AB76818

Anti-IFIT3/P60 antibody

Name: Anti-IFIT3/P60 antibody (ab76818) | Abcam
Brand: Abcam Limited
SKU: AB76818
Price: 535 USD
Availability: InStock

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(15 Publications)

Mouse Polyclonal IFIT3/P60 antibody. Suitable for IP, ELISA, WB and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IFIT3.

View Alternative Names

CIG-49, IFI60, IFIT4, ISG60, IFIT3, Interferon-induced protein with tetratricopeptide repeats 3, IFIT-3, CIG49, ISG-60, Interferon-induced 60 kDa protein, Interferon-induced protein with tetratricopeptide repeats 4, Retinoic acid-induced gene G protein, IFI-60K, IFIT-4, P60, RIG-G

2 Images

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Western blot - Anti-IFIT3/P60 antibody (AB76818)

All lanes:

Western blot - Anti-IFIT3/P60 antibody (ab76818) at 1/500 dilution

All lanes:

Human colon lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

Predicted band size: 56 kDa

Observed band size: 56 kDa

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Unknown

Western blot - Anti-IFIT3/P60 antibody (AB76818)

All lanes:

Western blot - Anti-IFIT3/P60 antibody (ab76818) at 1/500 dilution

Lane 1:

IFIT3 / P60 transfected 293T cell line at 25 µg

Lane 2:

non transfected 293T cell line at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

Predicted band size: 56 kDa

Observed band size: 65 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, ELISA, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IFIT3.

O14879

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IFIT3 also known as P60 is a member of the IFIT (Interferon-Induced Proteins with Tetratricopeptide Repeats) family of proteins. With a molecular mass of approximately 60 kDa IFIT3 is part of the antiviral response system. This protein is expressed in various tissues and is highly inducible by type I interferons. It functions to inhibit viral replication by interacting with viral RNA. The structure of IFIT3 includes tetratricopeptide repeat motifs which facilitate its role in protein-protein interactions.

Biological function summary

IFIT3 plays a major role in the immune response against viral infections. The protein forms complexes with other IFIT family members like IFIT1 and IFIT2 to enhance antiviral efficacy. IFIT3 binds to foreign RNA lacking proper cap structures blocking viral protein synthesis. The protein's actions help strengthen the innate immune system and further control the spread of viral pathogens. IFIT3 also modulates cellular functions by engaging with signaling pathways that regulate inflammation and immune defense.

Pathways

IFIT3 integrates into important signaling networks such as the interferon-mediated signal transduction pathway and the JAK-STAT signaling pathway. In these pathways IFIT3 along with related proteins like IRF3 and ISG15 helps amplify immune responses after detecting viral threats. Its involvement in these pathways ensures that antiviral states within cells are quickly established reducing viral survival and dissemination.

IFIT3 has significant implications for infection and cancer outcomes. In viral infections such as hepatitis C IFIT3 expression levels correlate with effective antiviral activity and prognosis. In cancer aberrant IFIT3 expression can influence tumorigenesis and impact treatment responses. Collaborating with proteins such as STAT1 and p53 IFIT3 can affect pathways involved in cell proliferation and apoptosis linking immune responses to cancer progression and therapy resistance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exhibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Up-regulates CDKN1A/p21 by down-regulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.

See full target information IFIT3

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 20:e1012287 PubMed38843304

2024

Temporal regulation of MDA5 inactivation by Caspase-3 dependent cleavage of 14-3-3η.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Jui Chan,Nien-Tzu Liu,Fu Hsin,Jia-Ying Lu,Jing-Yi Lin,Helene Minyi Liu

Frontiers in immunology 13:992060 PubMed36311733

2022

An antigen processing and presentation signature for prognostic evaluation and immunotherapy selection in advanced gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ke-Wei Wang,Mei-Dan Wang,Zi-Xi Li,Ben-Shun Hu,Jun-Jie Wu,Zheng-Dong Yuan,Xiao-Long Wu,Qin-Fang Yuan,Feng-Lai Yuan

eLife 11: PubMed36222652

2022

Antiviral function and viral antagonism of the rapidly evolving dynein activating adaptor NINL.

Applications

Unspecified application

Species

Unspecified reactive species

Donté Alexander Stevens,Christopher Beierschmitt,Swetha Mahesula,Miles R Corley,John Salogiannis,Brian V Tsu,Bryant Cao,Andrew P Ryan,Hiroyuki Hakozawki,Samara L Reck-Peterson,Matthew D Daugherty

Science advances 7:eabi6794 PubMed34797715

2021

Deubiquitination of proteasome subunits by OTULIN regulates type I IFN production.

Applications

Unspecified application

Species

Unspecified reactive species

Panfeng Tao,Shihao Wang,Seza Ozen,Pui Y Lee,Jiahui Zhang,Jun Wang,Huan Han,Zhaohui Yang,Ran Fang,Wanxia Li Tsai,Huanming Yang,Erdal Sag,Rezan Topaloglu,Ivona Aksentijevich,Xiaomin Yu,Qing Zhou

Genome medicine 13:168 PubMed34702310

2021

Identification and drug-induced reversion of molecular signatures of Alzheimer's disease onset and progression in App, App, and 3xTg-AD mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo Pauls,Sergi Bayod,Lídia Mateo,Víctor Alcalde,Teresa Juan-Blanco,Marta Sánchez-Soto,Takaomi C Saido,Takashi Saito,Antoni Berrenguer-Llergo,Camille Stephan-Otto Attolini,Marina Gay,Eliandre de Oliveira,Miquel Duran-Frigola,Patrick Aloy

Journal of molecular biology 434:167247 PubMed34537236

2021

FTO Suppresses STAT3 Activation and Modulates Proinflammatory Interferon-Stimulated Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J McFadden,Matthew T Sacco,Kristen A Murphy,Moonhee Park,Nandan S Gokhale,Kim Y Somfleth,Stacy M Horner

Journal of virology 94: PubMed32295922

2020

Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Suppresses Type I and Type III Interferon Induction by Targeting RIG-I Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chi-You Chang,Helene Minyi Liu,Ming-Fu Chang,Shin C Chang

Theranostics 10:7178-7192 PubMed32641986

2020

Inflammatory IFIT3 renders chemotherapy resistance by regulating post-translational modification of VDAC2 in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhefang Wang,Jie Qin,Jiangang Zhao,Jiahui Li,Dai Li,Marie Popp,Felix Popp,Hakan Alakus,Bo Kong,Qiongzhu Dong,Peter J Nelson,Yue Zhao,Christiane J Bruns

mSphere 5: PubMed32404510

2020

The mRNA Cap 2'--Methyltransferase CMTR1 Regulates the Expression of Certain Interferon-Stimulated Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Graham D Williams,Nandan S Gokhale,Daltry L Snider,Stacy M Horner

Nucleic acids research 48:116-129 PubMed31701124

2019

ILF3 contributes to the establishment of the antiviral type I interferon program.

Applications

Unspecified application

Species

Unspecified reactive species

Samir F Watson,Nicolas Bellora,Sara Macias

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-IFIT3/P60 antibody

Western blot - Anti-IFIT3/P60 antibody (AB76818)

Western blot - Anti-IFIT3/P60 antibody (AB76818)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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