AB242131

Anti-IFFO2 antibody [EPR22712-35]

20ul selling size
RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal IFFO2 antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples.

View Alternative Names

Intermediate filament family orphan 2, IFFO2

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Supplier Data

Immunoprecipitation - Anti-IFFO2 antibody [EPR22712-35] (AB242131)

IFFO2 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate with ab242131 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab242131 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug

Lane 2 : ab242131 IP in HEK293T whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab242131 in HEK-293T whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-IFFO2 antibody [EPR22712-35] (ab242131)

Predicted band size: 57 kDa

Observed band size: 70 kDa

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Lab

Western blot - Anti-IFFO2 antibody [EPR22712-35] (AB242131)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lane 2 : 15 seconds Lane 1, lane 3-5 : 59 seconds

All lanes:

Western blot - Anti-IFFO2 antibody [EPR22712-35] (ab242131) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HDLM-2 (human hodgkin lymphoma) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate at 20 µg

Lane 5:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 57 kDa

Observed band size: 70 kDa

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Collaborator

Western blot - Anti-IFFO2 antibody [EPR22712-35] (AB242131)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Data was kindly provided by an anonymous collaborator.

The antibody for IFFO1 is ab242130.

Lanes 1 - 4:

Western blot - Anti-IFFO2 antibody [EPR22712-35] (ab242131) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-IFFO1 antibody [EPR22711-61] (<a href='/en-us/products/primary-antibodies/iffo1-antibody-epr22711-61-ab242130'>ab242130</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (human embryonic kidney epithelial cell) whole cell lysate

Lane 2:

IFFO1 knockout HEK-293T whole cell lysate

Lane 3:

IFFO2 knockout HEK-293T whole cell lysate

Lane 4:

IFFO1/IFFO2 knockout HEK-293T whole cell lysate

Predicted band size: 57 kDa,62 kDa

false

Carrier free

Anti-IFFO2 antibody [EPR22712-35] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22712-35

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IFFO2 also known as Interferon-Induced Protein with Tetratricopeptide Repeats 2 is a protein that typically weighs approximately 110 kDa. It localizes mainly in the cytoplasm and nucleus expressing in various tissues with higher presence in immune tissues and the brain. This protein participates in cellular signaling processes by interacting with other molecular complexes and proteins through its tetratricopeptide repeat domains. The role of IFFO2 in these regions suggests a part in coordinating cellular responses to external stimuli.

Biological function summary

IFFO2 contributes to immune modulation and cellular stress response. It associates with larger protein complexes aiding in the regulation of gene expression and protein stability. IFFO2 influences how cells react to viral infections through its interactions adapting cellular processes to enhance responsiveness to interferons. This dimension of functionality underlines its involvement in defensive mechanisms against pathogens showcasing its significance in immune regulation networks.

Pathways

The protein interacts within the interferon signaling pathway and plays a part in the regulation of apoptosis. IFFO2 relates to interferon regulatory factor 3 (IRF3) aiding in the enhancement of antiviral defenses. Additionally it may affect the expression of other important signaling molecules such as NF-kB showing its coordination role in controlling cell fate decisions. Its involvement in these pathways places it among the core regulators of immune and stress-response dynamics.

IFFO2 relates to neurodegenerative disorders and cancer. Its interaction with p53 protein points to its role in tumor suppression as it influences cell cycle arrest and apoptosis. Alterations in IFFO2 might contribute to pathological developments like Alzheimer's disease where disrupted immune function and protein aggregation are notable. Understanding IFFO2's regulatory impact could provide new insights into therapeutic strategies against these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information IFFO2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IFFO2 antibody [EPR22712-35]

Immunoprecipitation - Anti-IFFO2 antibody [EPR22712-35] (AB242131)

Western blot - Anti-IFFO2 antibody [EPR22712-35] (AB242131)

Western blot - Anti-IFFO2 antibody [EPR22712-35] (AB242131)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise