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Rabbit Recombinant Monoclonal IFNGR1 antibody. Suitable for ICC/IF, IP, Flow Cyt, WB and reacts with Mouse samples. Cited in 1 publication.

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Images

Flow Cytometry - Anti-IFNGR1 antibody [EPR24127-89] (AB280353), expandable thumbnail
  • Western blot - Anti-IFNGR1 antibody [EPR24127-89] (AB280353), expandable thumbnail
  • Flow Cytometry - Anti-IFNGR1 antibody [EPR24127-89] (AB280353), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IFNGR1 antibody [EPR24127-89] (AB280353), expandable thumbnail
  • Immunoprecipitation - Anti-IFNGR1 antibody [EPR24127-89] (AB280353), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Target data

Function

Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (, PubMed:20926559, PubMed:27286456). Associates with transmembrane accessory factor IFNGR2 to form a functional receptor (PubMed:2530582, PubMed:2532365, PubMed:2137461, PubMed:2531896, PubMed:2530216). Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to its dimerization, translocation to the nucleus, and stimulation of target gene transcription (PubMed:19889125). STAT3 can also be activated in a similar manner although activation seems weaker (PubMed:15284232). IFNGR1 intracellular domain phosphorylation also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1 (PubMed:15522878).

Alternative names

Interferon gamma receptor 1, IFN-gamma receptor 1, IFN-gamma-R1, Interferon gamma receptor alpha-chain, IFN-gamma-R-alpha, Ifngr1, Ifngr

Recommended products

Rabbit Recombinant Monoclonal IFNGR1 antibody. Suitable for ICC/IF, IP, Flow Cyt, WB and reacts with Mouse samples. Cited in 1 publication.

Alternative names

Interferon gamma receptor 1, IFN-gamma receptor 1, IFN-gamma-R1, Interferon gamma receptor alpha-chain, IFN-gamma-R-alpha, Ifngr1, Ifngr

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR24127-89

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry - Anti-IFNGR1 antibody [EPR24127-89] (ab280353), expandable thumbnail

    Flow Cytometry - Anti-IFNGR1 antibody [EPR24127-89] (ab280353)

    Flow cytometric analysis of 4T1 (mouse mammary gland carcinoma epithelial cell, left)/ EL4 (mouse lymphoma T lymphocyte, right) cells labelling IFNGR1 with ab280353 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Low expression: 4T1(PMID:12154047) Gated on viable cells.

  • Western blot - Anti-IFNGR1 antibody [EPR24127-89] (ab280353), expandable thumbnail

    Western blot - Anti-IFNGR1 antibody [EPR24127-89] (ab280353)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Low expression: 4T1(PMID:12154047)

    Exposure time: 74 seconds

    All lanes: Western blot - Anti-IFNGR1 antibody [EPR24127-89] (ab280353) at 1/1000 dilution

    Lane 1: EL4 (mouse lymphoma T lymphocyte), whole cell lysate at 20 µg

    Lane 2: 4T1 (mouse mammary gland carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 54 kDa

    Observed band size: 70-95 kDa

    This data was developed using ab280353, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Low expression: 4T1(PMID:12154047)

    Exposure time: 74 seconds

  • Flow Cytometry - Anti-IFNGR1 antibody [EPR24127-89] (ab280353), expandable thumbnail

    Flow Cytometry - Anti-IFNGR1 antibody [EPR24127-89] (ab280353)

    Flow cytometric analysis of Mouse blood cells cells labelling IFNGR1 with ab280353 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-IFNGR1 antibody [EPR24127-89] (ab280353), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IFNGR1 antibody [EPR24127-89] (ab280353)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 cells labelling IFNGR1 with ab280353 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in EL4 cell line. Low expression control:4T1 (PMID: 12154047) is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunoprecipitation - Anti-IFNGR1 antibody [EPR24127-89] (ab280353), expandable thumbnail

    Immunoprecipitation - Anti-IFNGR1 antibody [EPR24127-89] (ab280353)

    IFNGR1 was immunoprecipitated from 0.35 mg EL4 (mouse lymphoma T lymphocyte), whole cell lysate 10 ug with ab280353 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280353 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: EL4 (mouse lymphoma T lymphocyte), whole cell lysate 10 ug

    Lane 2: ab280353 IP in EL4 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab280353 in EL4 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

    All lanes: Immunoprecipitation - Anti-IFNGR1 antibody [EPR24127-89] (ab280353)

    Predicted band size: 54 kDa

    Observed band size: 70-95 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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