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AB111838

Anti-IFT122 antibody

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(1 Publication)

Rabbit Polyclonal IFT122 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IFT122 aa 650-900.

View Alternative Names

SPG, WDR10, WDR140, IFT122, Intraflagellar transport protein 122 homolog, WD repeat-containing protein 10, WD repeat-containing protein 140

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFT122 antibody (AB111838)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFT122 antibody (AB111838)

ab111838, at 1/500 dilution, staining IFT122 in Formalin-fixed, Paraffin-embedded Hepatoma tissue by Immunohistochemistry.

Western blot - Anti-IFT122 antibody (AB111838)
  • WB

Unknown

Western blot - Anti-IFT122 antibody (AB111838)

7.5% SDS PAGE

All lanes:

Western blot - Anti-IFT122 antibody (ab111838) at 1/1000 dilution

All lanes:

293T cell line lysate at 30 µg

Predicted band size: 142 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human IFT122 aa 650-900. The exact immunogen used to generate this antibody is proprietary information.

Q9HBG6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>or Tris-EDTA buffer (pH 8.0)</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab111838 is purified by antigen-affinity chromatography.
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IFT122 also known as WDR10 is a subunit of the intraflagellar transport complex B (IFT-B) with a molecular mass of about 141 kDa. It participates in the assembly maintenance and function of cilia which are hair-like structures on cell surfaces that have essential roles in signaling pathways and movement. Expression of IFT122 is observed in many tissues including the kidney liver and nervous system reflecting its importance in diverse biological processes.
Biological function summary

IFT122 plays an integral role in cilia biogenesis and function. As part of the IFT-B complex it helps to transport ciliary proteins to and from the cilia facilitating their proper assembly and function. This transport function is vital for maintaining cilia structure and signaling capabilities which are needed for various cellular responses. Proper functioning of IFT122 ensures that cilia operate effectively in transmitting signals required for cellular communication and development.

Pathways

IFT122 is a central component in the hedgehog signaling pathway and Wnt signaling pathway. These pathways are important for embryonic development and tissue homeostasis. In the hedgehog signaling pathway IFT122 interacts with proteins like Smoothened to regulate gene expression patterns important for developmental processes. The involvement in Wnt signaling also indicates its importance in processes like cell proliferation and differentiation. Both pathways rely on functional cilia highlighting IFT122’s broader impact on cellular signaling dynamics.

Mutations or disruptions in IFT122 are linked to disorders such as cranioectodermal dysplasia (CED) and nephronophthisis. CED affects skeletal formation and tissue integrity implicating the protein's importance in developmental signaling. Nephronophthisis a genetic kidney disease shows how IFT122 dysfunction leads to cyst formation and impairments in kidney function often related to its role in ciliary assembly and signaling. Other proteins like IFT140 and IFT144 mentioned in association with IFT122 in disease states underline the interconnected nature of the intraflagellar transport system in health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As a component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs), it is required in ciliogenesis and ciliary protein trafficking (PubMed : 27932497, PubMed : 29220510). Involved in cilia formation during neuronal patterning. Acts as a negative regulator of Shh signaling. Required to recruit TULP3 to primary cilia (By similarity).
See full target information IFT122
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 9:224 PubMed40615519

2025

Genetic and oncogenic features of RASGRF fusions.

Applications

Unspecified application

Species

Unspecified reactive species

Sreya Das,Daniel S Lenchner,Ellen Jaeger,Lisa Hunihan,Dana F DeSantis,Stamatina Fragkogianni,Karyn Ronski,Frederick H Wilson
View all publications
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Product promise

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