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AB242441

Anti-IFNL4 antibody [51-1] - BSA and Azide free

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Rabbit Recombinant Monoclonal IFNL4 antibody. Carrier free. Suitable for WB, BL and reacts with Human, Synthetic peptide samples.

View Alternative Names

Interferon lambda-4, IFN-lambda-4, IFNL4

1 Images
Western blot - Anti-IFNL4 antibody [51-1] - BSA and Azide free (AB242441)
  • WB

Supplier Data

Western blot - Anti-IFNL4 antibody [51-1] - BSA and Azide free (AB242441)

Blocking and dilution buffer : 5% NFDM/TBST.

The observed size is larger (50 kDa for IFNL4-Halo than the predicted size because of the Halo-tag.

All lanes:

Western blot - Anti-IFNL4 antibody [51-1] - BSA and Azide free (ab242441) at 1/1000 dilution

Lane 1:

Empty vector transfected HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HepG2 transfected with IFNL4 with with Halo-tag, whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 20 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

51-1

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

BL, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "BL" : {"fullname" : "Blocking", "shortname":"BL"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "BL-species-checked": "testedAndGuaranteed", "BL-species-dilution-info": "", "BL-species-notes": "<p></p>" }, "Synthetic peptide": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "BL-species-checked": "notRecommended", "BL-species-dilution-info": "", "BL-species-notes": "" } } }
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Product details

ab242441 is the carrier-free version of ab196984.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IFNL4 also known as Interferon Lambda 4 plays an important role in the immune system. This protein with a mass of approximately 22 kDa is part of the type III interferon family. It expresses mainly in liver cells and epithelial tissues. IFNL4 is involved in the antiviral defense mechanism of the body. It shows activity by binding to the IFN lambda receptor complex initiating a response that helps control viral infections.
Biological function summary

IFNL4 acts as a cytokine and contributes to modulating immune responses. It is not part of a complex but interacts closely with the IFN lambda receptor to activate Janus kinase and signal transducer and activator of transcription (JAK-STAT) signaling pathways. This activation leads to the promotion of antiviral responses in infected cells and limits viral spread. Understanding its signaling function clarifies its important impact on immune defense.

Pathways

IFNL4 is fundamental to the JAK-STAT signaling pathway and to the innate immune response pathway. These pathways mediate cellular responses to viral infections. IFNL4 interacts with other proteins like IFNLR1 IL10RB and STAT1 which are essential for these pathways. By engaging these pathways IFNL4 helps control the expression of genes involved in antiviral defense such as those coding for interferon-stimulated genes (ISGs).

IFNL4 has strong connections to hepatitis C virus (HCV) infection and liver fibrosis. Researchers found that the expression of IFNL4 can influence the outcome of HCV infection affecting viral clearance and treatment response. Another related protein IFNL3 also interacts with these pathways and together they provide insights into the genetic factors influencing liver fibrosis progression. Understanding IFNL4's role provides opportunities to develop targeted therapies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that may trigger an antiviral response activating the JAK-STAT pathway and up-regulating specifically some interferon-stimulated genes.
See full target information IFNL4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com