AB42497

Anti-IFT88 antibody

Name: Anti-IFT88 antibody (ab42497) | Abcam
Brand: Abcam Limited
SKU: AB42497
Price: 545 USD
Availability: InStock
Rating: 3 (2 reviews)

(2 Reviews)

(11 Publications)

Goat Polyclonal IFT88 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human IFT88 aa 800 to C-terminus.

View Alternative Names

TG737, TTC10, IFT88, Intraflagellar transport protein 88 homolog, Recessive polycystic kidney disease protein Tg737 homolog, Tetratricopeptide repeat protein 10, TPR repeat protein 10

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-IFT88 antibody (AB42497)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IFT88 antibody (AB42497)

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells labeling IFT88 with ab42497 (green). Cells permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (2μg/ml) showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10μg/ml) followed by Alexa Fluor® 488 secondary antibody (2μg/ml).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IFT88 antibody (AB42497)

Immunofluorescence analysis of paraformaldehyde fixed A549 cells labeling IFT88 with ab42497 (green). Cells permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (2μg/ml) showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10μg/ml) followed by Alexa Fluor® 488 secondary antibody (2μg/ml).

Flow Cytometry (Intracellular) - Anti-IFT88 antibody (AB42497)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IFT88 antibody (AB42497)

Flow cytometric analysis of paraformaldehyde fixed THP-1 cells (blue line) permeabilized with 0.5% Triton. Primary incubation 1hr (10μg/ml) followed by Alexa Fluor® 488 conjugated secondary antibody (1μg/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.

Supplier Data

Western blot - Anti-IFT88 antibody (AB42497)

Lysates, 35µg protein in RIPA buffer. Detected by chemiluminescence.

All lanes:

Western blot - Anti-IFT88 antibody (ab42497) at 2 µg/mL

Lane 1:

K562 (human chronic myeloid leukemia (CML) cell line) cell lysate at 35 µg

Lane 2:

THP-1 (human monocytic leukemia cell line) cell lysate at 35 µg

Lane 3:

U251 (human glioblastoma multiforme cell line) cell lysate at 35 µg

Lane 4:

KNRK (Kirsten murine sarcoma virus-transformed normal rat kidney cells) cell lysate at 35 µg

Observed band size: 80 kDa,85 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human IFT88 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13099

Specificity

This antibody is expected to recognise both reported isoforms (NP_783195.2; NP_006522.2).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IFT88 also known as Intraflagellar transport protein 88 is an essential component of the intraflagellar transport (IFT) mechanism. This protein has a mass of approximately 88 kDa and is expressed in ciliated cells across various tissues. Cilia are critical structures in cellular function and IFT88 operates as part of the IFT-B complex playing a role in the assembly and maintenance of cilia. Its localization primarily occurs in the cilia where it functions as a transporter of protein cargos necessary for cilia construction and function.

Biological function summary

The role of IFT88 within the context of cilia involves the facilitation of bidirectional movement along the axoneme which is the central shaft of a cilium. As part of the IFT-B complex IFT88 aids in the transport of tubulin and other ciliary components from the cell body to the ciliary tip ensuring cilia's structural integrity and functional viability. Additionally IFT88 supports signal transduction processes associated with ciliary functions including sensing extracellular signals and maintaining cellular signaling pathways.

Pathways

IFT88 has significant involvement in the Hedgehog and Wnt signaling pathways. These pathways are integral for cell differentiation proliferation and embryonic development. IFT88 collaborates with proteins such as Gli transcription factors in Hedgehog signaling and interacts with various cytosolic components in Wnt signaling showing its versatile role in cellular processes. Through these interactions IFT88 indirectly influences the outcome of these signaling pathways impacting tissue patterning and cell fate decisions during development.

IFT88 mutations are linked to ciliopathies like polycystic kidney disease and Bardet-Biedl syndrome. These conditions demonstrate defects in ciliary function where IFT88 dysfunction results in abnormal cellular processes. The connection between IFT88 and these diseases extends to its interactions with proteins such as polycystin-1 and polycystin-2 in kidney development and function. Such associations emphasize the critical role of IFT88 in maintaining normal cellular and organ function whereby its impairment leads to profound developmental and physiological anomalies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Positively regulates primary cilium biogenesis (PubMed : 17604723). Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.

See full target information IFT88

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 36:109461 PubMed34348137

2021

Repeated nuclear translocations underlie photoreceptor positioning and lamination of the outer nuclear layer in the mammalian retina.

Applications

Unspecified application

Species

Unspecified reactive species

Nozie D Aghaizu,Katherine M Warre-Cornish,Martha R Robinson,Paul V Waldron,Ryea N Maswood,Alexander J Smith,Robin R Ali,Rachael A Pearson

Current biology : CB 29:3323-3330.e8 PubMed31564489

2019

Planar Cell Polarity Effector Proteins Inturned and Fuzzy Form a Rab23 GEF Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Gerondopoulos,Helen Strutt,Nicola L Stevenson,Tomoaki Sobajima,Tim P Levine,David J Stephens,David Strutt,Francis A Barr

Nature communications 10:4072 PubMed31492868

2019

Ciliary exclusion of Polycystin-2 promotes kidney cystogenesis in an autosomal dominant polycystic kidney disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca V Walker,Jennifer L Keynton,Daniel T Grimes,Vrinda Sreekumar,Debbie J Williams,Chris Esapa,Dongsheng Wu,Martin M Knight,Dominic P Norris

Molecular biology of the cell 30:828-837 PubMed30759057

2019

Single-particle tracking localization microscopy reveals nonaxonemal dynamics of intraflagellar transport proteins at the base of mammalian primary cilia.

Applications

Unspecified application

Species

Unspecified reactive species

T Tony Yang,Minh Nguyet Thi Tran,Weng Man Chong,Chia-En Huang,Jung-Chi Liao

Biophysical journal 115:263-275 PubMed29866362

2018

Super-Resolution Imaging Reveals TCTN2 Depletion-Induced IFT88 Lumen Leakage and Ciliary Weakening.

Applications

Unspecified application

Species

Unspecified reactive species

Rueyhung Roc Weng,T Tony Yang,Chia-En Huang,Chih-Wei Chang,Won-Jing Wang,Jung-Chi Liao

The Journal of cell biology 216:1287-1300 PubMed28385950

2017

The daughter centriole controls ciliogenesis by regulating Neurl-4 localization at the centrosome.

Applications

Unspecified application

Species

Unspecified reactive species

Abdelhalim Loukil,Kati Tormanen,Christine Sütterlin

Developmental biology 409:55-71 PubMed26542012

2015

Primary cilia are critical for Sonic hedgehog-mediated dopaminergic neurogenesis in the embryonic midbrain.

Applications

Unspecified application

Species

Unspecified reactive species

Mary Gazea,Evangelia Tasouri,Marianna Tolve,Viktoria Bosch,Anna Kabanova,Christian Gojak,Bahtiyar Kurtulmus,Orna Novikov,Joachim Spatz,Gislene Pereira,Wolfgang Hübner,Claude Brodski,Kerry L Tucker,Sandra Blaess

Development (Cambridge, England) 141:3966-77 PubMed25294941

2014

ATMIN is a transcriptional regulator of both lung morphogenesis and ciliogenesis.

Applications

Species

Mouse

Paraskevi Goggolidou,Jonathan L Stevens,Francesco Agueci,Jennifer Keynton,Gabrielle Wheway,Daniel T Grimes,Saloni H Patel,Helen Hilton,Stine K Morthorst,Antonella DiPaolo,Debbie J Williams,Jeremy Sanderson,Svetlana V Khoronenkova,Nicola Powles-Glover,Alexander Ermakov,Chris T Esapa,Rosario Romero,Grigory L Dianov,James Briscoe,Colin A Johnson,Lotte B Pedersen,Dominic P Norris

PloS one 8:e62554 PubMed23690943

2013

Primary cilia: the chemical antenna regulating human adipose-derived stem cell osteogenesis.

Applications

ICC/IF

Species

Human

Josephine C Bodle,Candace D Rubenstein,Michelle E Phillips,Susan H Bernacki,Jie Qi,Albert J Banes,Elizabeth G Loboa

Cilia 1:23 PubMed23351706

2012

Multiple essential roles for primary cilia in heart development.

Applications

IHC

Species

Unspecified reactive species

Marc August Willaredt,Karin Gorgas,Humphrey A R Gardner,Kerry L Tucker

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IFT88 antibody

Immunocytochemistry/ Immunofluorescence - Anti-IFT88 antibody (AB42497)

Immunocytochemistry/ Immunofluorescence - Anti-IFT88 antibody (AB42497)

Flow Cytometry (Intracellular) - Anti-IFT88 antibody (AB42497)

Western blot - Anti-IFT88 antibody (AB42497)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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