Mouse Polyclonal IFIT3/P60 antibody. Suitable for IP, ELISA, WB and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IFIT3.
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- Frontiers in immunology 13:9920602022An antigen processing and presentation signature for prognostic evaluation and immunotherapy selection in advanced gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe-Wei Wang,Mei-Dan Wang,Zi-Xi Li,Ben-Shun Hu,Jun-Jie Wu,Zheng-Dong Yuan,Xiao-Long Wu,Qin-Fang Yuan,Feng-Lai YuanPubMed 36311733
- eLife 11:2022Antiviral function and viral antagonism of the rapidly evolving dynein activating adaptor NINL.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDonté Alexander Stevens,Christopher Beierschmitt,Swetha Mahesula,Miles R Corley,John Salogiannis,Brian V Tsu,Bryant Cao,Andrew P Ryan,Hiroyuki Hakozawki,Samara L Reck-Peterson,Matthew D DaughertyPubMed 36222652
- Science advances 7:eabi67942021Deubiquitination of proteasome subunits by OTULIN regulates type I IFN production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPanfeng Tao,Shihao Wang,Seza Ozen,Pui Y Lee,Jiahui Zhang,Jun Wang,Huan Han,Zhaohui Yang,Ran Fang,Wanxia Li Tsai,Huanming Yang,Erdal Sag,Rezan Topaloglu,Ivona Aksentijevich,Xiaomin Yu,Qing ZhouPubMed 34797715
- Genome medicine 13:1682021Identification and drug-induced reversion of molecular signatures of Alzheimer's disease onset and progression in App, App, and 3xTg-AD mouse models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEduardo Pauls,Sergi Bayod,Lídia Mateo,Víctor Alcalde,Teresa Juan-Blanco,Marta Sánchez-Soto,Takaomi C Saido,Takashi Saito,Antoni Berrenguer-Llergo,Camille Stephan-Otto Attolini,Marina Gay,Eliandre de Oliveira,Miquel Duran-Frigola,Patrick AloyPubMed 34702310
- Journal of molecular biology 434:1672472021FTO Suppresses STAT3 Activation and Modulates Proinflammatory Interferon-Stimulated Gene Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael J McFadden,Matthew T Sacco,Kristen A Murphy,Moonhee Park,Nandan S Gokhale,Kim Y Somfleth,Stacy M HornerPubMed 34537236
- Journal of virology 94:2020Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Suppresses Type I and Type III Interferon Induction by Targeting RIG-I Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChi-You Chang et. al.PubMed 32295922
- Theranostics 10:7178-71922020Inflammatory IFIT3 renders chemotherapy resistance by regulating post-translational modification of VDAC2 in pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhefang Wang,Jie Qin,Jiangang Zhao,Jiahui Li,Dai Li,Marie Popp,Felix Popp,Hakan Alakus,Bo Kong,Qiongzhu Dong,Peter J Nelson,Yue Zhao,Christiane J BrunsPubMed 32641986
- mSphere 5:2020The mRNA Cap 2'--Methyltransferase CMTR1 Regulates the Expression of Certain Interferon-Stimulated Genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGraham D Williams et. al.PubMed 32404510
- Nucleic acids research 48:116-1292019ILF3 contributes to the establishment of the antiviral type I interferon program.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamir F Watson,Nicolas Bellora,Sara MaciasPubMed 31701124
- Hepatology (Baltimore, Md.) 66:152-1662017Hepatic IFIT3 predicts interferon-α therapeutic response in patients of hepatocellular carcinoma.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human IFIT3. Database link O14879
Reactivity data
Select an application| IP | ELISA | WB | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
Associated Products
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4 products for Alternative Product
Target data
Function
IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exhibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Up-regulates CDKN1A/p21 by down-regulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.
Alternative names
CIG-49, IFI60, IFIT4, ISG60, IFIT3, Interferon-induced protein with tetratricopeptide repeats 3, IFIT-3, CIG49, ISG-60, Interferon-induced 60 kDa protein, Interferon-induced protein with tetratricopeptide repeats 4, Retinoic acid-induced gene G protein, IFI-60K, IFIT-4, P60, RIG-G
Recommended products
Mouse Polyclonal IFIT3/P60 antibody. Suitable for IP, ELISA, WB and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IFIT3.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human IFIT3. Database link O14879
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IFIT3 also known as P60 is a member of the IFIT (Interferon-Induced Proteins with Tetratricopeptide Repeats) family of proteins. With a molecular mass of approximately 60 kDa IFIT3 is part of the antiviral response system. This protein is expressed in various tissues and is highly inducible by type I interferons. It functions to inhibit viral replication by interacting with viral RNA. The structure of IFIT3 includes tetratricopeptide repeat motifs which facilitate its role in protein-protein interactions.
- Biological function summary
IFIT3 plays a major role in the immune response against viral infections. The protein forms complexes with other IFIT family members like IFIT1 and IFIT2 to enhance antiviral efficacy. IFIT3 binds to foreign RNA lacking proper cap structures blocking viral protein synthesis. The protein's actions help strengthen the innate immune system and further control the spread of viral pathogens. IFIT3 also modulates cellular functions by engaging with signaling pathways that regulate inflammation and immune defense.
- Pathways
IFIT3 integrates into important signaling networks such as the interferon-mediated signal transduction pathway and the JAK-STAT signaling pathway. In these pathways IFIT3 along with related proteins like IRF3 and ISG15 helps amplify immune responses after detecting viral threats. Its involvement in these pathways ensures that antiviral states within cells are quickly established reducing viral survival and dissemination.
- Associated diseases and disorders
IFIT3 has significant implications for infection and cancer outcomes. In viral infections such as hepatitis C IFIT3 expression levels correlate with effective antiviral activity and prognosis. In cancer aberrant IFIT3 expression can influence tumorigenesis and impact treatment responses. Collaborating with proteins such as STAT1 and p53 IFIT3 can affect pathways involved in cell proliferation and apoptosis linking immune responses to cancer progression and therapy resistance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-IFIT3/P60 antibody (ab76818)
All lanes: Western blot - Anti-IFIT3/P60 antibody (ab76818) at 1/500 dilution
All lanes: Human colon lysate at 25 µg
SecondaryAll lanes: Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Western blot - Anti-IFIT3/P60 antibody (ab76818)
All lanes: Western blot - Anti-IFIT3/P60 antibody (ab76818) at 1/500 dilution
Lane 1: IFIT3 / P60 transfected 293T cell line at 25 µg
Lane 2: non transfected 293T cell line at 25 µg
SecondaryAll lanes: Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution
Predicted band size: 56 kDa
Observed band size: 65 kDa
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