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AB317232

Anti-IFIT3 antibody [HL2468]

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Rabbit Monoclonal IFIT3/P60 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IFIT3.

View Alternative Names

CIG-49, IFI60, IFIT4, ISG60, IFIT3, Interferon-induced protein with tetratricopeptide repeats 3, IFIT-3, CIG49, ISG-60, Interferon-induced 60 kDa protein, Interferon-induced protein with tetratricopeptide repeats 4, Retinoic acid-induced gene G protein, IFI-60K, IFIT-4, P60, RIG-G

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT3 antibody [HL2468] (AB317232)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT3 antibody [HL2468] (AB317232)

ab317232 detects IFIT3 protein at mitochondria by immunohistochemical analysis. Sample : Paraffin-embedded human glioblastoma. IFIT3 stained by ab317232 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunocytochemistry/ Immunofluorescence - Anti-IFIT3 antibody [HL2468] (AB317232)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IFIT3 antibody [HL2468] (AB317232)

ab317232 detects IFIT3 protein at cytoplasm by immunofluorescent analysis. Sample : MDA-MB-231 cells were fixed in ice-cold MeOH for 5 min. Green : IFIT3 stained by ab317232 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Western blot - Anti-IFIT3 antibody [HL2468] (AB317232)
  • WB

Supplier Data

Western blot - Anti-IFIT3 antibody [HL2468] (AB317232)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317232 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-IFIT3 antibody [HL2468] (ab317232) at 1/1000 dilution

Lane 1:

MDA-MB-231 whole cell extracts at 30 µg

Lane 2:

293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-IFIT3 antibody [HL2468] (AB317232)
  • WB

Supplier Data

Western blot - Anti-IFIT3 antibody [HL2468] (AB317232)

Untreated (–) and treated (+) A549 whole cell extracts (50 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317232 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-IFIT3 antibody [HL2468] (ab317232) at 1/1000 dilution

Lane 1:

Starvation 24hr, untreated (–) A549 whole cell extracts at 50 µg

Lane 2:

Starvation 24 hr, 100 ng/mL IFN-gamma 48 hr, treated (+) A549 whole cell extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL246

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human IFIT3.

O14879

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IFIT3 also known as P60 is a member of the IFIT (Interferon-Induced Proteins with Tetratricopeptide Repeats) family of proteins. With a molecular mass of approximately 60 kDa IFIT3 is part of the antiviral response system. This protein is expressed in various tissues and is highly inducible by type I interferons. It functions to inhibit viral replication by interacting with viral RNA. The structure of IFIT3 includes tetratricopeptide repeat motifs which facilitate its role in protein-protein interactions.
Biological function summary

IFIT3 plays a major role in the immune response against viral infections. The protein forms complexes with other IFIT family members like IFIT1 and IFIT2 to enhance antiviral efficacy. IFIT3 binds to foreign RNA lacking proper cap structures blocking viral protein synthesis. The protein's actions help strengthen the innate immune system and further control the spread of viral pathogens. IFIT3 also modulates cellular functions by engaging with signaling pathways that regulate inflammation and immune defense.

Pathways

IFIT3 integrates into important signaling networks such as the interferon-mediated signal transduction pathway and the JAK-STAT signaling pathway. In these pathways IFIT3 along with related proteins like IRF3 and ISG15 helps amplify immune responses after detecting viral threats. Its involvement in these pathways ensures that antiviral states within cells are quickly established reducing viral survival and dissemination.

IFIT3 has significant implications for infection and cancer outcomes. In viral infections such as hepatitis C IFIT3 expression levels correlate with effective antiviral activity and prognosis. In cancer aberrant IFIT3 expression can influence tumorigenesis and impact treatment responses. Collaborating with proteins such as STAT1 and p53 IFIT3 can affect pathways involved in cell proliferation and apoptosis linking immune responses to cancer progression and therapy resistance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exhibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Up-regulates CDKN1A/p21 by down-regulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.
See full target information IFIT3
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Product promise

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