Anti-IFIT2 antibody [EPR26404-119]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] (AB305231)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling IFIT2 with ab305231 at 1/500 (1.006 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Negative control : no staining on human skeletal muscle. The section was incubated with ab305231 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] (AB305231)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling IFIT2 with ab305231 at 1/500 (1.006 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human cervical carcinoma. The section was incubated with ab305231 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] (AB305231)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling IFIT2 with ab305231 at 1/500 (1.006 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human kidney. The section was incubated with ab305231 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] (AB305231)

Immunohistochemical analysis of paraffin-embedded A, A431 (Human epidermoid tissue labeling IFIT2 with ab305231 at 1/500 (1.006 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on A431 cell pellet and almost no staining on HCT 116 cell pellet. The section was incubated with ab305231 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-IFIT2 antibody [EPR26404-119] (AB305231)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling IFIT2 with ab305231 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• IP

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Immunoprecipitation - Anti-IFIT2 antibody [EPR26404-119] (AB305231)

IFIT2 was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell) whole cell lysate 10 μg with ab305231 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305231 at dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : A431 (human epidermoid carcinoma epithelial cell) whole cell lysate 10 μg Lane 2 : ab305231 IP in A431 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305231 in A431 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-IFIT2 antibody [EPR26404-119] (ab305231) at 1/30 dilution

All lanes:

A431 (human epidermoid carcinoma epithelial cell)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 55 kDa

false

Exposure time: 3min

• WB

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Western blot - Anti-IFIT2 antibody [EPR26404-119] (AB305231)

Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human IFIT1, IFIT3, or IFIT4. Exposure time : 8 seconds

All lanes:

Western blot - Anti-IFIT2 antibody [EPR26404-119] (ab305231) at 1/1000 dilution

Lane 1:

HEK-293T transfected with human IFIT1 expression vector containi a myc-His-tag®, whole cell lysate at 40 µg

Lane 2:

HEK-293T transfected with human IFIT2 expression vector containi a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

HEK-293T transfected with human IFIT3 expression vector containi a myc-His-tag®, whole cell lysate 20 at 20 µg

Lane 4:

HEK-293T transfected with human IFIT4 expression vector containi a myc-His-tag®, whole cell lysate 20 at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55 kDa

false

Exposure time: 8s

• WB

Supplier Data

Western blot - Anti-IFIT2 antibody [EPR26404-119] (AB305231)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : HCT116 (PMID : 29245969). Exposure time : 3 minutes

All lanes:

Western blot - Anti-IFIT2 antibody [EPR26404-119] (ab305231) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human lu tissue lysate at 20 µg

Lane 3:

Human kidney tissue lysate at 20 µg

Lane 4:

A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 5:

HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55 kDa

false

Exposure time: 3min