Anti-IDUA antibody antibody [EPR26926-251]

13 product images

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  Immunocytochemistry/ Immunofluorescence - Anti-IDUA antibody antibody [EPR26926-251] (ab323701)

  IDUA Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-IDUA antibody

  Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized IMR-90 (human lung fibroblast) cells labelling IDUA with ab323701 at 1/50 (10.2 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

  Confocal image showing lysosome staining in IMR-90 cells(shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Low expression: JAR.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Anti-LAMP1 antibody [H4A3] - Lysosome Marker ab25630 Anti-LAMP1 mouse monoclonal antibody was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

  Counter stain secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) ab150115 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 2ug/ml dilution.

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  Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701)

  IDUA Western blot staining using rabbit Anti-IDUA antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701) at 1/1000 dilution

  Lane 1: McA-RH7777 (rat liver epithelial cell) whole cell lysate at 20 µg

  Lane 2: NMuLi (mouse liver epithelial cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Exposure time: 180s

• 

  Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701)

  IDUA Western blot staining using rabbit Anti-IDUA antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Low expression: JEG-3, JAR

  The identity of the bands between 15 kDa and 37 kDa are unknown.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  Exposure time: Lane 1: 3 seconds, Lanes 2-5: 10 seconds, Lane 6: 70 seconds

  All lanes: Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701) at 1/1000 dilution

  Lane 1: IMR-90 (human lung fibroblast) whole cell lysate at 50 µg

  Lane 2: U266B1 (human multiple myeloma B lymphocyte) whole cell lysate at 50 µg

  Lane 3: JEG-3 (human placenta epithelial cell) whole cell lysate at 50 µg

  Lane 4: JAR (human placenta choriocarcinoma epithelial cell) whole cell lysate at 50 µg

  Lane 5: HepG2 (human hepatocelullar carcinoma epithelial cell) whole cell lysate at 50 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 60-90 kDa, 36 kDa

• 

  Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701)

  IDUA Western blot staining using rabbit Anti-IDUA antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  The identity of the bands between 15 kDa and 50 kDa are unknown.

  All lanes: Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701) at 1/1000 dilution

  Lane 1: Human pancreas tissue lysate at 50 µg

  Lane 2: Human breast tissue lysate at 50 µg

  Lane 3: Human colon tissue lysate at 50 µg

  Lane 4: Human hippocampus tissue lysate at 50 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 60-90 kDa

  Exposure time: 48s

• 

  Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701)

  IDUA Western blot staining using rabbit Anti-IDUA antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701) at 1/1000 dilution

  Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 50 µg

  Lane 2: HeLa transfected with siRNA specifically targeting IDUA whole cell lysate at 50 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 60-90 kDa, 36 kDa

  Exposure time: 37s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDUA antibody antibody [EPR26926-251] (ab323701)

  IDUA Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human skeletal muscle using rabbit Anti-IDUA antibody

  Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling IDUA with ab323701 at 1/100 (5.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Low expression: weak staining on human skeletal muscle. The section was incubated with ab323701 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDUA antibody antibody [EPR26926-251] (ab323701)

  IDUA Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human pancreas using rabbit Anti-IDUA antibody

  Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling IDUA with ab323701 at 1/100 (5.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on human pancreas. The section was incubated with ab323701 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDUA antibody antibody [EPR26926-251] (ab323701)

  IDUA Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human kidney using rabbit Anti-IDUA antibody

  Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling IDUA with ab323701 at 1/100 (5.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on human kidney. The section was incubated with ab323701 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701)

  IDUA Western blot staining using rabbit Anti-IDUA antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Low expression: F9, MEF

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701) at 1/1000 dilution

  Lane 1: mIMCD3 (mouse inner medullary collecting duct epithelial cell) whole cell lysate at 50 µg

  Lane 2: F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 50 µg

  Lane 3: MEF (mouse embryo fibroblast) whole cell lysate at 50 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Exposure time: 59s

• 

  Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701)

  IDUA Western blot staining using rabbit Anti-IDUA antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  To minimize protein degradation, cells/tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

  All lanes: Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701) at 1/1000 dilution

  Lane 1: Rat testis tissue lysate at 20 µg

  Lane 2: Rat spleen tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Exposure time: 136s

• 

  Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701)

  IDUA Western blot staining using rabbit Anti-IDUA antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  The identity of the bands between 15 kDa and 50 kDa are unknown.

  Exposure time: Lanes 1, 3-4: 81 seconds, Lane 2: 103 seconds

  All lanes: Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701) at 1/1000 dilution

  Lane 1: Mouse pancreas tissue lysate at 50 µg

  Lane 2: Rat pancreas tissue lysate at 50 µg

  Lane 3: Rat colon tissue lysate at 50 µg

  Lane 4: Rat brain tissue lysate at 50 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

• 

  Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701)

  IDUA Western blot staining using rabbit Anti-IDUA antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701) at 1/1000 dilution

  Lane 1: mIMCD3 (mouse inner medullary collecting duct epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

  Lane 2: mIMCD3 transfected with siRNA specifically targeting IDUA whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Exposure time: 8s

• 

  Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701)

  IDUA Western blot staining using rabbit Anti-IDUA antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Low expression: skeletal muscle

  To minimize protein degradation, cells/tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-IDUA antibody antibody [EPR26926-251] (ab323701) at 1/1000 dilution

  Lane 1: Rat lung tissue lysate at 20 µg

  Lane 2: Rat skeletal muscle tissue lysate at 20 µg

  Lane 3: Rat kidney tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Exposure time: 70s