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AB236735

Anti-IFITM2 antibody

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(1 Publication)

Rabbit Polyclonal IFITM2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IFITM2 aa 1-100.

View Alternative Names

Interferon-induced transmembrane protein 2, Dispanin subfamily A member 2c, Interferon-inducible protein 1-8D, DSPA2c, IFITM2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-IFITM2 antibody (AB236735)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IFITM2 antibody (AB236735)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for IFITM2 (green) using ab236735 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFITM2 antibody (AB236735)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFITM2 antibody (AB236735)

Paraffin-embedded human lung cancer tissue stained for IFITM2 using ab236735 at 1/700 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

Western blot - Anti-IFITM2 antibody (AB236735)
  • WB

Supplier Data

Western blot - Anti-IFITM2 antibody (AB236735)

All lanes:

Western blot - Anti-IFITM2 antibody (ab236735) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 4:

A549 (human lung carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human IFITM2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q01629

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IFITM2 protein also known as Interferon-Induced Transmembrane Protein 2 is a member of the IFITM family which includes IFITM1 and IFITM3. IFITM2 has a molecular mass of approximately 14 kDa. It is found mainly in the plasma membrane and is expressed in various tissues notably immune cells. It functions as part of the cellular response to viral infections acting mechanically to restrict virus entry into the host cell and prevent replication of various viruses.
Biological function summary

The role of IFITM2 extends beyond mechanical blocking as it participates in the immune response by inhibiting the fusion of viral membranes with endosomal membranes. IFITM2 operates together with IFITM1 and IFITM3 forming a complex that boosts its antiviral capacity. The protein contributes to the defense mechanism against a broad range of viral pathogens including influenza and dengue viruses.

Pathways

Research shows that IFITM2 is involved in the interferon signaling pathway which regulates immune response to pathogens. In this pathway IFITM proteins are part of the type I and type II interferon responses enhancing cellular resistance to viral infection. The protein collaborates with others such as STAT1 in maintaining cellular defenses against pathogens.

Several studies have connected IFITM2 to influenza and other viral infections. Altered function or expression of IFITM2 could relate to increased susceptibility to some viral infections or influence the disease progression. The interacts with proteins such as IFITM3 further implicating its involvement in the clinical outcomes of these viral diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol (PubMed : 26354436, PubMed : 33563656). Active against multiple viruses, including influenza A virus, SARS coronaviruses (SARS-CoV and SARS-CoV-2), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1), hepatitis C virus (HCV) and vesicular stomatitis virus (VSV) (PubMed : 26354436, PubMed : 33239446, PubMed : 33270927, PubMed : 33563656). Can inhibit : influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV and SARS-CoV-2 S protein-mediated viral entry and VSV G protein-mediated viral entry (PubMed : 33563656). Induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. In hepatocytes, IFITM proteins act in a coordinated manner to restrict HCV infection by targeting the endocytosed HCV virion for lysosomal degradation (PubMed : 26354436). IFITM2 and IFITM3 display anti-HCV activity that may complement the anti-HCV activity of IFITM1 by inhibiting the late stages of HCV entry, possibly in a coordinated manner by trapping the virion in the endosomal pathway and targeting it for degradation at the lysosome (PubMed : 26354436).
See full target information IFITM2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36499015

2022

Fusion Cell Markers in Circulating Tumor Cells from Patients with High-Grade Ovarian Serous Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Paula Carreta Ruano,Andrea Paiva Gadelha Guimarães,Alexcia C Braun,Bianca C T C P Flores,Milena Shizue Tariki,Emne A Abdallah,Jacqueline Aparecida Torres,Diana Noronha Nunes,Bruna Tirapelli,Vladmir C Cordeiro de Lima,Marcello Ferretti Fanelli,Pierre-Emmanuel Colombo,Alexandre André Balieiro Anastácio da Costa,Catherine Alix-Panabières,Ludmilla Thomé Domingos Chinen
View all publications
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