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Rabbit Recombinant Monoclonal IFIT1 antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Human samples.

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Images

Immunoprecipitation - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (AB305302), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (AB305302), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (AB305302), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (AB305302), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (AB305302), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses.

Alternative names

G10P1, IFI56, IFNAI1, ISG56, IFIT1, Interferon-induced protein with tetratricopeptide repeats 1, IFIT-1, Interferon-induced 56 kDa protein, IFI-56K, P56

Recommended products

Rabbit Recombinant Monoclonal IFIT1 antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27276-63
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

IFIT1 also known as Interferon-Induced Protein with Tetratricopeptide Repeats 1 is a protein that plays a significant role in antiviral defense mechanisms. It has a molecular mass of approximately 56 kDa. Researchers find IFIT1 predominantly expressed in cells stimulated by interferons. The protein detects viral single-stranded RNA which in turn helps initiate the antiviral response. You will find IFIT1 in various cell types mainly those exposed to viral infections as it is an important part of the body's innate immune system.

Biological function summary

Activities of IFIT1 focus on inhibiting viral replication and translation processes. The protein binds to viral RNAs via its distinctive sensor domains preventing infections from progressing. IFIT1 operates often as part of a larger protein complex with other IFIT family members improving its ability to intercept and inhibit viral RNA. This concerted action provides an important line of defense against diverse viral pathogens by interfering with viral protein synthesis.

Pathways

IFIT1 occupies an important place in the interferon signaling pathways particularly the Jak-STAT pathway. This pathway is critical for mounting antiviral responses when viral infections occur. Through its interactions within the pathway IFIT1 works together with related proteins like IFIT2 and IFIT3 to repel viral threats. By integrating into these pathways it contributes to amplifying the immune response promoting a robust defense system against foreign viral invaders.

Associated diseases and disorders

IFIT1 is associated mainly with viral diseases such as influenza and hepatitis C. Its role in these diseases involves preventing the communication and replication of the viruses making it a pivotal part of antiviral therapeutic research. Additionally proteins like RIG-I interact with IFIT1 during these processes to recognize viral RNA enhancing the effectiveness of the immune response. These protein partnerships drive ongoing research looking to leverage IFIT1's antiviral properties for treatment developments.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunoprecipitation - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (ab305302), expandable thumbnail

    Immunoprecipitation - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (ab305302)

    This data was developed using Anti-IFIT1 antibody [EPR27276-63] ab305301, the same antibody clone in a different buffer formulation.
    IFIT1 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) treated with 10ng/ml IFN alpha 1 for 16h whole cell lysate 10 ug with Anti-IFIT1 antibody [EPR27276-63] ab305301 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-IFIT1 antibody [EPR27276-63] ab305301 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa treated with 10ng/ml IFN alpha 1 for 16h whole cell lysate 10 ug

    Lane 2: Anti-IFIT1 antibody [EPR27276-63] ab305301 IP in HeLa treated with 10ng/ml IFN alpha 1 for 16h whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IFIT1 antibody [EPR27276-63] ab305301 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 41 seconds.

    All lanes: Immunoprecipitation - Anti-IFIT1 antibody [EPR27276-63] (Anti-IFIT1 antibody [EPR27276-63] ab305301) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) treated with 10ng/ml IFN alpha 1 for 16h whole cell lysate 10 µg

    Lane 2: HeLa treated with 10ng/ml IFN alpha 1 for 16h whole cell lysate

    Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 56 kDa

    Exposure time: 41s

  • Flow Cytometry (Intracellular) - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (ab305302), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (ab305302)

    This data was developed using Anti-IFIT1 antibody [EPR27276-63] ab305301, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) treated with 10ng/ml human IFN alpha 1 for 16 hours (Red) / Untreated control (Green) cells labeling IFIT1 with Anti-IFIT1 antibody [EPR27276-63] ab305301 at 1/500 dilution (0.1ug) (Red) and Green (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (ab305302), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (ab305302)

    This data was developed using Anti-IFIT1 antibody [EPR27276-63] ab305301, the same antibody clone in a different buffer formulation.
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labeling IFIT1 with Anti-IFIT1 antibody [EPR27276-63] ab305301 at 1/50 (10.44 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing weak cytoplasmic staining in HeLa cell line, and the expression increased after treatment with IFN alpha 1 (10 ng/ml) for 16 hours.

    The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (ab305302), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (ab305302)

    This data was developed using Anti-IFIT1 antibody [EPR27276-63] ab305301, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded HeLa cell pellet labeling IFIT1 with Anti-IFIT1 antibody [EPR27276-63] ab305301 at 1/2000 (0.261 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Cytoplasmic staining on HeLa cell pellet treated with IFN-alpha 1(10ng/ml) for 16 hours, no staining on HeLa cell pellet (untreated).

    The section was incubated with Anti-IFIT1 antibody [EPR27276-63] ab305301 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (ab305302), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (ab305302)

    This data was developed using Anti-IFIT1 antibody [EPR27276-63] ab305301, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling IFIT1 with Anti-IFIT1 antibody [EPR27276-63] ab305301 at 1/2000 (0.261 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Cytoplasmic staining on human breast cancer (PMID: 23528130).

    The section was incubated with Anti-IFIT1 antibody [EPR27276-63] ab305301 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (ab305302), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (ab305302)

    This data was developed using Anti-IFIT1 antibody [EPR27276-63] ab305301, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling IFIT1 with Anti-IFIT1 antibody [EPR27276-63] ab305301 at 1/2000 (0.261 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Cytoplasmic staining on human lung carcinoma (PMID: 35280763).

    The section was incubated with Anti-IFIT1 antibody [EPR27276-63] ab305301 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (ab305302), expandable thumbnail

    Western blot - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (ab305302)

    This data was developed using Anti-IFIT1 antibody [EPR27276-63] ab305301, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST  

    Exposure time: 26 seconds

    All lanes: Western blot - Anti-IFIT1 antibody [EPR27276-63] (Anti-IFIT1 antibody [EPR27276-63] ab305301) at 1/1000 dilution

    Lane 1: Human tonsil tissue lysate 40 µg

    Lane 2: Human testis tissue lysate 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 56 kDa

    Exposure time: 26s

  • Western blot - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (ab305302), expandable thumbnail

    Western blot - Anti-IFIT1 antibody [EPR27276-63] (BSA and Azide free) (ab305302)

    This data was developed using Anti-IFIT1 antibody [EPR27276-63] ab305301, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST  

    Exposure time: 180 seconds

    All lanes: Western blot - Anti-IFIT1 antibody [EPR27276-63] (Anti-IFIT1 antibody [EPR27276-63] ab305301) at 1/1000 dilution

    Lane 1: Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 20 µg

    Lane 2: HeLa treated with /ml IFN alpha 1 for 16h whole cell lysate 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 56 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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