Anti-IFNAR2 antibody [EPR24706-26] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IFNAR2 antibody [EPR24706-26] - BSA and Azide free (AB308499)

This data was developed using ab308498, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U266B1 (human multiple myeloma B lymphocyte) cells labelling IFNAR2 with ab308498 at 1/50 (9.46 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous staining in U266B1 cell line.Negative control : T-47D. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-IFNAR2 antibody [EPR24706-26] - BSA and Azide free (AB308499)

This data was developed using ab308498, the same antibody clone in a different buffer formulation. Flow cytometric analysis of T-47D (human ductal breast epithelial tumor epithelial cell, Left) / U266b1 (human multiple myeloma B lymphocyte, Right) cells labelling IFNAR2 with ab308498 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : T-47D. Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-IFNAR2 antibody [EPR24706-26] - BSA and Azide free (AB308499)

This data was developed using ab308498, the same antibody clone in a different buffer formulation. Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / HEL (human erythroleukemia erythroblast, Right) cells labelling IFNAR2 with ab308498 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : MCF7. Gated on viable cells.

• IP

Supplier Data

Immunoprecipitation - Anti-IFNAR2 antibody [EPR24706-26] - BSA and Azide free (AB308499)

This data was developed using ab308498, the same antibody clone in a different buffer formulation.

IFNAR2 was immunoprecipitated from 0.35 mg U266B1 (human multiple myeloma B lymphocyte) whole cell lysate with ab308498 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308498 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : U266B1 (human multiple myeloma B lymphocyte) whole cell lysate
Lane 2 : ab308498 IP in U266B1 (human multiple myeloma B lymphocyte) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab308498 in U266B1 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds

All lanes:

Immunoprecipitation - Anti-IFNAR2 antibody [EPR24706-26] (<a href='/en-us/products/primary-antibodies/ifnar2-antibody-epr24706-26-ab308498'>ab308498</a>) at 1/1000 dilution

All lanes:

U266B1 (human multiple myeloma B lymphocyte) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 57 kDa,80 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-IFNAR2 antibody [EPR24706-26] - BSA and Azide free (AB308499)

This data was developed using ab308498, the same antibody clone in a different buffer formulation.

Low expression : T-47D.

Samples are non-boiled as boiling may cause protein aggregation.

All lanes:

Western blot - Anti-IFNAR2 antibody [EPR24706-26] (<a href='/en-us/products/primary-antibodies/ifnar2-antibody-epr24706-26-ab308498'>ab308498</a>) at 1/1000 dilution

Lane 1:

U266B1 (human multiple myeloma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

HEL (erythroleukemia erythroblast) whole cell lysate at 20 µg

Lane 3:

T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 57-80 kDa

false

Exposure time: 80s