Rabbit Recombinant Monoclonal IER3IP1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Images
![Western blot - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (AB250460), expandable thumbnail](https://content.abcam.com/products/images/ier3ip1-antibody-epr13440-bsa-and-azide-free-ab250460--western-blot-img54869.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (AB250460)")
![Immunoprecipitation - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (AB250460), expandable thumbnail](https://content.abcam.com/products/images/ier3ip1-antibody-epr13440-bsa-and-azide-free-ab250460--immunoprecipitation-img39512.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (AB250460)")
![Immunocytochemistry/ Immunofluorescence - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (AB250460), expandable thumbnail](https://content.abcam.com/products/images/ier3ip1-antibody-epr13440-bsa-and-azide-free-ab250460--immunocytochemistry-immunofluorescence-img7385.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (AB250460)")
![Flow Cytometry (Intracellular) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (AB250460), expandable thumbnail](https://content.abcam.com/products/images/ier3ip1-antibody-epr13440-bsa-and-azide-free-ab250460--flow-cytometry-intracellular-img85323.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (AB250460)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (AB250460), expandable thumbnail](https://content.abcam.com/products/images/ier3ip1-antibody-epr13440-bsa-and-azide-free-ab250460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125702.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (AB250460)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Regulator of endoplasmic reticulum secretion that acts as a key determinant of brain size (PubMed:33122427). Required for secretion of extracellular matrix proteins (PubMed:33122427). Required for correct brain development by depositing sufficient extracellular matrix proteins for tissue integrity and the proliferation of neural progenitors (PubMed:33122427). Acts as a regulator of the unfolded protein response (UPR) (By similarity).
Alternative names
HSPC039, IER3IP1, Immediate early response 3-interacting protein 1
Recommended products
Rabbit Recombinant Monoclonal IER3IP1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13440
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250460 is the carrier-free version of Anti-IER3IP1 antibody [EPR13440] ab181247.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IER3IP1 also known as Immediate Early Response 3 Interacting Protein 1 is a small protein with a mass of approximately 11 kDa. It primarily localizes within the endoplasmic reticulum (ER) and shows expression in various tissues including the brain liver and pancreas. IER3IP1 plays a mechanical role in protein trafficking and the regulation of ER stress affecting cellular responses to environmental changes.
- Biological function summary
IER3IP1 participates in processes related to cellular stress response and protein folding. It acts as a component involved in maintaining ER function possibly engaging in a protein complex to modulate its activity. This modulation is essential for controlling apoptosis and cell survival suggesting its involvement in critical cellular processes. The protein's interactions help sustain proper protein synthesis and folding within the ER.
- Pathways
IER3IP1 interacts within the unfolded protein response pathway maintaining ER homeostasis. It engages with proteins like GRP78 and ATF6 which are significant in managing ER stress and protein folding pathways. These interactions demonstrate IER3IP1's importance in cellular mechanisms that protect cells from stress-induced damages.
- Associated diseases and disorders
Mutations or misregulation of IER3IP1 correlate with microcephaly and epilepsy. Research indicates its connection with proteins involved in neurodevelopmental disorders possibly interacting with molecules like Reelin or Dystrophin. This relationship highlights the significance of IER3IP1 in maintaining neural cell health and suggests its potential as a target for therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460), expandable thumbnail](https://content.abcam.com/products/images/ier3ip1-antibody-epr13440-bsa-and-azide-free-ab250460--western-blot-img54869.jpg "Western blot - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)")
Western blot - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
This data was developed using Anti-IER3IP1 antibody [EPR13440] ab181247, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-IER3IP1 antibody [EPR13440] (Anti-IER3IP1 antibody [EPR13440] ab181247) at 1/1000 dilution
Lane 1: HepG2 lysate at 20 µg
Lane 2: HeLa lysate at 20 µg
Lane 3: Human fetal heart lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 9 kDa
![Immunoprecipitation - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460), expandable thumbnail](https://content.abcam.com/products/images/ier3ip1-antibody-epr13440-bsa-and-azide-free-ab250460--immunoprecipitation-img39512.jpg "Immunoprecipitation - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)")
Immunoprecipitation - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
This data was developed using Anti-IER3IP1 antibody [EPR13440] ab181247, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using Anti-IER3IP1 antibody [EPR13440] ab181247 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000.All lanes: Immunoprecipitation - Anti-IER3IP1 antibody [EPR13440] (Anti-IER3IP1 antibody [EPR13440] ab181247)
Predicted band size: 9 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460), expandable thumbnail](https://content.abcam.com/products/images/ier3ip1-antibody-epr13440-bsa-and-azide-free-ab250460--immunocytochemistry-immunofluorescence-img7385.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)")
Immunocytochemistry/ Immunofluorescence - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
This data was developed using Anti-IER3IP1 antibody [EPR13440] ab181247, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling IER3IP1 with Anti-IER3IP1 antibody [EPR13440] ab181247 at 1/250 dilution, dollowed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution and DAPI staining (blue).
![Flow Cytometry (Intracellular) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460), expandable thumbnail](https://content.abcam.com/products/images/ier3ip1-antibody-epr13440-bsa-and-azide-free-ab250460--flow-cytometry-intracellular-img85323.jpg "Flow Cytometry (Intracellular) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)")
Flow Cytometry (Intracellular) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
This data was developed using Anti-IER3IP1 antibody [EPR13440] ab181247, the same antibody clone in a different buffer formulation.
Intracellular flow cytometricl analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling IER3IP1 with Anti-IER3IP1 antibody [EPR13440] ab181247 at 1/210 (red) compared to a negative control antibody (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460), expandable thumbnail](https://content.abcam.com/products/images/ier3ip1-antibody-epr13440-bsa-and-azide-free-ab250460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125702.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
This data was developed using Anti-IER3IP1 antibody [EPR13440] ab181247, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling IER3IP1 with Anti-IER3IP1 antibody [EPR13440] ab181247 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460), expandable thumbnail](https://content.abcam.com/products/images/ier3ip1-antibody-epr13440-bsa-and-azide-free-ab250460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113462.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
This data was developed using Anti-IER3IP1 antibody [EPR13440] ab181247, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling IER3IP1 with Anti-IER3IP1 antibody [EPR13440] ab181247 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460), expandable thumbnail](https://content.abcam.com/products/images/ier3ip1-antibody-epr13440-bsa-and-azide-free-ab250460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113462.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)")
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