AB118045

Anti-IFIT3/P60 antibody [OTI1G1]

Name: Anti-IFIT3/P60 antibody [OTI1G1] (ab118045) | Abcam
Brand: Abcam Limited
SKU: AB118045
Price: 495 USD
Availability: InStock

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Mouse Monoclonal IFIT3/P60 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with African green monkey, Human, Transfected cell lysate samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IFIT3.

View Alternative Names

CIG-49, IFI60, IFIT4, ISG60, IFIT3, Interferon-induced protein with tetratricopeptide repeats 3, IFIT-3, CIG49, ISG-60, Interferon-induced 60 kDa protein, Interferon-induced protein with tetratricopeptide repeats 4, Retinoic acid-induced gene G protein, IFI-60K, IFIT-4, P60, RIG-G

5 Images

Supplier Data

Western blot - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

All lanes:

Western blot - Anti-IFIT3/P60 antibody [OTI1G1] (ab118045) at 1/200 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) extracts at 35 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts at 35 µg

Lane 3:

SVT2 extracts at 35 µg

Lane 4:

A549 (Human lung carcinoma cell line) extracts at 35 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) extracts at 35 µg

Lane 6:

Jurkat (Human T cell leukemia cell line from peripheral blood) extracts at 35 µg

Lane 7:

MDCK (Canine kidney cell line) extracts at 35 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma cell line) extracts at 35 µg

Lane 9:

MCF7 (Human breast adenocarcinoma cell line) extracts at 35 µg

Predicted band size: 56 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

pCMV6-ENTRY IFIT3/P60 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for IFIT3/P60 using ab118045 at a 1/100 dilution in ICC/IF.

Flow Cytometry (Intracellular) - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

ab118045 at 1/100 dilution staining IFIT3/P60 in Jurkat (Human T cell leukemia cell line from peripheral blood) cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

HT-29 (Human colorectal adenocarcinoma cell line) cells stained for IFIT3/P60 using ab118045 at a 1/100 dilution in ICC/IF.

Unknown

Western blot - Anti-IFIT3/P60 antibody [OTI1G1] (AB118045)

All lanes:

Western blot - Anti-IFIT3/P60 antibody [OTI1G1] (ab118045) at 1/2000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells, transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cells, transfected with pCMV6-ENTRY IFIT3/P60 cDNA at 5 µg

Predicted band size: 56 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1G1

Isotype

IgG1

Carrier free

Reacts with

Human, African green monkey

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IFIT3.

O14879

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant by affinity chromatography.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IFIT3 also known as P60 is a member of the IFIT (Interferon-Induced Proteins with Tetratricopeptide Repeats) family of proteins. With a molecular mass of approximately 60 kDa IFIT3 is part of the antiviral response system. This protein is expressed in various tissues and is highly inducible by type I interferons. It functions to inhibit viral replication by interacting with viral RNA. The structure of IFIT3 includes tetratricopeptide repeat motifs which facilitate its role in protein-protein interactions.

Biological function summary

IFIT3 plays a major role in the immune response against viral infections. The protein forms complexes with other IFIT family members like IFIT1 and IFIT2 to enhance antiviral efficacy. IFIT3 binds to foreign RNA lacking proper cap structures blocking viral protein synthesis. The protein's actions help strengthen the innate immune system and further control the spread of viral pathogens. IFIT3 also modulates cellular functions by engaging with signaling pathways that regulate inflammation and immune defense.

Pathways

IFIT3 integrates into important signaling networks such as the interferon-mediated signal transduction pathway and the JAK-STAT signaling pathway. In these pathways IFIT3 along with related proteins like IRF3 and ISG15 helps amplify immune responses after detecting viral threats. Its involvement in these pathways ensures that antiviral states within cells are quickly established reducing viral survival and dissemination.

IFIT3 has significant implications for infection and cancer outcomes. In viral infections such as hepatitis C IFIT3 expression levels correlate with effective antiviral activity and prognosis. In cancer aberrant IFIT3 expression can influence tumorigenesis and impact treatment responses. Collaborating with proteins such as STAT1 and p53 IFIT3 can affect pathways involved in cell proliferation and apoptosis linking immune responses to cancer progression and therapy resistance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exhibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Up-regulates CDKN1A/p21 by down-regulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.

See full target information IFIT3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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