AB124716

Anti-IgA antibody [EPR5367-76]

Name: Anti-IgA antibody [EPR5367-76] (ab124716) | Abcam
Brand: Abcam Limited
SKU: AB124716
Price: 5885 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(14 Publications)

Anti-IgA antibody [EPR5367-76] (ab124716) is a rabbit monoclonal antibody detecting IgA in Western Blot, IP, IHC-P, IHC-Fr, ELISA. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Immunoglobulin heavy constant alpha 1, Ig alpha-1 chain C region, Ig alpha-1 chain C region BUR, Ig alpha-1 chain C region TRO, IGHA1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (AB124716)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (AB124716)

ab124716, at 1/100, staining IgA in paraffin embedded Human colon tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (AB124716)

ab124716, at 1/100, staining IgA in paraffin embedded Human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-IgA antibody [EPR5367-76] (AB124716)

Capture ab 1 : 60 dilution (1μg in 1mg lysate). Primary ab for WB ab124716 at 1 : 1000 dilution (0.18μg/ml). Secondary ab Anti-Rabbit IgG (HRP), specific to the non5% NFDM/TBST -reduced form of IgG. Secondary ab concentration 1 : 1500 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-IgA antibody [EPR5367-76] (ab124716) at 1/1000 dilution

Lane 1:

Human plasma

Lane 2:

ab124716 at 1/30 IP in Human plasma

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab124716 in Human plasma

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

Predicted band size: 83 kDa

Observed band size: 60 kDa

false

Unknown

Western blot - Anti-IgA antibody [EPR5367-76] (AB124716)

All lanes:

Western blot - Anti-IgA antibody [EPR5367-76] (ab124716) at 1/1000 dilution

Lane 1:

Human tonsil lysate at 10 µg

Lane 2:

Human plasma lysate at 10 µg

Lane 3:

Human spleen lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 83 kDa

false

Supplier Data

Western blot - Anti-IgA antibody [EPR5367-76] (AB124716)

This antibody does not cross-react with human IgA2.

In Western blot, Anti-IgA antibody [EPR5367-76] - (ab124716) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-IgA1 antibody [EPR26775-27] (<a href='/en-us/products/primary-antibodies/iga1-antibody-epr26775-27-ab316187'>ab316187</a>) at 1/1000 dilution

Lane 1:

Native Human IgA1 protein (<a href='/en-us/products/proteins-peptides/native-human-iga1-protein-ab91020'>ab91020</a>) at 10 ng

Lane 2:

Native Human IgA2 protein (<a href='/en-us/products/proteins-peptides/native-human-iga2-protein-ab91021'>ab91021</a>) at 10 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 60 kDa

false

Exposure time: 59s

Carrier free

Anti-IgA antibody [EPR5367-76] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-IgA antibody [EPR5367-76]
Carrier free

Anti-IgA antibody [EPR5367-76] - BSA and Azide free (Capture/Detector)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5367-76

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ELISA, IHC-P, IP, IHC-Fr, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody reacts with Heavy chain of IgA. It does not react with either kappa or lambda light chain. This antibody does not cross-react with human IgA2. It does not react with other Ig.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/250 - 1/4000", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-IgA antibody [EPR5367-76] (ab124716) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), ELISA in Human samples.

What is the molecular weight of IgA?
Anti-IgA [EPR5367-76] (ab124716) specifically detects a band for IgA (UniProt: P01877) at a molecular weight of 37kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR5367-76] also available for your convenience: ab124716, Carrier free - ab214003, Alexa Fluor® 488 - ab223410, Carrier free - ab244893

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Immunoglobulin A (IgA) also known as sIgA in its secretory form is an important component of the immune system. With a molecular weight of approximately 160 kDa IgA exists as a monomeric or dimeric structure. It is primarily expressed in mucosal areas such as the respiratory tract gastrointestinal tract and urogenital tract while also being present in secretions like saliva tears and breast milk. In its dimeric form IgA associates with a joining (J) chain and a secretory component which facilitates its transport across mucosal barriers.

Biological function summary

IgA functions as a first line of defense in immune responses occurring at mucosal surfaces. Its primary role is to neutralize pathogens toxins and prevent their attachment and penetration through epithelial cells. This antibody does not form part of a larger complex but plays an important role in immune exclusion by trapping antigens in the mucus layer. Additionally IgA can mediate antibody-dependent cellular cytotoxicity and engage with specific receptors like FcαRI (CD89) on immune cells further enhancing its protective capabilities.

Pathways

IgA participates in the mucosal immune response which is a sub-component of the broader immune system pathways. It is closely associated with the polymeric immunoglobulin receptor (pIgR) which transports IgA to the mucosal surfaces. The pathway involves recombination activation proteins (RAG) during its class-switching process in B cells differentiating from IgM-producing cells to those secreting IgA. These pathways are integral to maintaining the mucosal environment and protecting against pathogens.

Alterations or deficiencies in IgA can lead to increased susceptibility to infections and disorders like IgA nephropathy and celiac disease. In IgA nephropathy IgA deposits in the kidneys result in inflammation and damage to renal tissues. Celiac disease involves an immune reaction to gluten where IgA antibodies against tissue transglutaminase (tTG) contribute to intestinal damage. Both conditions illustrate the importance of IgA in maintaining immune balance and its connection with disease pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed : 2241915).

See full target information IGHA1

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:7784 PubMed39237503

2024

Unraveling the spatial organization and development of human thymocytes through integration of spatial transcriptomics and single-cell multi-omics profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Yanchuan Li,Huamei Li,Cheng Peng,Ge Meng,Yijun Lu,Honglin Liu,Li Cui,Huan Zhou,Zhu Xu,Lingyun Sun,Lihong Liu,Qing Xiong,Beicheng Sun,Shiping Jiao

eLife 12: PubMed38055614

2023

Discovery and characterization of cross-reactive intrahepatic antibodies in severe alcoholic hepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Reza Ahmadi,Guang Song,Tianshun Gao,Jing Ma,Xiaomei Han,Ming-Wen Hu,Andrew M Cameron,Russell N Wesson,Benjamin Philosophe,Shane Ottmann,Elizabeth King,Ahmet Gurakar,Le Qi,Brandon Peiffer,James Burdick,Robert Anders,Zhanxiang Zhou,Hongkun Lu,Dechun Feng,Chien-Sheng Chen,Jiang Qian,Bin Gao,Heng Zhu,Zhaoli Sun

Immunity 56:2570-2583.e6 PubMed37909039

2023

Targeting intracellular oncoproteins with dimeric IgA promotes expulsion from the cytoplasm and immune-mediated control of epithelial cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Subir Biswas,Gunjan Mandal,Carmen M Anadon,Ricardo A Chaurio,Luis U Lopez-Bailon,Mate Z Nagy,Jessica A Mine,Kay Hänggi,Kimberly B Sprenger,Patrick Innamarato,Carly M Harro,John J Powers,Joseph Johnson,Bin Fang,Mostafa Eysha,Xiaolin Nan,Roger Li,Bradford A Perez,Tyler J Curiel,Xiaoqing Yu,Paulo C Rodriguez,Jose R Conejo-Garcia

Frontiers in immunology 14:1184167 PubMed37675100

2023

Integration of single-cell RNA sequencing and bulk RNA transcriptome sequencing reveals a heterogeneous immune landscape and pivotal cell subpopulations associated with colorectal cancer prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Zhang,Yang Liu,Xinyu Wang,Cheng Zhang,Mingxiao Hou,Yunen Liu

Cellular and molecular gastroenterology and hepatology 15:393-424 PubMed36216310

2022

Single-cell Transcriptomics Reveals Early Molecular and Immune Alterations Underlying the Serrated Neoplasia Pathway Toward Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Jie Zhou,Xiao-Fan Lu,Huimin Chen,Xin-Yuan Wang,Wenxuan Cheng,Qing-Wei Zhang,Jin-Nan Chen,Xiao-Yi Wang,Jing-Zheng Jin,Fang-Rong Yan,Haoyan Chen,Xiao-Bo Li

JCI insight 7: PubMed36134664

2022

Single-cell transcriptome mapping identifies a local, innate B cell population driving chronic rejection after lung transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia F Smirnova,Kent Riemondy,Marta Bueno,Susan Collins,Pavan Suresh,Xingan Wang,Kapil N Patel,Carlyne Cool,Melanie Königshoff,Nirmal S Sharma,Oliver Eickelberg

Molecules (Basel, Switzerland) 27: PubMed35807530

2022

High Expression Level of α2-3-Linked Sialic Acids on Salivary Glycoproteins of Breastfeeding Women May Help to Protect Them from Avian Influenza Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Li Ding,Yimin Cheng,Wei Guo,Siyue Sun,Xiangqin Chen,Tiantian Zhang,Hongwei Cheng,Jiayue Hao,Yunhua Lu,Xiurong Wang,Zheng Li

Cancer research 82:859-871 PubMed34949671

2021

IgA-Dominated Humoral Immune Responses Govern Patients' Outcome in Endometrial Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Gunjan Mandal,Subir Biswas,Carmen M Anadon,Xiaoqing Yu,Chandler D Gatenbee,Sandhya Prabhakaran,Kyle K Payne,Ricardo A Chaurio,Alexandra Martin,Patrick Innamarato,Carlos Moran,John J Powers,Carly M Harro,Jessica A Mine,Kimberly B Sprenger,Kristen E Rigolizzo,Xuefeng Wang,Tyler J Curiel,Paulo C Rodriguez,Alexander R Anderson,Ozlen Saglam,Jose R Conejo-Garcia

medRxiv : the preprint server for health sciences : PubMed34268518

2021

Next-generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael D Melani,Benjamin J Des Soye,Jared O Kafader,Eleonora Forte,Michael Hollas,Voislav Blagojevic,Fernanda Negrão,John P McGee,Bryon Drown,Cameron Lloyd-Jones,Henrique S Seckler,Jeannie M Camarillo,Philip D Compton,Richard D LeDuc,Bryan Early,Ryan T Fellers,Byoung-Kyu Cho,Basil Baby Mattamana,Young Ah Goo,Paul M Thomas,Michelle K Ash,Pavan P Bhimalli,Lena Al-Harthi,Beverly E Sha,Jeffrey R Schneider,Neil L Kelleher

Glycoconjugate journal 38:55-65 PubMed33608772

2021

Aberrant RL2 O-GlcNAc antibody reactivity against serum-IgA1 of patients with colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chris Verathamjamras,Tanin-Ek Sriwitool,Pukkavadee Netsirisawan,Parunya Chaiyawat,Daranee Chokchaichamnankit,Naiyarat Prasongsook,Chantragan Srisomsap,Jisnuson Svasti,Voraratt Champattanachai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IgA antibody [EPR5367-76]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (AB124716)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (AB124716)

Immunoprecipitation - Anti-IgA antibody [EPR5367-76] (AB124716)

Western blot - Anti-IgA antibody [EPR5367-76] (AB124716)

Western blot - Anti-IgA antibody [EPR5367-76] (AB124716)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise