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AB154487

Anti-IFIT2 antibody - N-terminal

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(1 Publication)

Rabbit Polyclonal IFIT2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human IFIT2 aa 1-50.

View Alternative Names

CIG-42, G10P2, IFI54, ISG54, IFIT2, Interferon-induced protein with tetratricopeptide repeats 2, IFIT-2, ISG-54 K, Interferon-induced 54 kDa protein, IFI-54K, P54

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-IFIT2 antibody - N-terminal (AB154487)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IFIT2 antibody - N-terminal (AB154487)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling IFIT2 with ab154487 at 1/200 dilution. Lower image is merged with DNA probe.

Western blot - Anti-IFIT2 antibody - N-terminal (AB154487)
  • WB

Unknown

Western blot - Anti-IFIT2 antibody - N-terminal (AB154487)

7.5% SDS PAGE

All lanes:

Western blot - Anti-IFIT2 antibody - N-terminal (ab154487) at 1/1000 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human IFIT2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P09913

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IFIT2 also known as Interferon-Induced Protein with Tetratricopeptide Repeats 2 functions as a critical player in antiviral defense mechanisms. This protein possesses a molecular mass of approximately 54 kDa. IFIT2 expression occurs mostly in cells that respond to type I interferons and during viral infections. The protein locates within the cytoplasm where it acts by recognizing non-self RNA aiding in the inhibition of viral replication.
Biological function summary

The protein exerts its influence by binding to RNA molecules lacking 2'-O-methylation a modification that is usually absent in viral RNA. IFIT2 works as both a solo entity and as part of a larger complex that includes other members like IFIT1 and IFIT3. The coordination among these proteins helps modulate the immune response influencing protein synthesis regulation in cells that are under viral attack.

Pathways

IFIT2 plays an essential role in the innate immune response particularly the interferon signaling pathway. It contributes to this pathway through its interactions with other proteins ensuring the rapid activation of antiviral states. Furthermore IFIT2 also connects to the toll-like receptor (TLR) signaling pathway playing a part in recognizing viral components. Through these pathways IFIT2 typically associates with proteins like TLR3 which facilitate detection and response to viral pathogens.

Chronic viral infections and certain types of cancer show a significant connection with IFIT2. In viral diseases reduced or impaired IFIT2 activity can lead to increased viral replication exacerbating the infection. This protein also gets involved in mechanisms affecting cancer progression often in connection with pathways involving the protein p53 a well-known tumor suppressor. The interaction between IFIT2 and these disease-related pathways highlights its potential as a target for therapeutic development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.
See full target information IFIT2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics 29:2788-2802 PubMed32898862

2020

Gene expression profiles complement the analysis of genomic modifiers of the clinical onset of Huntington disease.

Applications

Unspecified application

Species

Unspecified reactive species

Galen E B Wright,Nicholas S Caron,Bernard Ng,Lorenzo Casal,William Casazza,Xiaohong Xu,Jolene Ooi,Mahmoud A Pouladi,Sara Mostafavi,Colin J D Ross,Michael R Hayden
View all publications
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Product promise

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