AB214003

Anti-IgA antibody [EPR5367-76] - BSA and Azide free

Name: Anti-IgA antibody [EPR5367-76] - Carrier free (ab214003) | Abcam
Brand: Abcam Limited
SKU: AB214003
Price: 905 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(6 Publications)

Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting IgA in Western Blot, IP, IHC-P, IHC-Fr, ELISA. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Immunoglobulin heavy constant alpha 1, Ig alpha-1 chain C region, Ig alpha-1 chain C region BUR, Ig alpha-1 chain C region TRO, IGHA1

4 Images

Immunohistochemistry (Frozen sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (AB214003)

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (AB214003)

Clone EPR5367-76 (ab214003) has been successfully conjugated by Abcam. This image was generated using Anti-IgA antibody [EPR5367-76] (Alexa Fluor® 488). Please refer to ab223410 for protocol details.

IHC image of IgA staining in a section of frozen normal human tonsil*.

The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab223410 at 1/5000 dilution (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (AB214003)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (AB214003)

ab124716, at 1/100, staining IgA in paraffin embedded Human colon tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124716).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (AB214003)

ab124716, at 1/100, staining IgA in paraffin embedded Human tonsil tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124716).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (AB214003)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124716).

All lanes:

Immunoprecipitation - Anti-IgA antibody [EPR5367-76] (<a href='/en-us/products/primary-antibodies/iga-antibody-epr5367-76-ab124716'>ab124716</a>) at 1/1000 dilution

Lane 1:

Human plasma

Lane 2:

<a href='/en-us/products/primary-antibodies/iga-antibody-epr5367-76-ab124716'>ab124716</a> at 1/30 IP in Human plasma

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/iga-antibody-epr5367-76-ab124716'>ab124716</a> in Human plasma

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

Predicted band size: 83 kDa

Observed band size: 60 kDa

false

Unconjugated

Anti-IgA antibody [EPR5367-76]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-IgA antibody [EPR5367-76]
Carrier free

Anti-IgA antibody [EPR5367-76] - BSA and Azide free (Capture/Detector)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5367-76

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, IHC-Fr, IHC-P, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody reacts with Heavy chain of IgA. It does not react with either kappa or lambda light chain. This antibody does not cross-react with human IgA2. It does not react with other Ig.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), ELISA in Human samples.

What is the molecular weight of IgA?
Anti-IgA [EPR5367-76] - BSA and Azide free (ab214003) specifically detects a band for IgA (UniProt: P01877) at a molecular weight of 37kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR5367-76] also available for your convenience: ab124716, Carrier free - ab214003, Alexa Fluor® 488 - ab223410, Carrier free - ab244893

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Immunoglobulin A (IgA) also known as sIgA in its secretory form is an important component of the immune system. With a molecular weight of approximately 160 kDa IgA exists as a monomeric or dimeric structure. It is primarily expressed in mucosal areas such as the respiratory tract gastrointestinal tract and urogenital tract while also being present in secretions like saliva tears and breast milk. In its dimeric form IgA associates with a joining (J) chain and a secretory component which facilitates its transport across mucosal barriers.

Biological function summary

IgA functions as a first line of defense in immune responses occurring at mucosal surfaces. Its primary role is to neutralize pathogens toxins and prevent their attachment and penetration through epithelial cells. This antibody does not form part of a larger complex but plays an important role in immune exclusion by trapping antigens in the mucus layer. Additionally IgA can mediate antibody-dependent cellular cytotoxicity and engage with specific receptors like FcαRI (CD89) on immune cells further enhancing its protective capabilities.

Pathways

IgA participates in the mucosal immune response which is a sub-component of the broader immune system pathways. It is closely associated with the polymeric immunoglobulin receptor (pIgR) which transports IgA to the mucosal surfaces. The pathway involves recombination activation proteins (RAG) during its class-switching process in B cells differentiating from IgM-producing cells to those secreting IgA. These pathways are integral to maintaining the mucosal environment and protecting against pathogens.

Alterations or deficiencies in IgA can lead to increased susceptibility to infections and disorders like IgA nephropathy and celiac disease. In IgA nephropathy IgA deposits in the kidneys result in inflammation and damage to renal tissues. Celiac disease involves an immune reaction to gluten where IgA antibodies against tissue transglutaminase (tTG) contribute to intestinal damage. Both conditions illustrate the importance of IgA in maintaining immune balance and its connection with disease pathologies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed : 2241915).

See full target information IGHA1

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Pediatric blood & cancer 70:e30473 PubMed37249415

2023

Humoral profiling of pediatric patients with cancer reveals robust immunity following anti-SARS-CoV-2 vaccination superior to natural infection.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha D Martin,Nadège Nziza,Pietro Miozzo,Yannic Bartsch,Eva J Farkas,Abigail S Kane,Lauren H Boal,Alison Friedmann,Galit Alter,Lael M Yonker

Frontiers in microbiology 13:893801 PubMed35707164

2022

Harmonization of Multiple SARS-CoV-2 Reference Materials Using the WHO IS (NIBSC 20/136): Results and Implications.

Applications

Unspecified application

Species

Unspecified reactive species

William Jonathan Windsor,Yannik Roell,Heidi Tucker,Chi-An Cheng,Sara Suliman,Laura J Peek,Gary A Pestano,William T Lee,Heinz Zeichhardt,Molly M Lamb,Martin Kammel,Hui Wang,Ross Kedl,Cody Rester,Thomas E Morrison,Bennet J Davenport,Kyle Carson,Jennifer Yates,Kelly Howard,Karen Kulas,David R Walt,Aner Dafni,Daniel Taylor,May Chu

The Journal of clinical investigation 131: PubMed34032635

2021

Multisystem inflammatory syndrome in children is driven by zonulin-dependent loss of gut mucosal barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Lael M Yonker,Tal Gilboa,Alana F Ogata,Yasmeen Senussi,Roey Lazarovits,Brittany P Boribong,Yannic C Bartsch,Maggie Loiselle,Magali Noval Rivas,Rebecca A Porritt,Rosiane Lima,Jameson P Davis,Eva J Farkas,Madeleine D Burns,Nicola Young,Vinay S Mahajan,Soroush Hajizadeh,Xcanda I Herrera Lopez,Johannes Kreuzer,Robert Morris,Enid E Martinez,Isaac Han,Kettner Griswold,Nicholas C Barry,David B Thompson,George Church,Andrea G Edlow,Wilhelm Haas,Shiv Pillai,Moshe Arditi,Galit Alter,David R Walt,Alessio Fasano

Nature biomedical engineering : PubMed32948854

2020

Ultrasensitive high-resolution profiling of early seroconversion in patients with COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Maia Norman,Tal Gilboa,Alana F Ogata,Adam M Maley,Limor Cohen,Evan L Busch,Roey Lazarovits,Chih-Ping Mao,Yongfei Cai,Jun Zhang,Jared E Feldman,Blake M Hauser,Timothy M Caradonna,Bing Chen,Aaron G Schmidt,Galit Alter,Richelle C Charles,Edward T Ryan,David R Walt

medRxiv : the preprint server for health sciences : PubMed32511657

2020

Ultra-Sensitive High-Resolution Profiling of Anti-SARS-CoV-2 Antibodies for Detecting Early Seroconversion in COVID-19 Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Maia Norman,Tal Gilboa,Alana F Ogata,Adam M Maley,Limor Cohen,Yongfei Cai,Jun Zhang,Jared E Feldman,Blake M Hauser,Timothy M Caradonna,Bing Chen,Aaron G Schmidt,Galit Alter,Richelle C Charles,Edward T Ryan,David R Walt

PloS one 8:e76176 PubMed24098437

2013

Differential binding of IgG and IgA to mucus of the female reproductive tract.

Applications

Species

Human

Kelly M Fahrbach,Olga Malykhina,Daniel J Stieh,Thomas J Hope

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IgA antibody [EPR5367-76] - BSA and Azide free

Immunohistochemistry (Frozen sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (AB214003)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (AB214003)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (AB214003)

Immunoprecipitation - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (AB214003)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise