Mouse Monoclonal IFI35 antibody. Suitable for WB and reacts with Recombinant fragment samples. Immunogen corresponding to Recombinant Fragment Protein within Human IFI35.
Images
![Western blot - Anti-IFI35 antibody [2461C3a] (AB74416), expandable thumbnail](https://content.abcam.com/products/images/ifi35-antibody-2461c3a-ab74416--western-blot-img21947.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IFI35 antibody [2461C3a] (AB74416)")
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride, 0.0204% Potassium phosphate monobasic- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human IFI35. The exact immunogen used to generate this antibody is proprietary information. Database link P80217
Reactivity data
Select an application| WB | |
|---|---|
| Recombinant fragment | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Acts as a signaling pathway regulator involved in innate immune system response (PubMed:26342464, PubMed:29038465, PubMed:29350881). In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator NMI to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation (PubMed:10779520, PubMed:10950963). In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production (PubMed:26342464). In complex with NMI, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries (PubMed:29350881). Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation when actively released by macrophage to the extracellular space during cell injury and pathogen invasion (PubMed:29038465). Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of proinflammatory cytokines (PubMed:29038465).
Alternative names
Interferon-induced 35 kDa protein, IFP 35, Ifi-35, IFI35, IFP35
Recommended products
Mouse Monoclonal IFI35 antibody. Suitable for WB and reacts with Recombinant fragment samples. Immunogen corresponding to Recombinant Fragment Protein within Human IFI35.
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride, 0.0204% Potassium phosphate monobasic- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human IFI35. The exact immunogen used to generate this antibody is proprietary information. Database link P80217
- Clone number
2461C3a
- Purification technique
Affinity purification Protein G
- Concentration
- Purification notes
ab 74416 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IFI35 also known as Interferon-induced 35 kDa protein plays a role mechanically in the regulation of immune responses. It has a molecular mass of approximately 35 kDa. This protein is expressed in various tissues with high levels found in the liver lungs and spleen. IFI35 interacts with numerous proteins particularly within the cytoplasm to execute its functions.
- Biological function summary
The protein modulates the innate immune system and interacts with other interferon-induced proteins. It forms a complex with NMI (N-Myc interactor) which stabilizes each other. Through these interactions IFI35 contributes to antiviral defense mechanisms and possibly acts in response to cellular stress.
- Pathways
IFI35 engages in the interferon signaling pathway and the Jak-STAT pathway. These pathways play critical roles in mediating immune responses to viral infections. IFI35 works together with related proteins like STAT1 and NMI within these pathways providing a framework for antiviral activity and influence on cell survival.
- Associated diseases and disorders
IFI35 has associations with viral infections and autoimmune diseases. Viral pathogenesis can see modulation by IFI35 particularly infections caused by the hepatitis C virus. Furthermore its interaction with NMI may also play a part in autoimmune disorders by influencing inflammatory responses. These links highlight IFI35's significant contributions to disease mechanisms and therapeutic potential in managing immune-related conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Western blot - Anti-IFI35 antibody [2461C3a] (ab74416), expandable thumbnail](https://content.abcam.com/products/images/ifi35-antibody-2461c3a-ab74416--western-blot-img21947.jpg "Western blot - Anti-IFI35 antibody [2461C3a] (ab74416)")
Western blot - Anti-IFI35 antibody [2461C3a] (ab74416)
All lanes: Western blot - Anti-IFI35 antibody [2461C3a] (ab74416)
All lanes: immunising recombinant fragment
Predicted band size: 32 kDa
Observed band size: 32 kDa
Downloads
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