Rabbit Recombinant Monoclonal IFFO2 antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples.
Images
![Immunoprecipitation - Anti-IFFO2 antibody [EPR22712-35] (AB242131), expandable thumbnail](https://content.abcam.com/products/images/iffo2-antibody-epr22712-35-ab242131--immunoprecipitation-img6106.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IFFO2 antibody [EPR22712-35] (AB242131)")
![Western blot - Anti-IFFO2 antibody [EPR22712-35] (AB242131), expandable thumbnail](https://content.abcam.com/products/images/iffo2-antibody-epr22712-35-ab242131--western-blot-img13433.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IFFO2 antibody [EPR22712-35] (AB242131)")
![Western blot - Anti-IFFO2 antibody [EPR22712-35] (AB242131), expandable thumbnail](https://content.abcam.com/products/images/iffo2-antibody-epr22712-35-ab242131--western-blot-img165.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IFFO2 antibody [EPR22712-35] (AB242131)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Expected | Tested | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Alternative names
Intermediate filament family orphan 2, IFFO2
Recommended products
Rabbit Recombinant Monoclonal IFFO2 antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22712-35
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IFFO2 also known as Interferon-Induced Protein with Tetratricopeptide Repeats 2 is a protein that typically weighs approximately 110 kDa. It localizes mainly in the cytoplasm and nucleus expressing in various tissues with higher presence in immune tissues and the brain. This protein participates in cellular signaling processes by interacting with other molecular complexes and proteins through its tetratricopeptide repeat domains. The role of IFFO2 in these regions suggests a part in coordinating cellular responses to external stimuli.
- Biological function summary
IFFO2 contributes to immune modulation and cellular stress response. It associates with larger protein complexes aiding in the regulation of gene expression and protein stability. IFFO2 influences how cells react to viral infections through its interactions adapting cellular processes to enhance responsiveness to interferons. This dimension of functionality underlines its involvement in defensive mechanisms against pathogens showcasing its significance in immune regulation networks.
- Pathways
The protein interacts within the interferon signaling pathway and plays a part in the regulation of apoptosis. IFFO2 relates to interferon regulatory factor 3 (IRF3) aiding in the enhancement of antiviral defenses. Additionally it may affect the expression of other important signaling molecules such as NF-kB showing its coordination role in controlling cell fate decisions. Its involvement in these pathways places it among the core regulators of immune and stress-response dynamics.
- Associated diseases and disorders
IFFO2 relates to neurodegenerative disorders and cancer. Its interaction with p53 protein points to its role in tumor suppression as it influences cell cycle arrest and apoptosis. Alterations in IFFO2 might contribute to pathological developments like Alzheimer's disease where disrupted immune function and protein aggregation are notable. Understanding IFFO2's regulatory impact could provide new insights into therapeutic strategies against these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-IFFO2 antibody [EPR22712-35] (ab242131), expandable thumbnail](https://content.abcam.com/products/images/iffo2-antibody-epr22712-35-ab242131--immunoprecipitation-img6106.jpg "Immunoprecipitation - Anti-IFFO2 antibody [EPR22712-35] (ab242131)")
Immunoprecipitation - Anti-IFFO2 antibody [EPR22712-35] (ab242131)
IFFO2 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate with ab242131 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab242131 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug
Lane 2: ab242131 IP in HEK293T whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab242131 in HEK-293T whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
All lanes: Immunoprecipitation - Anti-IFFO2 antibody [EPR22712-35] (ab242131)
Predicted band size: 57 kDa
Observed band size: 70 kDa
![Western blot - Anti-IFFO2 antibody [EPR22712-35] (ab242131), expandable thumbnail](https://content.abcam.com/products/images/iffo2-antibody-epr22712-35-ab242131--western-blot-img13433.jpg "Western blot - Anti-IFFO2 antibody [EPR22712-35] (ab242131)")
Western blot - Anti-IFFO2 antibody [EPR22712-35] (ab242131)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Data was kindly provided by an anonymous collaborator.
The antibody for IFFO1 is Anti-IFFO1 antibody [EPR22711-61] ab242130.
Lanes 1 - 4: Western blot - Anti-IFFO2 antibody [EPR22712-35] (ab242131) at 1/1000 dilution
Lanes 1 - 4: Western blot - Anti-IFFO1 antibody [EPR22711-61] (Anti-IFFO1 antibody [EPR22711-61] ab242130) at 1/1000 dilution
Lane 1: Wild-type HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2: IFFO1 knockout HEK-293T whole cell lysate
Lane 3: IFFO2 knockout HEK-293T whole cell lysate
Lane 4: IFFO1/IFFO2 knockout HEK-293T whole cell lysate
Predicted band size: 57 kDa, 62 kDa
![Western blot - Anti-IFFO2 antibody [EPR22712-35] (ab242131), expandable thumbnail](https://content.abcam.com/products/images/iffo2-antibody-epr22712-35-ab242131--western-blot-img165.jpg "Western blot - Anti-IFFO2 antibody [EPR22712-35] (ab242131)")
Western blot - Anti-IFFO2 antibody [EPR22712-35] (ab242131)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lane 2: 15 seconds Lane 1, lane 3-5: 59 seconds
All lanes: Western blot - Anti-IFFO2 antibody [EPR22712-35] (ab242131) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: HDLM-2 (human hodgkin lymphoma) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate at 20 µg
Lane 5: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 70 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com