AB249529

Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal IFI16 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

IFNGIP1, IFI16, Gamma-interferon-inducible protein 16, Ifi-16, Interferon-inducible myeloid differentiation transcriptional activator

10 Images

Flow Cytometry (Intracellular) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

This data was developed using ab169788, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of Raji (human Burkitt's lymphoma) cells labelling IFI16 (red) with ab169788 at dilution of 1/500. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde. Isotype control antibody was (ab172730) Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

This data was developed using ab169788, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) cells labelling IFI16 with ab169788 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

The image shows nuclear staining on Jurkat cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

This data was developed using ab169788, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling IFI16, using ab169788 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

This data was developed using ab169788, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of Raji cells labeling IFI16, using ab169788 at a 1/100 dilution.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

This data was developed using ab169788, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labelling IFI16 (red) with ab169788 at dilution of 1/500. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde. Isotype control antibody was (ab172730) Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

This data was developed using ab169788, the same antibody clone in a different buffer formulation.

ab169788 showing -ve staining in Human gastric adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

This data was developed using ab169788, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bone marrow tissue labeling IFI16, using ab169788 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

This data was developed using ab169788, the same antibody clone in a different buffer formulation.

ab169788 showing -ve staining in Human normal uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

This data was developed using ab169788, the same antibody clone in a different buffer formulation.

ab169788 showing +ve staining in Human normal spleen tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

This data was developed using ab169788, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-IFI16 antibody [EPR11767(B)] (<a href='/en-us/products/primary-antibodies/ifi16-antibody-epr11767b-ab169788'>ab169788</a>) at 1/1000 dilution

Lane 1:

U937 treated with IFN gamma cell lysate at 10 µg

Lane 2:

U937 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

Raji cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 88 kDa

true

Unconjugated

Anti-IFI16 antibody [EPR11767(B)]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-IFI16 antibody [EPR11767(B)]
578 PE

PE Anti-IFI16 antibody [EPR11767(B)]
660 APC

APC Anti-IFI16 antibody [EPR11767(B)]
HRP

HRP Anti-IFI16 antibody [EPR11767(B)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-IFI16 antibody [EPR11767(B)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-IFI16 antibody [EPR11767(B)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-IFI16 antibody [EPR11767(B)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-IFI16 antibody [EPR11767(B)]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-IFI16 antibody [EPR11767(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11767(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Use of a HRP/AP polymerized secondary antibody is recommended.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab249529 is the carrier-free version of ab169788.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IFI16 protein also known as Interferon γ Inducible Protein 16 is a DNA sensor belonging to the PYHIN family with a molecular mass of approximately 85 kDa. Widely expressed in the nucleus and cytoplasm its primary role involves detecting foreign DNA within cells. It possesses a pyrin domain two HIN-200 domains and also plays a role in regulating gene expression particularly in response to pathogen infection. This versatile protein can act in multiple cellular environments and its location within the cell can dictate its function.

Biological function summary

IFI16 protein participates in the innate immune response by recognizing viral and bacterial double-stranded DNA. It forms part of a signaling complex that activates pathogen recognition receptors and induces the production of type I interferons. IFI16 can interact with various DNA sensors and is known for its ability to bind DNA directly providing a defense mechanism against infection. The protein also plays a role in the regulation of transcription which contributes to cell growth and differentiation.

Pathways

IFI16 protein has significant roles in the cGAS-STING and BRCA1 pathways. It interacts with cGAS to regulate the production of cyclic GMP-AMP activating STING and promoting antiviral responses. Additionally IFI16 aligns with BRCA1 dysfunction impacting DNA repair processes. This interaction underlines its involvement in maintaining genomic integrity and modulating the immune responses along with cGAS STING and BRCA1 proteins which are critical in these pathways.

IFI16 protein plays a role in autoimmune diseases and various cancers. In Systemic Lupus Erythematosus (SLE) it can induce inflammation by recognizing self-DNA leading to chronic immune activation. In certain cancers its expression levels correlate with malignancy where it influences tumor progression and response to therapy. In these contexts IFI16 interacts closely with cGAS and BRCA1 proteins highlighting its relevance to disease mechanisms and offering potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors. Necessary to activate the IRF3 signaling cascade during human herpes simplex virus 1 (HHV-1) infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibition of viral immediate-early gene expression and replication. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.. Isoform IFI16-beta. Isoform that specifically inhibits the AIM2 inflammasome (PubMed : 30104205). Binds double-stranded DNA (dsDNA) in the cytoplasm, impeding its detection by AIM2 (PubMed : 30104205). Also prevents the interaction between AIM2 and PYCARD/ASC via its interaction with AIM2, thereby inhibiting assembly of the AIM2 inflammasome (PubMed : 30104205). This isoform also weakly induce production of type I interferon-beta (IFNB1) via its interaction with STING1 (PubMed : 30104205).

See full target information IFI16

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

Flow Cytometry (Intracellular) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

Western blot - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (AB249529)

Related conjugates and formulations (10)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise