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AB240559

Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free

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Rabbit Recombinant Monoclonal IFN gamma Receptor beta/AF-1 antibody. Carrier free. Suitable for Flow Cyt, ELISA, ICC/IF and reacts with Human, Recombinant fragment - Human samples.

View Alternative Names

IFNGT1, IFNGR2, Interferon gamma receptor 2, IFN-gamma receptor 2, IFN-gamma-R2, Interferon gamma receptor accessory factor 1, Interferon gamma receptor beta-chain, Interferon gamma transducer 1, AF-1, IFN-gamma-R-beta

3 Images
Flow Cytometry - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (AB240559)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (AB240559)

Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct cell line labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/400 dilution.

Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Cells were surface stained with isotype control Rabbit monoclonal IgG (ab172730) (Left panel) or IFN gamma receptor beta/AF-1 (Right panel), then co-stained with Alexa Fluor® 647 conjugated-Myc-tag antibody.

Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224197).

Immunocytochemistry/ Immunofluorescence - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (AB240559)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (AB240559)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing positive staining in HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct. The nuclear counterstain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red) and Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) (white).
The negative control is the secondary antibody only.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab224197).

ELISA - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (AB240559)
  • ELISA

Lab

ELISA - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (AB240559)

This data was developed using ab224197, the same antibody clone in a different buffer formulation.ELISA analysis of Human IFNGR2 recombinant protein at 1000 ng/mL with ab224197. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

  • Unconjugated

    Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22171

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Flow Cyt, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab240559 is the carrier-free version of ab224197.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IFN gamma Receptor beta also known as IFN-γ receptor β chain or IFN gamma receptor AF-1 plays a significant role in the immune response. This receptor is part of the interferon gamma receptor complex which binds the cytokine IFN-gamma. The beta chain contributes to the formation of the receptor complex that allows functional IFN-gamma signaling. Typically the receptor is expressed on the surface of immune cells like lymphocytes and macrophages facilitating communication and response to infections and immune challenges. The exact molecular mass can vary depending on glycosylation often giving an approximate mass of 35-50 kDa.
Biological function summary

IFN gamma Receptor beta is essential for the activation of the immune system. It partners with IFN gamma Receptor alpha in the receptor complex to mediate important biological effects such as activation of macrophages and enhancement of antigen presentation. This activity boosts the cellular immune response allowing cells to better combat microbial infections and tumor growth. Without functional receptor activity cells would not effectively respond to IFN-gamma signals impeding proper immune function.

Pathways

IFN gamma Receptor beta is involved in the JAK-STAT signaling pathway which is important for mediating IFN-gamma signaling. Once IFN-gamma binds to its receptor including the beta chain it triggers the activation of JAK kinases which in turn phosphorylates STAT1. Phosphorylated STAT1 dimerizes translocates to the nucleus and influences the expression of immune regulatory genes. This pathway connects the receptor with key immune regulatory proteins playing an important role in the immune response.

IFN gamma Receptor beta is associated with conditions such as chronic granulomatous disease and various mycobacterial infections. These conditions often result from immune system malfunctions where the receptor cannot properly signal IFN-gamma activity leading to an impaired defense against pathogens. The involvement of the receptor in immune pathways links it to other critical proteins like IFN gamma Receptor alpha and STAT1 both of which might be implicated in IFN-gamma-related dysfunctions contributing to these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Associates with IFNGR1 to form a receptor for the cytokine interferon gamma (IFNG) (PubMed : 7615558, PubMed : 7673114, PubMed : 8124716). Ligand binding stimulates activation of the JAK/STAT signaling pathway (PubMed : 15356148, PubMed : 7673114, PubMed : 8124716). Required for signal transduction in contrast to other receptor subunit responsible for ligand binding (PubMed : 7673114).
See full target information IFNGR2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
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