Anti-IFIT2 antibody

• WB

Unknown

Western blot - Anti-IFIT2 antibody (AB113112)

All lanes:

Western blot - Anti-IFIT2 antibody (ab113112) at 1/1000 dilution

Lane 1:

Human fetal liver lysate

Lane 2:

Human fetal lung lysate

Predicted band size: 55 kDa

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• WB

CiteAb

Western blot - Anti-IFIT2 antibody (AB113112)

IFIT2 western blot using anti-IFIT2 antibody ab113112. Publication image and figure legend from Pinkham, C., Dahal, B., et al., 2017, Sci Rep, PubMed 29085037.

ab113112 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab113112 please see the product overview.

Increases in host cellular mRNA does not correlate with increases in protein expression. (a) HSAECs were mock-infected or infected with MP12 for one hour. At 18 hours post infection, cell lysates were collected and analyzed by western blot. As a control, U937 cell lysates were used. At 24 hours post infection, cell lysates were collected and analyzed in parallel by western blot. Membranes were probed for levels of ARHGEF6 (α-PIX) and β-Actin as a loading control. (b) HSAECs were mock-infected, infected with MP12, or infected with MP12-ΔNSs for one hour. Following infection, cells were treated with Actinomycin D (1 µg/mL) for the remaining time. At 18 hours post infection, cell lysates were collected and analyzed in parallel by western blot for levels of IFIT2, Viperin, α-Actinin-2, and β-Actin as a loading control. Cropped, representative images are shown here. Uncropped western blots can be found in Supplemental Fig. 8.

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