Anti-Ifi204 antibody [EPR25256-211] (ab307201)

Rabbit Monoclonal Ifi204 antibody. Suitable for Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, WB and reacts with Mouse samples.

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Images

Western blot - Anti-Ifi204 antibody [EPR25256-211] (AB307201), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt (Intra)	ICC/IF	IHC-Fr	IHC-P	WB
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Not recommended	Tested	Tested	Tested	Tested	Tested
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat	Dilution info -	Notes -

Target data

See full target information Ifi204

Function

Interferon-stimulated protein that plays a role in several biological processes including cell differentiation, autophagy and innate immunity (PubMed:16244109, PubMed:25710914, PubMed:30936875). Cooperates with CGAS to sense dsDNA and activates the STING-dependent type I IFN pathway (PubMed:25710914, PubMed:33619523). Mechanistically, gets acetylated upon bacterial infection and then translocates from nucleus into cytoplasm to recruit STING for activation of TBK1-dependent IRF3 nuclear translocation and IFN-beta release (PubMed:28529930). Inhibits the transcription of ribosomal RNA. May inhibit DNA binding by UBTF. Inhibits cell growth via p53/TP53 and RB1-dependent and independent pathways. Acts as a coactivator of RUNX2 during osteogenesis. May be involved in macrophage differentiation. Enables skeletal muscle and cardiac myocyte differentiation by sequestring Id proteins in the cytosol and promoting their ubiquitination and subsequent degradation.

Alternative names

Interferon-activable protein 204, Ifi-204, Interferon-inducible protein p204, Ifi204

Recommended products

Rabbit Monoclonal Ifi204 antibody. Suitable for Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, WB and reacts with Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR25256-211
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Ifi204 also known as Interferon-activated gene 204 is a protein of the mouse involved in the immune response. It has a molecular mass of approximately 74 kilodaltons. This protein is expressed mainly in activated macrophages and neutrophils and contributes to the innate immune mechanism. It detects cytosolic DNA and activates the STING pathway to induce type I interferon production. It contains a HIN-200 domain that helps in recognizing specific DNA sequences.

Biological function summary

Interferon-activated gene 204 plays a role in the host defense mechanism. It functions as part of the cellular response to viral infections and potentially some bacterial infections. The protein is part of a larger complex involved in DNA sensing and it activates immune signaling pathways upon detecting foreign DNA. It interacts with components of the inflammasome participating in activating inflammatory responses necessary for pathogen clearance.

Pathways

Interferon-activated gene 204 participates in the cGAS-STING DNA sensing pathway. This pathway is essential for the production of type I interferons as a defense against viruses. The protein associates with cGAS and STING key players in the recognition of cytosolic DNA. Its role in this pathway highlights its importance in the body's first line of defense closely linking it to the activation of downstream signaling that involves IRF3 and NF-kB.

Associated diseases and disorders

Altered expression or function of Interferon-activated gene 204 has connections with autoimmunity and inflammatory diseases. Its dysregulation may lead to excessive inflammation contributing to conditions like systemic lupus erythematosus. In this context it interacts with proteins such as AIM2 which is part of the inflammasome complex involved in autoimmune inflammation. Understanding its precise role in these diseases could potentially lead to new therapeutic strategies for managing autoimmune disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Western blot - Anti-Ifi204 antibody [EPR25256-211] (ab307201)
Blocking and diluting buffer and concentration: 5% NFDM/TBST

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

Exposure time:

Lane 1: 180 seconds, Lane 2: 70 seconds.
All lanes: Western blot - Anti-Ifi204 antibody [EPR25256-211] (ab307201) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate at 20 µg
Lane 2: Mouse spleen tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 82 kDa
Exposure time: 180s
Flow Cytometry (Intracellular) - Anti-Ifi204 antibody [EPR25256-211] (ab307201)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MEF (mouse embryonic fibroblast (immortalized)) cells labeling Ifi204 with ab307201 at 1/50 dilution (1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-Ifi204 antibody [EPR25256-211] (ab307201)
Blocking and diluting buffer and concentration: 5% NFDM/TBST

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

Exposure time: 59 seconds
All lanes: Western blot - Anti-Ifi204 antibody [EPR25256-211] (ab307201) at 1/1000 dilution
Lane 1: MEF transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: MEF transfected with siRNA specifically targeting Ifi204 whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 82 kDa
Exposure time: 59s
Western blot - Anti-Ifi204 antibody [EPR25256-211] (ab307201)
Blocking and diluting buffer and concentration: 5% NFDM/TBST

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

Exposure time: 26 seconds.
All lanes: Western blot - Anti-Ifi204 antibody [EPR25256-211] (ab307201) at 1/1000 dilution
All lanes: NIH/3T3 whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 82 kDa
Exposure time: 26s
Immunohistochemistry (Frozen sections) - Anti-Ifi204 antibody [EPR25256-211] (ab307201)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen (fresh) tissue labeling Ifi204 with ab307201 at 1/100 dilution (4.77 μg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/mL) (Green).

Confocal image showing positive staining on mouse spleen.

The section was incubated with ab307201 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/mL).
Immunohistochemistry - Anti-Ifi204 antibody [EPR25256-211] (ab307201)
Immunohistochemical analysis of paraffin-embedded mouse glioblastoma tissue labeling Ifi204 with ab307201 at 1/2000 dilution (0.239 μg/ml) followed by ready to use Leica DS9800 (Bond® Polymer Refine Detection) kit.

Nuclear staining on mouse glioblastoma.

The section was incubated with ab307201 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Leica DS9800 (Bond® Polymer Refine Detection) kit.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.
Immunohistochemistry - Anti-Ifi204 antibody [EPR25256-211] (ab307201)
Immunohistochemical analysis of paraffin-embedded mouse lung cancer tissue labeling Ifi204 with ab307201 at 1/2000 dilution (0.239 μg/ml) followed by ready to use Leica DS9800 (Bond® Polymer Refine Detection) kit.

Nuclear staining on mouse lung cancer.

The section was incubated with ab307201 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Leica DS9800 (Bond® Polymer Refine Detection) kit.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-Ifi204 antibody [EPR25256-211] (ab307201)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MEF (mouse embryo fibroblast) cells labeling Ifi204 with ab307201 at 1/50 dilution (9.54 μg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 μg/ml) (Green).

Confocal image showing mainly nuclear staining in MEF cells.

The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/ml) (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/ml).
Immunohistochemistry - Anti-Ifi204 antibody [EPR25256-211] (ab307201)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Ifi204 with ab307201 at 1/2000 dilution (0.239 μg/ml) followed by ready to use Leica DS9800 (Bond® Polymer Refine Detection) kit.

Nuclear staining on mouse spleen.

The section was incubated with ab307201 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Leica DS9800 (Bond® Polymer Refine Detection) kit.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.