AB224197

Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171]

RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal IFN gamma Receptor beta/AF-1 antibody. Suitable for Flow Cyt, ELISA, ICC/IF and reacts with Human, Recombinant full length protein - Human samples. Cited in 7 publications.

View Alternative Names

IFNGT1, IFNGR2, Interferon gamma receptor 2, IFN-gamma receptor 2, IFN-gamma-R2, Interferon gamma receptor accessory factor 1, Interferon gamma receptor beta-chain, Interferon gamma transducer 1, AF-1, IFN-gamma-R-beta

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (AB224197)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (AB224197)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing positive staining in HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct. The nuclear counterstain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red) and Myc-Tag (9B11) Mouse mAb (Alexa Fluor^® 647 Conjugate) (white).
The negative control is the secondary antibody only.

Flow Cytometry - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (AB224197)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (AB224197)

Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct cell line labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/400 dilution.

Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Cells were surface stained with isotype control Rabbit monoclonal IgG (ab172730) (Left panel) or IFN gamma Receptor beta/AF-1 (Right panel), then co-stained with Alexa Fluor^® 647 conjugated-Myc-tag antibody.

Gated on viable cells.

ELISA - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (AB224197)

ELISA

Lab

ELISA - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (AB224197)

ELISA analysis of Human IFNGR2 recombinant protein at 1000 ng/mL with ab224197. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Carrier free

Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22171

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt, ICC/IF, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/400", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/400", "FlowCyt-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IFN gamma Receptor beta also known as IFN-γ receptor β chain or IFN gamma receptor AF-1 plays a significant role in the immune response. This receptor is part of the interferon gamma receptor complex which binds the cytokine IFN-gamma. The beta chain contributes to the formation of the receptor complex that allows functional IFN-gamma signaling. Typically the receptor is expressed on the surface of immune cells like lymphocytes and macrophages facilitating communication and response to infections and immune challenges. The exact molecular mass can vary depending on glycosylation often giving an approximate mass of 35-50 kDa.

Biological function summary

IFN gamma Receptor beta is essential for the activation of the immune system. It partners with IFN gamma Receptor alpha in the receptor complex to mediate important biological effects such as activation of macrophages and enhancement of antigen presentation. This activity boosts the cellular immune response allowing cells to better combat microbial infections and tumor growth. Without functional receptor activity cells would not effectively respond to IFN-gamma signals impeding proper immune function.

Pathways

IFN gamma Receptor beta is involved in the JAK-STAT signaling pathway which is important for mediating IFN-gamma signaling. Once IFN-gamma binds to its receptor including the beta chain it triggers the activation of JAK kinases which in turn phosphorylates STAT1. Phosphorylated STAT1 dimerizes translocates to the nucleus and influences the expression of immune regulatory genes. This pathway connects the receptor with key immune regulatory proteins playing an important role in the immune response.

IFN gamma Receptor beta is associated with conditions such as chronic granulomatous disease and various mycobacterial infections. These conditions often result from immune system malfunctions where the receptor cannot properly signal IFN-gamma activity leading to an impaired defense against pathogens. The involvement of the receptor in immune pathways links it to other critical proteins like IFN gamma Receptor alpha and STAT1 both of which might be implicated in IFN-gamma-related dysfunctions contributing to these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Associates with IFNGR1 to form a receptor for the cytokine interferon gamma (IFNG) (PubMed : 7615558, PubMed : 7673114, PubMed : 8124716). Ligand binding stimulates activation of the JAK/STAT signaling pathway (PubMed : 15356148, PubMed : 7673114, PubMed : 8124716). Required for signal transduction in contrast to other receptor subunit responsible for ligand binding (PubMed : 7673114).

See full target information IFNGR2

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

MedComm 6:e70311 PubMed40761479

2025

Nanoparticle-Delivered siRNA Targeting NSUN4 Relieves Systemic Lupus Erythematosus through Declining Mitophagy-Mediated CD8+T Cell Exhaustion.

Applications

Unspecified application

Species

Unspecified reactive species

Bincheng Ren,Kaini He,Ning Wei,Shanshan Liu,Xiaoguang Cui,Xin Yang,Xiaojing Cheng,Tian Tian,Ru Gu,Xueyi Li

Journal of nanobiotechnology 23:519 PubMed40671009

2025

CXCL12-targeting siRNA nanoparticles alleviate immunosuppression and inhibit tumor progression in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyao Zhang,Hong Jiang,Chengkuan Zhao,Yanli Lei,Shaojie Liu,Chengcheng Xu,Xiaoshan Chen,Danling Zheng,Xiaolong Wu,Xinyue Lin,Wang Chen,Yun Chen,Jianxiang Huang,XiaoLong Wei,Yihui Huang,Chaoxian Lin

ACS omega 10:17416-17434 PubMed40352528

2025

Mercury Can Bond to α-Carbon of Curcumin Increasing Stability in Aqueous Medium and Demonstrated Selective Cytotoxicity against Acute Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Sougata Mondal,Upasana Das,Oyendrila Ghosh,Bidisha Maiti,Shuvam Halder,Uttam Pal,Kalyan Kusum Mukherjee,Supratim Ghosh

BMC immunology 26:30 PubMed40200137

2025

Fecal microbiota transplantation enhanced the effect of chemoimmunotherapy by restoring intestinal microbiota in LLC tumor-bearing mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xinmeng Wang,Qian Geng,Hua Jiang,Jingyan Yue,Chunjian Qi,Lanqun Qin

Research (Washington, D.C.) 7:0493 PubMed39381792

2024

Melatonin Induces Analgesic Effects through MT Receptor-Mediated Neuroimmune Modulation in the Mice Anterior Cingulate Cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wang,Junxiang Gu,Fujuan Ma,Yi Wei,Pan Wang,Shanming Yang,Xianxia Yan,Yifan Xiao,Keke Xing,Anxin Lou,Liru Zheng,Tingting Cao,Dayu Zhu,Jinlian Li,Luoying Zhang,Yunqing Li,Tao Chen

World journal of gastrointestinal oncology 16:3118-3157 PubMed39072171

2024

Kombo knife combined with sorafenib in liver cancer treatment: Efficacy and safety under immune function influence.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Cao,Pei-Pei Li,Bing-Li Qiao,Quan-Wang Li

Communications biology 5:945 PubMed36088469

2022

Low-dose radiation exaggerates HFD-induced metabolic dysfunction by gut microbiota through PA-PYCR1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Ju,Peiyu Guo,Jing Xiang,Ridan Lei,Guofeng Ren,Meiling Zhou,Xiandan Yang,Pingkun Zhou,Ruixue Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171]

Immunocytochemistry/ Immunofluorescence - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (AB224197)

Flow Cytometry - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (AB224197)

ELISA - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (AB224197)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise