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Rabbit Recombinant Monoclonal IFITM2 antibody. Suitable for Flow Cyt (Intra), ICC/IF, WB and reacts with Transfected cell line - Human, Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (AB323747), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (AB323747), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (AB323747), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (AB323747), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (AB323747), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)ICC/IFWB
Human
Tested
Tested
Tested
Transfected cell line - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/5000
Notes

-

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human
Dilution info
-
Notes

-

Target data

Function

IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol (PubMed:26354436, PubMed:33563656). Active against multiple viruses, including influenza A virus, SARS coronaviruses (SARS-CoV and SARS-CoV-2), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1), hepatitis C virus (HCV) and vesicular stomatitis virus (VSV) (PubMed:26354436, PubMed:33239446, PubMed:33270927, PubMed:33563656). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV and SARS-CoV-2 S protein-mediated viral entry and VSV G protein-mediated viral entry (PubMed:33563656). Induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. In hepatocytes, IFITM proteins act in a coordinated manner to restrict HCV infection by targeting the endocytosed HCV virion for lysosomal degradation (PubMed:26354436). IFITM2 and IFITM3 display anti-HCV activity that may complement the anti-HCV activity of IFITM1 by inhibiting the late stages of HCV entry, possibly in a coordinated manner by trapping the virion in the endosomal pathway and targeting it for degradation at the lysosome (PubMed:26354436).

Additional Targets

IFITM3

Alternative names

Interferon-induced transmembrane protein 2, Dispanin subfamily A member 2c, Interferon-inducible protein 1-8D, DSPA2c, IFITM2

Rabbit Recombinant Monoclonal IFITM2 antibody. Suitable for Flow Cyt (Intra), ICC/IF, WB and reacts with Transfected cell line - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28942-88
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Flow Cytometry (Intracellular) - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (ab323747), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (ab323747)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A549 (human lung carcinoma epithelial cell) treated with 10ng/mL IFN-alpha 1 for 16 hours (Green) / Untreated A549 (Magenta) cells labelling IFITM2+IFITM3 with ab323747 at 1/50 dilution (1 ug) / Magenta and Green compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (ab323747), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (ab323747)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (upper left) / 293T (human embryonic kidney epithelial cell) cells transfected with an empty expression vector containing a Myc tag (upper middle) / 293T cells transfected with a IFITM1 expression vector containing a GFP tag (down left) / 293T cells transfected with a IFITM2 expression vector containing a GFP tag (down middle) / 293T cells transfected with a IFITM3 expression vector containing a GFP tag(down right) cells labelling IFITM2+IFITM3 with ab323747 at 1/5000 dilution (0.01 ug) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (ab323747), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (ab323747)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) treated with 10ng/mL IFN-alpha 1 for 16 hours (Green) / Untreated HeLa (Magenta) cells labelling IFITM2+IFITM3 with ab323747 at 1/50 dilution (1 ug) / Magenta and Green compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (ab323747), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (ab323747)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling IFITM2+IFITM3 with ab323747 at 1/250 (2.004 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ 1000 2ug/ml dilution (Green).

    Confocal image showing membranous and cytoplasmic staining in A549 cell line treated with IFN alpha (1000 U/ml) for 24h, and weak staining in A549 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/ 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (ab323747), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (ab323747)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling IFITM2+IFITM3 with ab323747 at 1/250 (2.004 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ 1000 2ug/ml dilution (Green).

    Confocal image showing membranous and cytoplasmic staining in HeLa cell line treated with IFN alpha 1 (10ng/ml) for 16h, and weak cytoplasmic staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/ 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution.

  • Western blot - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (ab323747), expandable thumbnail

    Western blot - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (ab323747)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Daudi

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 34078739; PMID: 34321474; PMID: 30643282).

    Lane 3 is incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 1-2 are incubated with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (ab323747) at 1/1000 dilution

    Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 2: Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 20 µg

    Lane 3: Human liver tissue lysate at 20 µg

    Secondary

    Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Lane 3: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 15 kDa, 36 kDa

    Exposure time: 70s

  • Western blot - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (ab323747), expandable thumbnail

    Western blot - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (ab323747)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 34078739; PMID: 34321474; PMID: 30643282).

    The expression of IFITM2 and IFITM3 is up-regulated in response to IFN alpha 1 or IFN beta treatment (PMID: 34078739; PMID: 34321474; PMID: 30643282).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-4: 15 seconds; Lanes 5-6: 5 seconds

    All lanes: Western blot - Anti-IFITM2+IFITM3 antibody [EPR28942-88] (ab323747) at 1/1000 dilution

    Lanes 1 and 3: Untreated A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: A549 treated with 1000U/ml IFN alpha 1 for 24 hours whole cell lysate at 20 µg

    Lane 4: A549 treated with 1000U/ml IFN beta 1 for 24 hours whole cell lysate at 20 µg

    Lane 5: Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: HeLa treated with 10ng/ml IFN alpha 1 for 16 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 15 kDa, 36 kDa

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Product protocols

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