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Rabbit Recombinant Monoclonal IFNGR1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-IFNGR1 antibody [EPR25699-77] - BSA and Azide free (Capture) (AB322501), expandable thumbnail
  • Sandwich ELISA - Anti-IFNGR1 antibody [EPR25699-77] - BSA and Azide free (Capture) (AB322501), expandable thumbnail
  • Sandwich ELISA - Anti-IFNGR1 antibody [EPR25699-77] - BSA and Azide free (Capture) (AB322501), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:20015550). Associates with transmembrane accessory factor IFNGR2 to form a functional receptor (PubMed:10986460, PubMed:2971451, PubMed:7615558, PubMed:7617032, PubMed:7673114). Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to dimerization, translocation to the nucleus, and stimulation of target gene transcription (PubMed:28883123). STAT3 can also be activated in a similar manner although activation seems weaker. IFNGR1 intracellular domain phosphorylation also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1 (By similarity).

Alternative names

CD119, Interferon gamma receptor 1, IFN-gamma receptor 1, IFN-gamma-R1, CDw119, Interferon gamma receptor alpha-chain, IFN-gamma-R-alpha, IFNGR1

Rabbit Recombinant Monoclonal IFNGR1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR25699-77

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab322501 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human IFNGR1. The recommended pair for sandwich ELISA is:
Capture: ab322501, Human IFNGR1 Capture Antibody (unconjugated)
Detector: Anti-IFNGR1 antibody [EPR25699-34] - BSA and Azide free (Detector) ab322502, Human IFNGR1 Detector Antibody (unconjugated)
The reference range value is 9.7-321 pg/mL.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Anti-IFNGR1 antibody [EPR25699-77] - BSA and Azide free (Capture) (ab322501), expandable thumbnail

    Sandwich ELISA - Anti-IFNGR1 antibody [EPR25699-77] - BSA and Azide free (Capture) (ab322501)

    Interpolated concentration of IFNGR1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: HepG2 cell extract 500 µg/ml and MCF7 cell extract 500 µg/ml. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% Bovine Serum Albumin (BSA). In the MCF7 cell extract the signal was not detectable (ND). Data represented here are indicative of assay working using example of biological samples. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-IFNGR1 antibody [EPR25699-77] - BSA and Azide free (Capture) (ab322501), expandable thumbnail

    Sandwich ELISA - Anti-IFNGR1 antibody [EPR25699-77] - BSA and Azide free (Capture) (ab322501)

    Interpolated concentration of IFNGR1 was measured in duplicate at different sample concentrations. Undiluted sample is as follows: 1:2 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% Bovine Serum Albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-IFNGR1 antibody [EPR25699-77] - BSA and Azide free (Capture) (ab322501), expandable thumbnail

    Sandwich ELISA - Anti-IFNGR1 antibody [EPR25699-77] - BSA and Azide free (Capture) (ab322501)

    Sandwich ELISA of Human IFNGR1 Antibody Pair - BSA and Azide free ab322512 using human IFNGR1 immunogen protein standard curve with the capture antibody ab322501 at 2 μg/mL and detector antibody Anti-IFNGR1 antibody [EPR25699-34] - BSA and Azide free (Detector) ab322502 at 0.5 μg/mL. Background-subtracted absorbance data values (mean +/- standard deviation) are displayed.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com