AB65152

Anti-IEX1/IER3 antibody

Name: Anti-IEX1/IER3 antibody (ab65152) | Abcam
Brand: Abcam Limited
SKU: AB65152
Price: 620 USD
Availability: InStock

Be the first to review this product! Submit a review

(7 Publications)

Rabbit Polyclonal IEX1/IER3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 7 publications.

View Alternative Names

DIF2, IEX1, PRG1, IER3, Radiation-inducible immediate-early gene IEX-1, Differentiation-dependent gene 2 protein, Immediate early protein GLY96, Immediate early response 3 protein, PACAP-responsive gene 1 protein, Protein DIF-2, Protein PRG1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-IEX1/IER3 antibody (AB65152)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IEX1/IER3 antibody (AB65152)

Immunocytochemistry/ Immunofluorescence analysis of human brain cells staining IEX1/IER3 with ab65152 at 20 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IEX1/IER3 antibody (AB65152)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IEX1/IER3 antibody (AB65152)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human adrenal tissue, staining IEX1/IER3 with ab65152 at 5 μg/ml.

Supplier Data

Western blot - Anti-IEX1/IER3 antibody (AB65152)

Lane 1:

Western blot - Anti-IEX1/IER3 antibody (ab65152) at 2 µg/mL

Lane 2:

Western blot - Anti-IEX1/IER3 antibody (ab65152) at 4 µg/mL

All lanes:

Human brain tissue lysate

Predicted band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Immediate Early Response 3 (IER3) also known as IEX1 is an early-response gene product with a mass of approximately 19 kDa. It expresses in various tissues including the skin liver and immune system cells notably in T lymphocytes. IER3 regulates cellular processes such as growth and differentiation by influencing cellular apoptosis and survival mechanisms.

Biological function summary

Immediate Early Response 3 plays a significant role in cellular stress response and immune signaling. IER3 does not form a traditional complex but interacts with intracellular signaling molecules to modulate apoptosis. It also influences the activity of MAPK and NF-kB pathways critical for stress response and inflammation. Through this modulation IER3 directly impacts cell fate under stress conditions.

Pathways

Immediate Early Response 3 is involved in modulating cellular survival and stress response pathways. It interacts with the MAPK signaling pathway which regulates cell growth division and stress response. IER3 influences pathways involving NF-kB a protein complex controlling transcription of DNA and cytokine production. These pathways connect IER3 to other proteins such as Bcl-2 and caspases modifying cellular responses.

Immediate Early Response 3 associates with cancer progression and autoimmune diseases. Its expression levels can influence the development of certain cancers by altering apoptosis and cell proliferation rates. In autoimmune disorders IER3 affects immune responses being linked to proteins like NF-kB which regulates inflammation. Understanding the role of IER3 could aid in developing therapies for conditions like rheumatoid arthritis or certain cancer types.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment.

See full target information IER3

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Chinese medical journal 136:1177-1187 PubMed37083129

2023

Single-cell RNA sequencing reveals the transcriptomic landscape of kidneys in patients with ischemic acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Tang,Peng Jin,Chanjuan Shen,Wei Lin,Leilin Yu,Xueling Hu,Ting Meng,Linlin Zhang,Ling Peng,Xiangcheng Xiao,Peter Eggenhuizen,Joshua D Ooi,Xueqin Wu,Xiang Ding,Yong Zhong

International journal of molecular sciences 18: PubMed28353642

2017

Dysregulated IER3 Expression is Associated with Enhanced Apoptosis in Titin-Based Dilated Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Qifeng Zhou,Julia Kelley Hahn,Balram Neupane,Parwez Aidery,Siegfried Labeit,Meinrad Gawaz,Michael Gramlich

Scientific reports 6:24135 PubMed27063893

2016

IEX-1 deficiency induces browning of white adipose tissue and resists diet-induced obesity.

Applications

IHC

Species

Unspecified reactive species

Mohd Shahid,Ammar A Javed,David Chandra,Haley E Ramsey,Dilip Shah,Mohammed F Khan,Liping Zhao,Mei X Wu

International journal of molecular medicine 31:959-66 PubMed23403649

2013

Identification of immediate early gene X-1 as a cellular target gene of hcmv-mir-UL148D.

Applications

Unspecified application

Species

Unspecified reactive species

Yue-Ping Wang,Ying Qi,Yu-Jing Huang,Man-Long Qi,Yan-Ping Ma,Rong He,Yao-Hua Ji,Zheng-Rong Sun,Qiang Ruan

Clinical cancer research : an official journal of 17:1935-46 PubMed21307145

2011

Lenalidomide restrains motility and overangiogenic potential of bone marrow endothelial cells in patients with active multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Annunziata De Luisi,Arianna Ferrucci,Addolorata M L Coluccia,Roberto Ria,Michele Moschetta,Emanuela de Luca,Luisa Pieroni,Michele Maffia,Andrea Urbani,Giulia Di Pietro,Attilio Guarini,Girolamo Ranieri,Paolo Ditonno,Simona Berardi,Antonella Caivano,Antonio Basile,Nicola Cascavilla,Silvana Capalbo,Giovanni Quarta,Franco Dammacco,Domenico Ribatti,Angelo Vacca

Clinical cancer research : an official journal of 15:7161-9 PubMed19920113

2009

Gene expression profiles classify human osteosarcoma xenografts according to sensitivity to doxorubicin, cisplatin, and ifosfamide.

Applications

Species

Mouse

Skjalg Bruheim,Yaguang Xi,Jingfang Ju,Oystein Fodstad

Clinical cancer research : an official journal of 15:5369-78 PubMed19690192

2009

Gene expression profiling of bone marrow endothelial cells in patients with multiple myeloma.

Applications

Species

Human

Roberto Ria,Katia Todoerti,Simona Berardi,Addolorata Maria Luce Coluccia,Annunziata De Luisi,Michela Mattioli,Domenica Ronchetti,Fortunato Morabito,Attilio Guarini,Maria Teresa Petrucci,Franco Dammacco,Domenico Ribatti,Antonino Neri,Angelo Vacca

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IEX1/IER3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-IEX1/IER3 antibody (AB65152)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IEX1/IER3 antibody (AB65152)

Western blot - Anti-IEX1/IER3 antibody (AB65152)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise