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Rabbit Recombinant Monoclonal IFIT2 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (AB305232), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (AB305232), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (AB305232), expandable thumbnail
  • Immunoprecipitation - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (AB305232), expandable thumbnail
  • Western blot - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (AB305232), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Tested
Tested
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.

Alternative names

CIG-42, G10P2, IFI54, ISG54, IFIT2, Interferon-induced protein with tetratricopeptide repeats 2, IFIT-2, ISG-54 K, Interferon-induced 54 kDa protein, IFI-54K, P54

Recommended products

Rabbit Recombinant Monoclonal IFIT2 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26404-119
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

IFIT2 also known as Interferon-Induced Protein with Tetratricopeptide Repeats 2 functions as a critical player in antiviral defense mechanisms. This protein possesses a molecular mass of approximately 54 kDa. IFIT2 expression occurs mostly in cells that respond to type I interferons and during viral infections. The protein locates within the cytoplasm where it acts by recognizing non-self RNA aiding in the inhibition of viral replication.

Biological function summary

The protein exerts its influence by binding to RNA molecules lacking 2'-O-methylation a modification that is usually absent in viral RNA. IFIT2 works as both a solo entity and as part of a larger complex that includes other members like IFIT1 and IFIT3. The coordination among these proteins helps modulate the immune response influencing protein synthesis regulation in cells that are under viral attack.

Pathways

IFIT2 plays an essential role in the innate immune response particularly the interferon signaling pathway. It contributes to this pathway through its interactions with other proteins ensuring the rapid activation of antiviral states. Furthermore IFIT2 also connects to the toll-like receptor (TLR) signaling pathway playing a part in recognizing viral components. Through these pathways IFIT2 typically associates with proteins like TLR3 which facilitate detection and response to viral pathogens.

Associated diseases and disorders

Chronic viral infections and certain types of cancer show a significant connection with IFIT2. In viral diseases reduced or impaired IFIT2 activity can lead to increased viral replication exacerbating the infection. This protein also gets involved in mechanisms affecting cancer progression often in connection with pathways involving the protein p53 a well-known tumor suppressor. The interaction between IFIT2 and these disease-related pathways highlights its potential as a target for therapeutic development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (ab305232), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (ab305232)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (ab305232), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (ab305232)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (ab305232), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (ab305232)

  • Immunoprecipitation - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (ab305232), expandable thumbnail

    Immunoprecipitation - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (ab305232)

    This data was developed using Anti-IFIT2 antibody [EPR26404-119] ab305231, the same antibody clone in a different buffer formulation.
    IFIT2 was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell) whole cell lysate 10 μg with Anti-IFIT2 antibody [EPR26404-119] ab305231 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-IFIT2 antibody [EPR26404-119] ab305231. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate 10 μg

    Lane 2: Anti-IFIT2 antibody [EPR26404-119] ab305231 IP in A431 whole cell lysate

    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IFIT2 antibody [EPR26404-119] ab305231 in A431 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

    All lanes: Immunoprecipitation - Anti-IFIT2 antibody [EPR26404-119] (Anti-IFIT2 antibody [EPR26404-119] ab305231) at 1/30 dilution

    All lanes: A431 (human epidermoid carcinoma epithelial cell)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 55 kDa

    Exposure time: 3min

  • Western blot - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (ab305232), expandable thumbnail

    Western blot - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (ab305232)

    This data was developed using Anti-IFIT2 antibody [EPR26404-119] ab305231, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: HCT116 (PMID: 29245969).
    Exposure time: 3 minutes

    All lanes: Western blot - Anti-IFIT2 antibody [EPR26404-119] (Anti-IFIT2 antibody [EPR26404-119] ab305231) at 1/1000 dilution

    Lane 1: Human liver tissue lysate at 20 µg

    Lane 2: Human lu tissue lysate at 20 µg

    Lane 3: Human kidney tissue lysate at 20 µg

    Lane 4: A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 5: HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 55 kDa

    Exposure time: 3min

  • Western blot - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (ab305232), expandable thumbnail

    Western blot - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (ab305232)

    This data was developed using Anti-IFIT2 antibody [EPR26404-119] ab305231, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody does not cross-react with human IFIT1, IFIT3, or IFIT4.
    Exposure time: 8 seconds

    All lanes: Western blot - Anti-IFIT2 antibody [EPR26404-119] (Anti-IFIT2 antibody [EPR26404-119] ab305231) at 1/1000 dilution

    Lane 1: HEK-293T transfected with human IFIT1 expression vector containi a myc-His-tag®, whole cell lysate at 40 µg

    Lane 2: HEK-293T transfected with human IFIT2 expression vector containi a myc-His-tag®, whole cell lysate at 20 µg

    Lane 3: HEK-293T transfected with human IFIT3 expression vector containi a myc-His-tag®, whole cell lysate 20 at 20 µg

    Lane 4: HEK-293T transfected with human IFIT4 expression vector containi a myc-His-tag®, whole cell lysate 20 at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 55 kDa

    Exposure time: 8s

  • Flow Cytometry (Intracellular) - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (ab305232), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IFIT2 antibody [EPR26404-119] - BSA and Azide free (ab305232)

    This data was developed using Anti-IFIT2 antibody [EPR26404-119] ab305231, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling IFIT2 with Anti-IFIT2 antibody [EPR26404-119] ab305231 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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