AB154400

Anti-IFNGR1 antibody

Name: Anti-IFNGR1 antibody (ab154400) | Abcam
Brand: Abcam Limited
SKU: AB154400
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal IFNGR1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IFNGR1 aa 200-450.

View Alternative Names

CD119, Interferon gamma receptor 1, IFN-gamma receptor 1, IFN-gamma-R1, CDw119, Interferon gamma receptor alpha-chain, IFN-gamma-R-alpha, IFNGR1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFNGR1 antibody (AB154400)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFNGR1 antibody (AB154400)

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling IFNGR1 with ab154400 antibody at 1/250.

Supplier Data

Western blot - Anti-IFNGR1 antibody (AB154400)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-IFNGR1 antibody (ab154400) at 1/500 dilution

Lane 1:

Wild-type HeLa whole cell lysate at 30 µg

Lane 2:

IFNGR1 knockout HeLa whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 54 kDa

false

Unknown

Western blot - Anti-IFNGR1 antibody (AB154400)

10% SDS PAGE

All lanes:

Western blot - Anti-IFNGR1 antibody (ab154400) at 1/3000 dilution

Lane 1:

THP-1 whole cell lysate at 30 µg

Lane 2:

K562 whole cell lysate at 30 µg

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human IFNGR1 aa 200-450. The exact immunogen used to generate this antibody is proprietary information.

P15260

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IFNGR1 protein also known as Interferon Gamma Receptor 1 is an integral component of the cell surface receptor for interferon-gamma (IFN-γ). It possesses a mass of approximately 53 kDa. IFNGR1 is expressed in various cell types including immune cells such as T cells and macrophages and is also present in other cell lines like HEK 293. This receptor binds to its specific ligand IFN-γ initiating a series of intracellular events that are essential for immune response modulation.

Biological function summary

IFNGR1 functions as part of the interferon-gamma receptor complex working alongside the IFNGR2 subunit. This interaction with IFNGR2 is important for the receptor to properly transmit signals inside the cell. The binding of interferon-gamma to this receptor complex activates the JAK-STAT signaling pathway promoting the transcription of genes that enhance the antimicrobial activity of immune cells and regulate cellular immunity.

Pathways

The IFNGR1 protein plays an important role in the JAK-STAT signaling pathway which is critical for mediating responses to interferon-gamma. Upon activation by IFN-γ IFNGR1 recruits JAK kinases leading to the phosphorylation of STAT1 a significant transcription factor. STAT1 then dimerizes and translocates to the nucleus where it induces the expression of genes involved in immune defense and inflammation. This process is vital for the body's ability to handle infections and other immunological challenges.

IFNGR1 is associated with susceptibility to infections and certain immune-related disorders. Mutations or deficiencies in IFNGR1 can lead to Mendelian Susceptibility to Mycobacterial Disease (MSMD) where individuals show increased vulnerability to mycobacterial infections. Additionally improper signaling through IFNGR1 is linked to chronic granulomatous disease which can involve defective functioning of the NADPH oxidase complex. Understanding IFNGR1's function and interactions is important for exploring new therapeutic strategies for these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 20015550). Associates with transmembrane accessory factor IFNGR2 to form a functional receptor (PubMed : 10986460, PubMed : 2971451, PubMed : 7615558, PubMed : 7617032, PubMed : 7673114). Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to dimerization, translocation to the nucleus, and stimulation of target gene transcription (PubMed : 28883123). STAT3 can also be activated in a similar manner although activation seems weaker. IFNGR1 intracellular domain phosphorylation also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1 (By similarity).

See full target information IFNGR1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 4:101006 PubMed37044092

2023

MYC activation impairs cell-intrinsic IFNγ signaling and confers resistance to anti-PD1/PD-L1 therapy in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Juan J Alburquerque-Bejar,Pablo Navajas-Chocarro,Maria Saigi,Ana Ferrero-Andres,Juan M Morillas,Andrea Vilarrubi,Antonio Gomez,José L Mate,Ana M Munoz-Marmol,Octavio A Romero,Pedro Blecua,Veronica Davalos,Manel Esteller,Eva Pros,Paula Llabata,Manuel Torres-Diz,Anna Esteve-Codina,Montse Sanchez-Cespedes

iScience 25:104193 PubMed35479410

2022

EMT alterations in the solute carrier landscape uncover SLC22A10/A15 imposed vulnerabilities in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Debasis Nayak,Brenna Weadick,Avinash K Persaud,Radhika Raj,Reena Shakya,Junan Li,Moray J Campbell,Rajgopal Govindarajan

Cancer science 112:5100-5113 PubMed34626022

2021

Aberrant expression of MYD88 via RNA-controlling CNOT4 and EXOSC3 in colonic mucosa impacts generation of colonic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Masumi Tsuda,Misa Noguchi,Tsuyoshi Kurai,Yuji Ichihashi,Koki Ise,Lei Wang,Yusuke Ishida,Mishie Tanino,Satoshi Hirano,Masahiro Asaka,Shinya Tanaka

Viruses 13: PubMed34452514

2021

The Ebola Virus Interferon Antagonist VP24 Undergoes Active Nucleocytoplasmic Trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Angela R Harrison,Cassandra T David,Stephen M Rawlinson,Gregory W Moseley

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IFNGR1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFNGR1 antibody (AB154400)

Western blot - Anti-IFNGR1 antibody (AB154400)

Western blot - Anti-IFNGR1 antibody (AB154400)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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