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AB70023

Anti-IFIT1 antibody

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(4 Publications)

Mouse Polyclonal IFIT1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IFIT1.

View Alternative Names

G10P1, IFI56, IFNAI1, ISG56, IFIT1, Interferon-induced protein with tetratricopeptide repeats 1, IFIT-1, Interferon-induced 56 kDa protein, IFI-56K, P56

1 Images
Western blot - Anti-IFIT1 antibody (AB70023)
  • WB

Unknown

Western blot - Anti-IFIT1 antibody (AB70023)

All lanes:

Western blot - Anti-IFIT1 antibody (ab70023) at 1/500 dilution

Lane 1:

IFIT1 transfected 293T lysate at 25 µg

Lane 2:

Non transfected 293T lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 55 kDa

Observed band size: 56 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IFIT1.

P09914

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IFIT1 also known as Interferon-Induced Protein with Tetratricopeptide Repeats 1 is a protein that plays a significant role in antiviral defense mechanisms. It has a molecular mass of approximately 56 kDa. Researchers find IFIT1 predominantly expressed in cells stimulated by interferons. The protein detects viral single-stranded RNA which in turn helps initiate the antiviral response. You will find IFIT1 in various cell types mainly those exposed to viral infections as it is an important part of the body's innate immune system.
Biological function summary

Activities of IFIT1 focus on inhibiting viral replication and translation processes. The protein binds to viral RNAs via its distinctive sensor domains preventing infections from progressing. IFIT1 operates often as part of a larger protein complex with other IFIT family members improving its ability to intercept and inhibit viral RNA. This concerted action provides an important line of defense against diverse viral pathogens by interfering with viral protein synthesis.

Pathways

IFIT1 occupies an important place in the interferon signaling pathways particularly the Jak-STAT pathway. This pathway is critical for mounting antiviral responses when viral infections occur. Through its interactions within the pathway IFIT1 works together with related proteins like IFIT2 and IFIT3 to repel viral threats. By integrating into these pathways it contributes to amplifying the immune response promoting a robust defense system against foreign viral invaders.

IFIT1 is associated mainly with viral diseases such as influenza and hepatitis C. Its role in these diseases involves preventing the communication and replication of the viruses making it a pivotal part of antiviral therapeutic research. Additionally proteins like RIG-I interact with IFIT1 during these processes to recognize viral RNA enhancing the effectiveness of the immune response. These protein partnerships drive ongoing research looking to leverage IFIT1's antiviral properties for treatment developments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses.
See full target information IFIT1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

mBio 12: PubMed33785626

2021

Multiple Viral microRNAs Regulate Interferon Release and Signaling Early during Infection with Epstein-Barr Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Mickaël Bouvet,Stefanie Voigt,Takanobu Tagawa,Manuel Albanese,Yen-Fu Adam Chen,Yan Chen,Devin N Fachko,Dagmar Pich,Christine Göbel,Rebecca L Skalsky,Wolfgang Hammerschmidt

PLoS pathogens 12:e1005955 PubMed27907166

2016

Vaccinia Virus Protein C6 Inhibits Type I IFN Signalling in the Nucleus and Binds to the Transactivation Domain of STAT2.

Applications

Flow Cyt

Species

Unspecified reactive species

Jennifer H Stuart,Rebecca P Sumner,Yongxu Lu,Joseph S Snowden,Geoffrey L Smith

Oncogene 31:1242-53 PubMed21804607

2011

Absent in Melanoma 2 (AIM2) is an important mediator of interferon-dependent and -independent HLA-DRA and HLA-DRB gene expression in colorectal cancers.

Applications

Unspecified application

Species

Human

J Lee,L Li,N Gretz,J Gebert,S Dihlmann

Cytokine 53:52-9 PubMed20943413

2010

The differential activity of interferon-α subtypes is consistent among distinct target genes and cell types.

Applications

WB

Species

Human

Herwig P Moll,Thomas Maier,Anna Zommer,Thomas Lavoie,Christine Brostjan
View all publications
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