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AB238595

Anti-IDUA antibody

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(1 Publication)

Rabbit Polyclonal IDUA antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IDUA.

View Alternative Names

Alpha-L-iduronidase, IDUA

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDUA antibody (AB238595)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDUA antibody (AB238595)

Paraffin-embedded human kidney tissue stained for IDUA using ab238595 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDUA antibody (AB238595)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDUA antibody (AB238595)

Paraffin-embedded human adrenal gland tissue stained for IDUA using ab238595 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDUA antibody (AB238595)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDUA antibody (AB238595)

Paraffin-embedded human prostate cancer tissue stained for IDUA using ab238595 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDUA antibody (AB238595)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDUA antibody (AB238595)

Paraffin-embedded human pancreas tissue stained for IDUA using ab238595 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IDUA.

P35475

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/500", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IDUA also known as alpha-L-iduronidase functions as an enzyme responsible for the breakdown of glycosaminoglycans. Its mechanical role involves the hydrolysis of the terminal α-L-iduronic acid residues essential in the degradation process of heparan sulfate and dermatan sulfate. The average molecular mass of IDUA is approximately 72 kDa. It expresses predominantly in lysosomes the cell's compartments that deal with digestion and waste processing. IDUA performs its functions most notably in tissues with high turnover of glycosaminoglycans such as liver spine and connective tissue.
Biological function summary

Alpha-L-iduronidase plays an important role in maintaining normal cellular operations by catalyzing important steps of the lysosomal degradation pathway. It is not part of a complex but functions independently within the lysosome. Through its enzymatic activity IDUA prevents the accumulation of undegraded molecules which could otherwise disrupt cellular function. The proper function of IDUA maintains cellular homeostasis illustrating its necessity for regular cellular maintenance.

Pathways

IDUA integrates most prominently into the lysosomal storage pathway due to its fundamental role in glycosaminoglycan degradation. This pathway includes other related enzymes like iduronate-2-sulfatase which work synergistically to ensure the complete breakdown of glycosaminoglycans. Another notable pathway where IDUA plays a part is the catabolism of carbohydrates linking it to various carbohydrate-active enzymes that collaboratively process complex sugar molecules.

Mutations or deficiencies in IDUA can result in significant health conditions such as Mucopolysaccharidosis type I (MPS I) which includes Hurler Hurler-Scheie and Scheie syndromes. These disorders arise from the accumulation of heparan sulfate and dermatan sulfate leading to a wide range of symptoms affecting multiple organs. IDUA's dysfunction connects to another enzyme iduronate-2-sulfatase which when also deficient could further exacerbate the accumulation of glycosaminoglycans and worsen clinical outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information IDUA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 7:eabf6063 PubMed34705506

2021

An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Molly M Wilson,Céline Callens,Matthieu Le Gallo,Svetlana Mironov,Qiong Ding,Amandine Salamagnon,Tony E Chavarria,Roselyne Viel,Abena D Peasah,Arjun Bhutkar,Sophie Martin,Florence Godey,Patrick Tas,Hong Soon Kang,Philippe P Juin,Anton M Jetten,Jane E Visvader,Robert A Weinberg,Massimo Attanasio,Claude Prigent,Jacqueline A Lees,Vincent J Guen
View all publications
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