Mouse Monoclonal IgA Secretory Component antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.
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![Western blot - Anti-IgA Secretory Component antibody [SC-05] (AB3924), expandable thumbnail](https://content.abcam.com/products/images/iga-secretory-component-antibody-sc-05-ab3924--western-blot-img96143.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IgA Secretory Component antibody [SC-05] (AB3924)")
Publications
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- Cell reports. Medicine 2:1004682021Secretory IgA and T cells targeting SARS-CoV-2 spike protein are transferred to the breastmilk upon mRNA vaccination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuliana Gonçalves,A Margarida Juliano,Nádia Charepe,Marta Alenquer,Diogo Athayde,Filipe Ferreira,Margarida Archer,Maria João Amorim,Fátima Serrano,Helena SoaresPubMed 34873588
- PloS one 16:e02499792021Detection of cross-reactive immunoglobulin A against the severe acute respiratory syndrome-coronavirus-2 spike 1 subunit in saliva.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeiichi Tsukinoki,Tatsuo Yamamoto,Keisuke Handa,Mariko Iwamiya,Juri Saruta,Satoshi Ino,Takashi SakuraiPubMed 34813596
- Nature 591:464-4702021IgA transcytosis and antigen recognition govern ovarian cancer immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSubir Biswas,Gunjan Mandal,Kyle K Payne,Carmen M Anadon,Chandler D Gatenbee,Ricardo A Chaurio,Tara Lee Costich,Carlos Moran,Carly M Harro,Kristen E Rigolizzo,Jessica A Mine,Jimena Trillo-Tinoco,Naoko Sasamoto,Kathryn L Terry,Douglas Marchion,Andrea Buras,Robert M Wenham,Xiaoqing Yu,Mary K Townsend,Shelley S Tworoger,Paulo C Rodriguez,Alexander R Anderson,Jose R Conejo-GarciaPubMed 33536615
- Cell reports 30:1292-1299.e32020Human Fc Receptor-like 3 Inhibits Regulatory T Cell Function and Binds Secretory IgA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStuti Agarwal,Zachary Kraus,Jessica Dement-Brown,Oyeleye Alabi,Kyle Starost,Mate TolnayPubMed 32023449
- American journal of respiratory and critical care medicine 184:317-272011Bronchial secretory immunoglobulin a deficiency correlates with airway inflammation and progression of chronic obstructive pulmonary disease.Applications:IHC-PReactive species:HumanVasiliy V Polosukhin,Justin M Cates,William E Lawson,Rinat Zaynagetdinov,Aaron P Milstone,Pierre P Massion,Sebahat Ocak,Lorraine B Ware,Jae Woo Lee,Russell P Bowler,Alexey V Kononov,Scott H Randell,Timothy S BlackwellPubMed 21512171
- Histochemistry 91:235-441989Secretory component in differentiating normal epithelium, benign lesions and malignancy in the human breast as monitored by monoclonal antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Bártek,H Tlaskalová-Hogenová,Z Stasková,J Simecková,B Vojtĕsek,A Rejthar,J Kovarík,J BartkováPubMed 2722565
- International journal of cancer 42:638-411988Rapid selection of cultured cells with increased expression of a membrane marker (secretory component).Applications:Unspecified applicationReactive species:Unspecified reactive speciesD Kvale,J Bartek,L M Sollid,P BrandtzaegPubMed 2459074
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes See Abreview. Use under non-reducing conditions. |
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Mouse Monoclonal IgA Secretory Component antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- SC-05
- Purification technique
- Affinity purification Protein A
- Specificity
ab3924 reacts with 80 kDa human secretory component glycoprotein (both free and bound in secretory IgA). The recognized glycoprotein is a specific membrane marker of glandular carcinomas.
- Concentration
- Purification notes
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The IgA Secretory Component often referred to as IgA SC or Anti-Secretory is an important segment of the polymeric immunoglobulin receptor (pIgR) with a mass around 83 kDa. It is primarily expressed on epithelial cells where it plays a role in mucosal immunity. This component facilitates the transcytosis of polymeric IgA antibodies across epithelial layers and plays an important role in the secretion of IgA into mucosal networks. Due to its mechanism of action the Secretory Component ensures that IgA antibodies reach the mucosal surface where they help in neutralizing pathogens and toxins.
- Biological function summary
The IgA Secretory Component is essential in forming a complex with polymeric IgA to create Secretory IgA (SIgA). This complex is integral to mucosal surfaces such as the respiratory gastrointestinal and urogenital tracts providing a defense mechanism against environmental pathogens. The association between IgA SC and polymeric IgA confers stability and protection to the IgA dimers from proteolytic enzymes ensuring their functional role in local immunological responses at mucosal sites.
- Pathways
IgA Secretory Component is involved in pathways for mucosal immunity and IgA antibody transport. It interacts with proteins related to the immune response including J-chain which binds to the polymeric IgA facilitating its binding to the pIgR. This interaction denotes a critical step in the active transport and subsequent secretion of SIgA at epithelial surfaces which are vital in immunological defense mechanisms.
- Associated diseases and disorders
IgA Secretory Component is linked to conditions such as selective IgA deficiency and inflammatory bowel disease (IBD). In selective IgA deficiency where there is a lack or reduced levels of IgA dysfunction in IgA SC may disrupt mucosal immunity. In inflammatory bowel disease improper regulation or expression of the IgA SC plays a role impacting the protective SIgA along the gut mucosa. Such conditions show connections to other proteins within the immune system like cytokines that influence inflammatory responses and immune regulation at the mucosal barriers.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Western blot - Anti-IgA Secretory Component antibody [SC-05] (ab3924), expandable thumbnail](https://content.abcam.com/products/images/iga-secretory-component-antibody-sc-05-ab3924--western-blot-img96143.jpg "Western blot - Anti-IgA Secretory Component antibody [SC-05] (ab3924)")
Western blot - Anti-IgA Secretory Component antibody [SC-05] (ab3924)
Secretory Component Glycoprotein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
All lanes: Western blot - Anti-IgA Secretory Component antibody [SC-05] (ab3924) at 1 µg/mL
Lane 1: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 38 kDa, 68 kDa
Exposure time: 20min
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