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AB309234

Anti-IDUA antibody [EPR26926-80] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal IDUA antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Alpha-L-iduronidase, IDUA

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Sandwich ELISA - Anti-IDUA antibody [EPR26926-80] - BSA and Azide free (Capture) (AB309234)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-IDUA antibody [EPR26926-80] - BSA and Azide free (Capture) (AB309234)

Sandwich ELISA of ab309269 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26926-80

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IDUA also known as alpha-L-iduronidase functions as an enzyme responsible for the breakdown of glycosaminoglycans. Its mechanical role involves the hydrolysis of the terminal α-L-iduronic acid residues essential in the degradation process of heparan sulfate and dermatan sulfate. The average molecular mass of IDUA is approximately 72 kDa. It expresses predominantly in lysosomes the cell's compartments that deal with digestion and waste processing. IDUA performs its functions most notably in tissues with high turnover of glycosaminoglycans such as liver spine and connective tissue.
Biological function summary

Alpha-L-iduronidase plays an important role in maintaining normal cellular operations by catalyzing important steps of the lysosomal degradation pathway. It is not part of a complex but functions independently within the lysosome. Through its enzymatic activity IDUA prevents the accumulation of undegraded molecules which could otherwise disrupt cellular function. The proper function of IDUA maintains cellular homeostasis illustrating its necessity for regular cellular maintenance.

Pathways

IDUA integrates most prominently into the lysosomal storage pathway due to its fundamental role in glycosaminoglycan degradation. This pathway includes other related enzymes like iduronate-2-sulfatase which work synergistically to ensure the complete breakdown of glycosaminoglycans. Another notable pathway where IDUA plays a part is the catabolism of carbohydrates linking it to various carbohydrate-active enzymes that collaboratively process complex sugar molecules.

Mutations or deficiencies in IDUA can result in significant health conditions such as Mucopolysaccharidosis type I (MPS I) which includes Hurler Hurler-Scheie and Scheie syndromes. These disorders arise from the accumulation of heparan sulfate and dermatan sulfate leading to a wide range of symptoms affecting multiple organs. IDUA's dysfunction connects to another enzyme iduronate-2-sulfatase which when also deficient could further exacerbate the accumulation of glycosaminoglycans and worsen clinical outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information IDUA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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