JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB168407

Anti-IFIT2 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Mouse Polyclonal IFIT2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IFIT2.

View Alternative Names

CIG-42, G10P2, IFI54, ISG54, IFIT2, Interferon-induced protein with tetratricopeptide repeats 2, IFIT-2, ISG-54 K, Interferon-induced 54 kDa protein, IFI-54K, P54

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-IFIT2 antibody (AB168407)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IFIT2 antibody (AB168407)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling IFIT2 using ab168407 at 10μg/ml.

Western blot - Anti-IFIT2 antibody (AB168407)
  • WB

Unknown

Western blot - Anti-IFIT2 antibody (AB168407)

All lanes:

Western blot - Anti-IFIT2 antibody (ab168407) at 1 µg/mL

Lane 1:

IFIT2 transfected 293T cell line lysate

Lane 2:

Non-transfected 293T cell line lysate

Predicted band size: 55 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IFIT2.

P09913

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IFIT2 also known as Interferon-Induced Protein with Tetratricopeptide Repeats 2 functions as a critical player in antiviral defense mechanisms. This protein possesses a molecular mass of approximately 54 kDa. IFIT2 expression occurs mostly in cells that respond to type I interferons and during viral infections. The protein locates within the cytoplasm where it acts by recognizing non-self RNA aiding in the inhibition of viral replication.
Biological function summary

The protein exerts its influence by binding to RNA molecules lacking 2'-O-methylation a modification that is usually absent in viral RNA. IFIT2 works as both a solo entity and as part of a larger complex that includes other members like IFIT1 and IFIT3. The coordination among these proteins helps modulate the immune response influencing protein synthesis regulation in cells that are under viral attack.

Pathways

IFIT2 plays an essential role in the innate immune response particularly the interferon signaling pathway. It contributes to this pathway through its interactions with other proteins ensuring the rapid activation of antiviral states. Furthermore IFIT2 also connects to the toll-like receptor (TLR) signaling pathway playing a part in recognizing viral components. Through these pathways IFIT2 typically associates with proteins like TLR3 which facilitate detection and response to viral pathogens.

Chronic viral infections and certain types of cancer show a significant connection with IFIT2. In viral diseases reduced or impaired IFIT2 activity can lead to increased viral replication exacerbating the infection. This protein also gets involved in mechanisms affecting cancer progression often in connection with pathways involving the protein p53 a well-known tumor suppressor. The interaction between IFIT2 and these disease-related pathways highlights its potential as a target for therapeutic development.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.
See full target information IFIT2
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107783 PubMed39303913

2024

Downregulation of the mA reader YTHDC2 upregulates exosome content in lung adenocarcinoma via inhibiting IFIT and OAS family members.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Yin,Lifang Ma,Xiaoting Tian,Qi Sun,Congcong Zhang,Yikun Wang,Yayou Miao,Xiangfei Xue,Yongjie Wang,Jiayi Wang,Xiao Zhang,Xumin Hou

Journal of cellular and molecular medicine 25:1274-1289 PubMed33325143

2020

T and B cell Epitope analysis of SARS-CoV-2 S protein based on immunoinformatics and experimental research.

Applications

Unspecified application

Species

Unspecified reactive species

Ziwei Chen,Pinglang Ruan,Lili Wang,Xinmin Nie,Xuejun Ma,Yurong Tan
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB154487

Anti-IFIT2 antibody - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-IFIT2 antibody - N-terminal (AB154487)

  • 0
  • 0
Primary Antibodies

AB113112

Anti-IFIT2 antibody

Western blot - Anti-IFIT2 antibody (AB113112)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB76818

Anti-IFIT3/P60 antibody

Western blot - Anti-IFIT3/P60 antibody (AB76818)

  • 0
  • 0
Primary Antibodies

AB191871

Anti-REDD-1/DDIT4 antibody [EPR18716]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-REDD-1/DDIT4 antibody [EPR18716] (AB191871)

  • 5
  • 1
Primary Antibodies

AB175239

Anti-MVP antibody [EPR13227(B)]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] (AB175239)

  • 0
  • 0
Primary Antibodies

AB171085

Anti-IGFBP7 antibody [EPR11912(B)]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP7 antibody [EPR11912(B)] (AB171085)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com