P, WB, ELISA

applications

Immunogen

Synthetic Peptide within Human MYLIP. The exact immunogen used to generate this antibody is proprietary information.

Q8WY64

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Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

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Primary Antibodies

AB18256

Anti-HMGB1 antibody

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Western blot - Anti-HMGB1 antibody (AB18256)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IDOL known as Inducible Degrader of the Low-density lipoprotein receptor plays an important role in protein degradation especially the degradation of the LDL receptor (LDLR). The IDOL protein also referred to as MYLIP (Myosin Regulatory Light Chain Interacting Protein) has a molecular mass of approximately 54 kDa. It is widely expressed in tissues with notable expression in the brain liver and macrophages. IDOL functions as an E3 ubiquitin ligase tagging LDLR for degradation which subsequently influences cholesterol levels in the body.
Biological function summary

Within cellular systems IDOL regulates cholesterol homeostasis by controlling the degradation of LDL receptors. It is not directly part of a large protein complex but interacts with other proteins involved in lipid metabolism. By mediating LDLR degradation IDOL indirectly affects cholesterol uptake by cells. IDOL's regulatory mechanism is vital in preventing excessive cellular cholesterol accumulation which can lead to various metabolic problems if unregulated.

Pathways

IDOL serves an important role in the lipid metabolic pathways and is part of the broader cholesterol efflux regulatory network. IDOL acts downstream of the Liver X Receptor (LXR) pathway. The LXR upon activation by oxysterols promotes IDOL expression leading to enhanced LDL receptor degradation. This pathway makes IDOL an important regulator of cholesterol levels linking it with proteins like LDLR and the LXRs. In this regulatory network IDOL ensures balanced cholesterol levels by modulating receptor availability and cholesterol uptake in the liver and peripheral tissues.

IDOL is implicated in conditions such as hypercholesterolemia and atherosclerosis due to its cholesterol regulatory functions. Alterations in IDOL activity or expression can lead to abnormal cholesterol buildup raising the risk of cardiovascular diseases. The connection between IDOL and LDLR is critical as both proteins influence cholesterol metabolism disorders. Mutations or dysregulation affecting IDOL can disturb normal lipid homeostasis consequently contributing to disease progression. Understanding IDOL’s mechanism further opens avenues for potential therapeutic interventions targeting cholesterol-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
See full target information MYLIP
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 55: PubMed40116083

2025

ABCG8‑mediated sterol efflux increases cancer cell progression via the LRP6/Wnt/β‑catenin signaling pathway in radiotherapy‑resistant MDA‑MB‑231 triple‑negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Young Shin Ko,Ju Yeong Won,Hana Jin,Nam Binh Nguyen,Yaeram Won,Vedaste Nsanzimana,Seung Pil Yun,Sang Won Park,Hye Jung Kim

Journal of translational medicine 22:352 PubMed38622667

2024

Quinic acid regulated TMA/TMAO-related lipid metabolism and vascular endothelial function through gut microbiota to inhibit atherosclerotic.

Applications

Unspecified application

Species

Unspecified reactive species

Qiao Jin,Chiyuan Zhang,Ran Chen,Luping Jiang,Hongli Li,Pengcui Wu,Liang Li

Antioxidants (Basel, Switzerland) 12: PubMed36830035

2023

Di-(2-ethylhexyl) Phthalate Limits the Lipid-Lowering Effects of Simvastatin by Promoting Protein Degradation of Low-Density Lipoprotein Receptor: Role of PPARγ-PCSK9 and LXRα-IDOL Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Bei-Chia Guo,Ko-Lin Kuo,Jenq-Wen Huang,Chia-Hui Chen,Der-Cherng Tarng,Tzong-Shyuan Lee

Endocrine connections 11: PubMed35560019

2022

Effects of statins on the inducible degrader of low-density lipoprotein receptor in familial hypercholesterolemia.

Applications

Unspecified application

Species

Unspecified reactive species

Melody Lok-Yi Chan,Sammy Wing-Ming Shiu,Ching-Lung Cheung,Anskar Yu-Hung Leung,Kathryn Choon-Beng Tan

Aging 12:2974-2991 PubMed32039833

2020

Long non-coding RNA TUSC8 inhibits breast cancer growth and metastasis via miR-190b-5p/MYLIP axis.

Applications

Unspecified application

Species

Unspecified reactive species

Luqing Zhao,Yangying Zhou,Yuelong Zhao,Qingling Li,Jianhua Zhou,Yitao Mao

Chemical science 9:5957-5966 PubMed30079210

2018

Inhibition of low-density lipoprotein receptor degradation with a cyclic peptide that disrupts the homodimerization of IDOL E3 ubiquitin ligase.

Applications

Unspecified application

Species

Unspecified reactive species

Eilidh K Leitch,Nagarajan Elumalai,Maria Fridén-Saxin,Göran Dahl,Paul Wan,Paul Clarkson,Eric Valeur,Garry Pairaudeau,Helen Boyd,Ali Tavassoli

Oncotarget 9:17645-17655 PubMed29707137

2018

CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Saya Ito,Akihisa Ueno,Takashi Ueda,Hideo Nakagawa,Hidefumi Taniguchi,Naruhiro Kayukawa,Atsuko Fujihara-Iwata,Fumiya Hongo,Koji Okihara,Osamu Ukimura

Oncotarget 8:64330-64343 PubMed28969074

2017

miR-19b promotes breast cancer metastasis through targeting MYLIP and its related cell adhesion molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Luqing Zhao,Yuelong Zhao,Yanong He,Yitao Mao

Molecular medicine reports 11:2329-33 PubMed25394495

2014

Docosahexanoic acid modifies low-density lipoprotein receptor abundance in HepG2 cells via suppression of the LXRα-Idol pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhou,Yue Guo,Xiaodong Zhuang,Zhimin Du

The Journal of biological chemistry 288:29613-20 PubMed23990472

2013

Reciprocal regulation of very low density lipoprotein receptors (VLDLRs) in neurons by brain-derived neurotrophic factor (BDNF) and Reelin: involvement of the E3 ligase Mylip/Idol.

Applications

WB

Species

Rat

Hai Thi Do,Céline Bruelle,Timofey Tselykh,Pilvi Jalonen,Laura Korhonen,Dan Lindholm
View all publications
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Product promise

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