Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (AB305232)

This data was developed using ab305231, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human skeletal muscl tissue labeling IFIT2 with ab305231 at 1/500 (1.006 µg/ml) followed by a ready to use Leica DS9800 (Bond™, Polymer Refine Detection) kit. Negative control : no staining on human skeletal muscle. The section was incubated with ab305231 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (Bond™, Polymer Refine Detection) kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (AB305232)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (AB305232)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (AB305232)

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (AB305232)

This data was developed using ab305231, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling IFIT2 with ab305231 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• IP

Supplier Data

Immunoprecipitation - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (AB305232)

This data was developed using ab305231, the same antibody clone in a different buffer formulation. IFIT2 was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell) whole cell lysate 10 μg with ab305231 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305231. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : A431 (human epidermoid carcinoma epithelial cell) whole cell lysate 10 μg Lane 2 : ab305231 IP in A431 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305231 in A431 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-IFIT2 antibody [EPR26404-119] (<a href='/en-us/products/primary-antibodies/ifit2-antibody-epr26404-119-ab305231'>ab305231</a>) at 1/30 dilution

All lanes:

A431 (human epidermoid carcinoma epithelial cell)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 55 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (AB305232)

This data was developed using ab305231, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human IFIT1, IFIT3, or IFIT4. Exposure time : 8 seconds

All lanes:

Western blot - Anti-IFIT2 antibody [EPR26404-119] (<a href='/en-us/products/primary-antibodies/ifit2-antibody-epr26404-119-ab305231'>ab305231</a>) at 1/1000 dilution

Lane 1:

HEK-293T transfected with human IFIT1 expression vector containi a myc-His-tag®, whole cell lysate at 40 µg

Lane 2:

HEK-293T transfected with human IFIT2 expression vector containi a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

HEK-293T transfected with human IFIT3 expression vector containi a myc-His-tag®, whole cell lysate 20 at 20 µg

Lane 4:

HEK-293T transfected with human IFIT4 expression vector containi a myc-His-tag®, whole cell lysate 20 at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55 kDa

false

Exposure time: 8s

• WB

Supplier Data

Western blot - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (AB305232)

This data was developed using ab305231, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : HCT116 (PMID : 29245969). Exposure time : 3 minutes

All lanes:

Western blot - Anti-IFIT2 antibody [EPR26404-119] (<a href='/en-us/products/primary-antibodies/ifit2-antibody-epr26404-119-ab305231'>ab305231</a>) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human lu tissue lysate at 20 µg

Lane 3:

Human kidney tissue lysate at 20 µg

Lane 4:

A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 5:

HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55 kDa

false

Exposure time: 3min