Rabbit Polyclonal IFI35 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Recombinant full length protein - Human, Pig samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IFI35 aa 1 to C-terminus.
Images
Publications
- International journal of molecular medicine 47:2021IRF1 regulates the progression of colorectal cancer via interferon‑induced proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaohui Xu,Yong Wu,Ke Yi,Yan Hu,Weiqun Ding,Chungen XingPubMed 33907823
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human IFI35 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P80217
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Pig | Tested | Expected | Expected |
| Recombinant full length protein - Human | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.2-2 µg/mL | Notes - |
Species Recombinant full length protein - Human | Dilution info 0.2-2 µg/mL | Notes - |
Species Pig | Dilution info 0.2-2 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Acts as a signaling pathway regulator involved in innate immune system response (PubMed:26342464, PubMed:29038465, PubMed:29350881). In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator NMI to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation (PubMed:10779520, PubMed:10950963). In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production (PubMed:26342464). In complex with NMI, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries (PubMed:29350881). Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation when actively released by macrophage to the extracellular space during cell injury and pathogen invasion (PubMed:29038465). Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of proinflammatory cytokines (PubMed:29038465).
Alternative names
Interferon-induced 35 kDa protein, IFP 35, Ifi-35, IFI35, IFP35
Recommended products
Rabbit Polyclonal IFI35 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Recombinant full length protein - Human, Pig samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IFI35 aa 1 to C-terminus.
Alternative names
Interferon-induced 35 kDa protein, IFP 35, Ifi-35, IFI35, IFP35
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human IFI35 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P80217
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
IFI35 also known as Interferon-induced 35 kDa protein plays a role mechanically in the regulation of immune responses. It has a molecular mass of approximately 35 kDa. This protein is expressed in various tissues with high levels found in the liver lungs and spleen. IFI35 interacts with numerous proteins particularly within the cytoplasm to execute its functions.
- Biological function summary
The protein modulates the innate immune system and interacts with other interferon-induced proteins. It forms a complex with NMI (N-Myc interactor) which stabilizes each other. Through these interactions IFI35 contributes to antiviral defense mechanisms and possibly acts in response to cellular stress.
- Pathways
IFI35 engages in the interferon signaling pathway and the Jak-STAT pathway. These pathways play critical roles in mediating immune responses to viral infections. IFI35 works together with related proteins like STAT1 and NMI within these pathways providing a framework for antiviral activity and influence on cell survival.
- Associated diseases and disorders
IFI35 has associations with viral infections and autoimmune diseases. Viral pathogenesis can see modulation by IFI35 particularly infections caused by the hepatitis C virus. Furthermore its interaction with NMI may also play a part in autoimmune disorders by influencing inflammatory responses. These links highlight IFI35's significant contributions to disease mechanisms and therapeutic potential in managing immune-related conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Western blot - Anti-IFI35 antibody (ab233415)
All lanes: Western blot - Anti-IFI35 antibody (ab233415) at 2 µg/mL
All lanes: Recombinant human IFI35 protein
SecondaryAll lanes: HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 32 kDa
Western blot - Anti-IFI35 antibody (ab233415)
All lanes: Western blot - Anti-IFI35 antibody (ab233415) at 2 µg/mL
Lane 1: Human lung lysate
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3: Pig liver lysate
SecondaryAll lanes: HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI35 antibody (ab233415)
Formalin-fixed, paraffin-embedded human liver tissue stained for IFI35 using ab233415 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Immunocytochemistry/ Immunofluorescence - Anti-IFI35 antibody (ab233415)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for IFI35 (green) using ab233415 at 20 μg/ml in ICC/IF. FITC staining.
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