AB279341

Anti-IFFO1 antibody [EPR22711-61] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal IFFO1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

IFFO, IFFO1, Non-homologous end joining factor IFFO1, NHEJ factor IFFO1, Intermediate filament family orphan 1, Tumor antigen HOM-TES-103

3 Images

Collaborator

Western blot - Anti-IFFO1 antibody [EPR22711-61] - BSA and Azide free (AB279341)

This data was developed using ab242130, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Data was kindly provided by an anonymous collaborator.

The antibody for IFFO2 is ab242131.

Lanes 1 - 4:

Western blot - Anti-IFFO2 antibody [EPR22712-35] (<a href='/en-us/products/primary-antibodies/iffo2-antibody-epr22712-35-ab242131'>ab242131</a>) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-IFFO1 antibody [EPR22711-61] (<a href='/en-us/products/primary-antibodies/iffo1-antibody-epr22711-61-ab242130'>ab242130</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (human embryonic kidney epithelial cell) whole cell lysate

Lane 2:

IFFO1 knockout HEK-293T whole cell lysate

Lane 3:

IFFO2 knockout HEK-293T whole cell lysate

Lane 4:

IFFO1/IFFO2 knockout HEK-293T whole cell lysate

Predicted band size: 57 kDa,62 kDa

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Supplier Data

Immunoprecipitation - Anti-IFFO1 antibody [EPR22711-61] - BSA and Azide free (AB279341)

This data was developed using ab242130, the same antibody clone in a different buffer formulation.

IFFO1 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate with ab242130 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab242130 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug

Lane 2 : ab242130 IP in HEK-293T whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab242130 in HEK-293T whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 41 seconds

All lanes:

Immunoprecipitation - Anti-IFFO1 antibody [EPR22711-61] (<a href='/en-us/products/primary-antibodies/iffo1-antibody-epr22711-61-ab242130'>ab242130</a>)

Observed band size: 75 kDa

false

Lab

Western blot - Anti-IFFO1 antibody [EPR22711-61] - BSA and Azide free (AB279341)

This data was developed using ab242130, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The observed MW is consistent with what has been described in the literature (PMID : 31548606).

Negative control : HeLa (PMID : 31548606).

Exposure time : 3 minutes

All lanes:

Western blot - Anti-IFFO1 antibody [EPR22711-61] (<a href='/en-us/products/primary-antibodies/iffo1-antibody-epr22711-61-ab242130'>ab242130</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HDLM-2 (human hodgkin lymphoma) whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 62 kDa

Observed band size: 75 kDa

false

Unconjugated

Anti-IFFO1 antibody [EPR22711-61]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22711-61

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab279341 is the carrier-free version of ab242130.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IFFO1 also known as Intermediate Filament Family Orphan 1 is a protein that plays a role in structural support in cells. It weighs approximately 63 kDa. IFFO1 is expressed in various tissues with a higher concentration in the heart and skeletal muscles and also found in the liver and testis. This target is involved in maintaining the integrity of the cytoskeleton by interacting with other cytoskeletal proteins.

Biological function summary

The protein supports cellular architecture and dynamics during cell division and differentiation. IFFO1 can interact with other intermediate filament proteins but researchers suggest it may also function independently. Though not very well-studied it contributes to the stability of cells' mechanical properties playing a part in maintaining cellular organization and providing resilience against mechanical stress.

Pathways

Its involvement in cytoskeletal organization connects it with the cytoskeletal remodeling pathway which is fundamental for cellular shape and stability. It associates with proteins like vimentin and desmin which are important in this pathway. These interactions underline its contribution to cell structure and mechanics connecting it to both cellular movement and morphological changes.

Evidence links IFFO1 to muscular dystrophy where muscle integrity and function diminish and perhaps to certain liver conditions. The protein interacts with key structural entities like desmin which is also implicated in these disorders. Dysregulation of IFFO1 or its interacting partners may contribute to the disruption of muscle and liver function leading to progressive muscle disorders or hepatic pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nuclear matrix protein involved in the immobilization of broken DNA ends and the suppression of chromosome translocation during DNA double-strand breaks (DSBs) (PubMed : 31548606). Interacts with the nuclear lamina component LMNA, resulting in the formation of a nucleoskeleton that relocalizes to the DSB sites in a XRCC4-dependent manner and promotes the immobilization of the broken ends, thereby preventing chromosome translocation (PubMed : 31548606). Acts as a scaffold that allows the DNA repair protein XRCC4 and LMNA to assemble into a complex at the DSB sites (PubMed : 31548606).

See full target information IFFO1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IFFO1 antibody [EPR22711-61] - BSA and Azide free

Western blot - Anti-IFFO1 antibody [EPR22711-61] - BSA and Azide free (AB279341)

Immunoprecipitation - Anti-IFFO1 antibody [EPR22711-61] - BSA and Azide free (AB279341)

Western blot - Anti-IFFO1 antibody [EPR22711-61] - BSA and Azide free (AB279341)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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