Rabbit Polyclonal IFT122 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IFT122 aa 650-900.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human IFT122 aa 650-900. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HBG6
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes or Tris-EDTA buffer (pH 8.0) |
Target data
Function
As a component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs), it is required in ciliogenesis and ciliary protein trafficking (PubMed:27932497, PubMed:29220510). Involved in cilia formation during neuronal patterning. Acts as a negative regulator of Shh signaling. Required to recruit TULP3 to primary cilia (By similarity).
Alternative names
Intraflagellar transport protein 122 homolog, WD repeat-containing protein 10, WD repeat-containing protein 140, IFT122, SPG, WDR140, WDR10
Recommended products
Rabbit Polyclonal IFT122 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IFT122 aa 650-900.
Alternative names
Intraflagellar transport protein 122 homolog, WD repeat-containing protein 10, WD repeat-containing protein 140, IFT122, SPG, WDR140, WDR10
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human IFT122 aa 650-900. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HBG6
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab111838 is purified by antigen-affinity chromatography.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IFT122 also known as WDR10 is a subunit of the intraflagellar transport complex B (IFT-B) with a molecular mass of about 141 kDa. It participates in the assembly maintenance and function of cilia which are hair-like structures on cell surfaces that have essential roles in signaling pathways and movement. Expression of IFT122 is observed in many tissues including the kidney liver and nervous system reflecting its importance in diverse biological processes.
- Biological function summary
IFT122 plays an integral role in cilia biogenesis and function. As part of the IFT-B complex it helps to transport ciliary proteins to and from the cilia facilitating their proper assembly and function. This transport function is vital for maintaining cilia structure and signaling capabilities which are needed for various cellular responses. Proper functioning of IFT122 ensures that cilia operate effectively in transmitting signals required for cellular communication and development.
- Pathways
IFT122 is a central component in the hedgehog signaling pathway and Wnt signaling pathway. These pathways are important for embryonic development and tissue homeostasis. In the hedgehog signaling pathway IFT122 interacts with proteins like Smoothened to regulate gene expression patterns important for developmental processes. The involvement in Wnt signaling also indicates its importance in processes like cell proliferation and differentiation. Both pathways rely on functional cilia highlighting IFT122’s broader impact on cellular signaling dynamics.
- Associated diseases and disorders
Mutations or disruptions in IFT122 are linked to disorders such as cranioectodermal dysplasia (CED) and nephronophthisis. CED affects skeletal formation and tissue integrity implicating the protein's importance in developmental signaling. Nephronophthisis a genetic kidney disease shows how IFT122 dysfunction leads to cyst formation and impairments in kidney function often related to its role in ciliary assembly and signaling. Other proteins like IFT140 and IFT144 mentioned in association with IFT122 in disease states underline the interconnected nature of the intraflagellar transport system in health and disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-IFT122 antibody (ab111838)
7.5% SDS PAGE
All lanes: Western blot - Anti-IFT122 antibody (ab111838) at 1/1000 dilution
All lanes: 293T cell line lysate at 30 µg
Predicted band size: 142 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFT122 antibody (ab111838)
ab111838, at 1/500 dilution, staining IFT122 in Formalin-fixed, Paraffin-embedded Hepatoma tissue by Immunohistochemistry.
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