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AB250767

Anti-IFT88 antibody [EPR14850] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal IFT88 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TG737, TTC10, IFT88, Intraflagellar transport protein 88 homolog, Recessive polycystic kidney disease protein Tg737 homolog, Tetratricopeptide repeat protein 10, TPR repeat protein 10

3 Images
Flow Cytometry (Intracellular) - Anti-IFT88 antibody [EPR14850] - BSA and Azide free (AB250767)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IFT88 antibody [EPR14850] - BSA and Azide free (AB250767)

This data was developed using ab184566, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of HepG2 cells (2% paraformaldehyde-fixed) labeling IFT88 with ab184566 at 1/260 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Western blot - Anti-IFT88 antibody [EPR14850] - BSA and Azide free (AB250767)
  • WB

Supplier Data

Western blot - Anti-IFT88 antibody [EPR14850] - BSA and Azide free (AB250767)

This data was developed using ab184566, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-IFT88 antibody [EPR14850] - N-terminal (<a href='/en-us/products/primary-antibodies/ift88-antibody-epr14850-n-terminal-ab184566'>ab184566</a>) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 94 kDa

false

Western blot - Anti-IFT88 antibody [EPR14850] - BSA and Azide free (AB250767)
  • WB

Supplier Data

Western blot - Anti-IFT88 antibody [EPR14850] - BSA and Azide free (AB250767)

This data was developed using ab184566, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-IFT88 antibody [EPR14850] - N-terminal (<a href='/en-us/products/primary-antibodies/ift88-antibody-epr14850-n-terminal-ab184566'>ab184566</a>) at 1/50000 dilution

Lane 1:

293 cell lysate at 20 µg

Lane 2:

Human fetal kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa,94 kDa,96 kDa

Observed band size: 105 kDa,51 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14850

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250767 is the carrier-free version of ab184566.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IFT88 also known as Intraflagellar transport protein 88 is an essential component of the intraflagellar transport (IFT) mechanism. This protein has a mass of approximately 88 kDa and is expressed in ciliated cells across various tissues. Cilia are critical structures in cellular function and IFT88 operates as part of the IFT-B complex playing a role in the assembly and maintenance of cilia. Its localization primarily occurs in the cilia where it functions as a transporter of protein cargos necessary for cilia construction and function.
Biological function summary

The role of IFT88 within the context of cilia involves the facilitation of bidirectional movement along the axoneme which is the central shaft of a cilium. As part of the IFT-B complex IFT88 aids in the transport of tubulin and other ciliary components from the cell body to the ciliary tip ensuring cilia's structural integrity and functional viability. Additionally IFT88 supports signal transduction processes associated with ciliary functions including sensing extracellular signals and maintaining cellular signaling pathways.

Pathways

IFT88 has significant involvement in the Hedgehog and Wnt signaling pathways. These pathways are integral for cell differentiation proliferation and embryonic development. IFT88 collaborates with proteins such as Gli transcription factors in Hedgehog signaling and interacts with various cytosolic components in Wnt signaling showing its versatile role in cellular processes. Through these interactions IFT88 indirectly influences the outcome of these signaling pathways impacting tissue patterning and cell fate decisions during development.

IFT88 mutations are linked to ciliopathies like polycystic kidney disease and Bardet-Biedl syndrome. These conditions demonstrate defects in ciliary function where IFT88 dysfunction results in abnormal cellular processes. The connection between IFT88 and these diseases extends to its interactions with proteins such as polycystin-1 and polycystin-2 in kidney development and function. Such associations emphasize the critical role of IFT88 in maintaining normal cellular and organ function whereby its impairment leads to profound developmental and physiological anomalies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Positively regulates primary cilium biogenesis (PubMed : 17604723). Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.
See full target information IFT88
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 16:4716-4727 PubMed39678812

2024

Enhancing the radiosensitivity of colorectal cancer cells by reducing spermine synthase through promoting autophagy and DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Bin Guo,Yue-Ming Wu,Zhi-Zhao Lin
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com