Anti-IFNL4 antibody [51-1]

• BL

Supplier Data

Blocking - Anti-IFNL4 antibody [51-1] (AB196984)

Stable inducible HepG2-IFN-λ4-ISRE-Luc cells were induced with 0.5 mg/ml doxycycline for 16hours and then treated with PBS (negative control) and rabbit IgG (20μg/ml) for 22 hours and assayed for luciferase expression. α-IFN-λ4 (ab196984) blocks the ability of IFN-λ4 to induce of ISRE-Luc reporter by 70%.

This image was generated from the hybridoma version of the product.

This image is courtesy of Ludmila Prokunina-Olsson

• WB

Supplier Data

Western blot - Anti-IFNL4 antibody [51-1] (AB196984)

Prokunina-Olsson, NG, 2013 and Onabajo et al, JICR, 2015

This image was generated from the hybridoma version of the product.

All lanes:

Western blot - Anti-IFNL4 antibody [51-1] (ab196984) at 1/1500 dilution

Lane 1:

Recombinant Interferon lambda 4 protein (250 ng)

Lane 2:

Recombinant Interferon lambda 4 protein (50 ng)

Predicted band size: 20 kDa

false

This image is courtesy of Ludmila Prokunina-Olsson

• WB

Unknown

Western blot - Anti-IFNL4 antibody [51-1] (AB196984)

Blocking and dilution buffer : 5% NFDM/TBST.

The observed size is larger (50 kDa for IFNL4-Halo than the predicted size because of the Halo-tag.

All lanes:

Western blot - Anti-IFNL4 antibody [51-1] (ab196984) at 1/1000 dilution

Lane 1:

Empty vector transfected HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HepG2 transfected with Halo-tagged IFNL4, whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 20 kDa

false

• WB

CiteAb

Western blot - Anti-IFNL4 antibody [51-1] (AB196984)

IFNL4 western blot using anti-IFNL4 antibody [51-1] ab196984. Publication image and figure legend from Sung, P. S., Hong, S. H., et al., 2017, Sci Rep, PubMed 28630501.

ab196984 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab196984 please see the product overview.

IFN-λ4 overexpression induces ISG expression. (A,B) Huh-7 cells were transfected with control or IFN-λ4-expressing plasmids. After 72 hours, the cell lysates (A) and culture supernatants (B) were harvested, and protein expression were analysed by immunoblot. For the culture supernatants, immunoprecipitation with anti-FLAG-conjugated beads were used to pull down the FLAG-tagged IFN-λ4 protein. The data are representative of two independent experiments. (C,D) Huh-7 cells were transfected with control, IFN-λ1-, IFN-λ4-, or IFN-β-expressing plasmids. After 96 hours, the cells were harvested, and protein expression or gene expression were analysed by immunoblot (C) or real-time qPCR (D). (E) Huh-7 cells were transfected with IFN-λ4-expressing plasmids. The cells were harvested at the indicated time points, and gene expression was analysed by real-time qPCR. (F) Huh-7 cells were infected with JFH1 HCVcc at 0.5 MOI followed by transfection with control or IFN-λ4-expressing plasmids. After 72 hours, the cells were harvested, and HCV RNA titer was analysed by real-time qPCR. (G,H) Huh-7 cells were transfected with control, IFN-λ4-, or IFN-λ4 TT-encoding plasmids. After 96 hours, the cells were harvested, and protein and gene expression were analysed by immunoblot (G) or real-time qPCR (H). In (D,E,F,H) data are presented as means ± S.E.M. ***P ≤ 0.001 (Student’s t-test). All the data are representative of two independent experiments. Full-length blots of PY-STAT1 (C) with multiple exposure times are included in the Supplementary Fig. 9C.

false