AB233415

Anti-IFI35 antibody

Name: Anti-IFI35 antibody (ab233415) | Abcam
Brand: Abcam Limited
SKU: AB233415

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(5 Publications)

Rabbit Polyclonal IFI35 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Recombinant full length protein - Human, Pig samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IFI35 aa 1-300.

View Alternative Names

IFP35, IFI35, Interferon-induced 35 kDa protein, IFP 35, Ifi-35

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI35 antibody (AB233415)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI35 antibody (AB233415)

Formalin-fixed, paraffin-embedded human liver tissue stained for IFI35 using ab233415 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Immunocytochemistry/ Immunofluorescence - Anti-IFI35 antibody (AB233415)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IFI35 antibody (AB233415)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for IFI35 (green) using ab233415 at 20 μg/ml in ICC/IF. FITC staining.

Supplier Data

Western blot - Anti-IFI35 antibody (AB233415)

All lanes:

Western blot - Anti-IFI35 antibody (ab233415) at 2 µg/mL

Lane 1:

Human lung lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 3:

Pig liver lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 32 kDa

false

Unknown

Western blot - Anti-IFI35 antibody (AB233415)

All lanes:

Western blot - Anti-IFI35 antibody (ab233415) at 2 µg/mL

All lanes:

Recombinant human IFI35 protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human IFI35 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P80217

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-20 µg/mL", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IFI35 also known as Interferon-induced 35 kDa protein plays a role mechanically in the regulation of immune responses. It has a molecular mass of approximately 35 kDa. This protein is expressed in various tissues with high levels found in the liver lungs and spleen. IFI35 interacts with numerous proteins particularly within the cytoplasm to execute its functions.

Biological function summary

The protein modulates the innate immune system and interacts with other interferon-induced proteins. It forms a complex with NMI (N-Myc interactor) which stabilizes each other. Through these interactions IFI35 contributes to antiviral defense mechanisms and possibly acts in response to cellular stress.

Pathways

IFI35 engages in the interferon signaling pathway and the Jak-STAT pathway. These pathways play critical roles in mediating immune responses to viral infections. IFI35 works together with related proteins like STAT1 and NMI within these pathways providing a framework for antiviral activity and influence on cell survival.

IFI35 has associations with viral infections and autoimmune diseases. Viral pathogenesis can see modulation by IFI35 particularly infections caused by the hepatitis C virus. Furthermore its interaction with NMI may also play a part in autoimmune disorders by influencing inflammatory responses. These links highlight IFI35's significant contributions to disease mechanisms and therapeutic potential in managing immune-related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Acts as a signaling pathway regulator involved in innate immune system response (PubMed : 26342464, PubMed : 29038465, PubMed : 29350881). In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator NMI to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation (PubMed : 10779520, PubMed : 10950963). In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production (PubMed : 26342464). In complex with NMI, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries (PubMed : 29350881). Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation when actively released by macrophage to the extracellular space during cell injury and pathogen invasion (PubMed : 29038465). Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of pro-inflammatory cytokines (PubMed : 29038465).

See full target information IFI35

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 31:738-752 PubMed38594444

2024

IFI35 regulates non-canonical NF-κB signaling to maintain glioblastoma stem cells and recruit tumor-associated macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Daqi Li,Xiefeng Wang,Kexin Chen,Danyang Shan,Gaoyuan Cui,Wei Yuan,Qiankun Lin,Ryan C Gimple,Deobrat Dixit,Chenfei Lu,Danling Gu,Hao You,Jiancheng Gao,Yangqing Li,Tao Kang,Junlei Yang,Hang Yu,Kefan Song,Zhumei Shi,Xiao Fan,Qiulian Wu,Wei Gao,Zhe Zhu,Jianghong Man,Qianghu Wang,Fan Lin,Weiwei Tao,Stephen C Mack,Yun Chen,Junxia Zhang,Chaojun Li,Nu Zhang,Yongping You,Xu Qian,Kailin Yang,Jeremy N Rich,Qian Zhang,Xiuxing Wang

Frontiers in immunology 13:923481 PubMed35844580

2022

Mapping the Tumor Microenvironment in TNBC and Deep Exploration for M1 Macrophages-Associated Prognostic Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Baojin Xu,Hefen Sun,Xiaoqing Song,Qiqi Liu,Wei Jin

Cancer cell international 21:290 PubMed34082779

2021

IFI35 is involved in the regulation of the radiosensitivity of colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Hu,Bing Wang,Ke Yi,Qingjun Lei,Guanghui Wang,Xiaohui Xu

International journal of molecular medicine 47: PubMed33907823

2021

IRF1 regulates the progression of colorectal cancer via interferon‑induced proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Xu,Yong Wu,Ke Yi,Yan Hu,Weiqun Ding,Chungen Xing

Cancer cell international 19:102 PubMed31015802

2019

Tre2 (USP6NL) promotes colorectal cancer cell proliferation via Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Sun,Song-Bing He,Yi-Zhou Yao,Jian-Guo Qu,Rong Xie,Yu-Qiao Ma,Ming-Hui Zong,Ji-Xiang Chen

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-IFI35 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI35 antibody (AB233415)

Immunocytochemistry/ Immunofluorescence - Anti-IFI35 antibody (AB233415)

Western blot - Anti-IFI35 antibody (AB233415)

Western blot - Anti-IFI35 antibody (AB233415)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise