Anti-Ifi204 antibody [EPR25256-211] - BSA and Azide free

• IHC

Supplier Data

Immunohistochemistry - Anti-Ifi204 antibody [EPR25256-211] - BSA and Azide free (AB307202)

This data was developed using ab307201, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse glioblastoma tissue labeling Ifi204 with ab307201 at 1/2000 dilution (0.239 μg/ml) followed by ready to use Leica DS9800 (Bond® Polymer Refine Detection) kit. Nuclear staining on mouse glioblastoma. The section was incubated with ab307201 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use Leica DS9800 (Bond® Polymer Refine Detection) kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-Ifi204 antibody [EPR25256-211] - BSA and Azide free (AB307202)

This data was developed using ab307201, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Ifi204 with ab307201 at 1/2000 dilution (0.239 μg/ml) followed by ready to use Leica DS9800 (Bond® Polymer Refine Detection) kit. Nuclear staining on mouse spleen. The section was incubated with ab307201 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use Leica DS9800 (Bond® Polymer Refine Detection) kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-Ifi204 antibody [EPR25256-211] - BSA and Azide free (AB307202)

This data was developed using ab307201, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse lung cancer tissue labeling Ifi204 with ab307201 at 1/2000 dilution (0.239 μg/ml) followed by ready to use Leica DS9800 (Bond® Polymer Refine Detection) kit. Nuclear staining on mouse lung cancer. The section was incubated with ab307201 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use Leica DS9800 (Bond® Polymer Refine Detection) kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Ifi204 antibody [EPR25256-211] - BSA and Azide free (AB307202)

This data was developed using ab307201, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MEF (mouse embryo fibroblast) cells labeling Ifi204 with ab307201 at 1/50 dilution (9.54 μg/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 μg/ml) (Green). Confocal image showing mainly nuclear staining in MEF cells. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/ml).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Ifi204 antibody [EPR25256-211] - BSA and Azide free (AB307202)

This data was developed using ab307201, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MEF (mouse embryonic fibroblast (immortalized)) cells labeling Ifi204 with ab307201 at 1/50 dilution (1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Ifi204 antibody [EPR25256-211] - BSA and Azide free (AB307202)

This data was developed using ab307201, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen (fresh) tissue labeling Ifi204 with ab307201 at 1/100 dilution (4.77 μg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing positive staining on mouse spleen. The section was incubated with ab307201 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/mL).

• WB

Supplier Data

Western blot - Anti-Ifi204 antibody [EPR25256-211] - BSA and Azide free (AB307202)

This data was developed using ab307201, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : 26 seconds.

All lanes:

Western blot - Anti-Ifi204 antibody [EPR25256-211] (<a href='/en-us/products/primary-antibodies/ifi204-antibody-epr25256-211-ab307201'>ab307201</a>) at 1/1000 dilution

All lanes:

NIH/3T3 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 82 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-Ifi204 antibody [EPR25256-211] - BSA and Azide free (AB307202)

This data was developed using ab307201, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : 59 seconds

All lanes:

Western blot - Anti-Ifi204 antibody [EPR25256-211] (<a href='/en-us/products/primary-antibodies/ifi204-antibody-epr25256-211-ab307201'>ab307201</a>) at 1/1000 dilution

Lane 1:

MEF transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

MEF transfected with siRNA specifically targeting Ifi204 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 82 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-Ifi204 antibody [EPR25256-211] - BSA and Azide free (AB307202)

This data was developed using ab307201, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : Lane 1 : 180 seconds, Lane 2 : 70 seconds.

All lanes:

Western blot - Anti-Ifi204 antibody [EPR25256-211] (<a href='/en-us/products/primary-antibodies/ifi204-antibody-epr25256-211-ab307201'>ab307201</a>) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 82 kDa

false

Exposure time: 180s