AB3924

Anti-IgA Secretory Component antibody [SC-05]

Name: Anti-IgA Secretory Component antibody [SC-05] (ab3924) | Abcam
Brand: Abcam Limited
SKU: AB3924
Price: 565 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(8 Publications)

Anti-IgA Secretory Component antibody [SC-05] (ab3924) is a mouse monoclonal antibody detecting IgA Secretory Component in Western Blot. Suitable for Human.

- Over 10 publications
- Trusted since 2003

1 Images

Unknown

Western blot - Anti-IgA Secretory Component antibody [SC-05] (AB3924)

Secretory Component Glycoprotein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-IgA Secretory Component antibody [SC-05] (ab3924) at 1 µg/mL

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 61 kDa

Observed band size: 38 kDa,68 kDa

true

Exposure time: 20min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SC-05

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

applications

Specificity

ab3924 reacts with 80 kDa human secretory component glycoprotein (both free and bound in secretory IgA). The recognized glycoprotein is a specific membrane marker of glandular carcinomas.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>See Abreview. Use under non-reducing conditions.</p>" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-IgA Secretory Component antibody [SC-05] (ab3924) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of IgA Secretory Component?
Anti-IgA Secretory Component [SC-05] (ab3924) specifically detects a band for IgA Secretory Component (UniProt: Q16610) at a molecular weight of 61kDa.

Trusted by the scientific community
Anti-IgA Secretory Component [SC-05] (ab3924) was first used in a scientific publication in 2003 and has been cited over 10 times in peer-reviewed journals.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IgA Secretory Component often referred to as IgA SC or Anti-Secretory is an important segment of the polymeric immunoglobulin receptor (pIgR) with a mass around 83 kDa. It is primarily expressed on epithelial cells where it plays a role in mucosal immunity. This component facilitates the transcytosis of polymeric IgA antibodies across epithelial layers and plays an important role in the secretion of IgA into mucosal networks. Due to its mechanism of action the Secretory Component ensures that IgA antibodies reach the mucosal surface where they help in neutralizing pathogens and toxins.

Biological function summary

The IgA Secretory Component is essential in forming a complex with polymeric IgA to create Secretory IgA (SIgA). This complex is integral to mucosal surfaces such as the respiratory gastrointestinal and urogenital tracts providing a defense mechanism against environmental pathogens. The association between IgA SC and polymeric IgA confers stability and protection to the IgA dimers from proteolytic enzymes ensuring their functional role in local immunological responses at mucosal sites.

Pathways

IgA Secretory Component is involved in pathways for mucosal immunity and IgA antibody transport. It interacts with proteins related to the immune response including J-chain which binds to the polymeric IgA facilitating its binding to the pIgR. This interaction denotes a critical step in the active transport and subsequent secretion of SIgA at epithelial surfaces which are vital in immunological defense mechanisms.

IgA Secretory Component is linked to conditions such as selective IgA deficiency and inflammatory bowel disease (IBD). In selective IgA deficiency where there is a lack or reduced levels of IgA dysfunction in IgA SC may disrupt mucosal immunity. In inflammatory bowel disease improper regulation or expression of the IgA SC plays a role impacting the protective SIgA along the gut mucosa. Such conditions show connections to other proteins within the immune system like cytokines that influence inflammatory responses and immune regulation at the mucosal barriers.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information IgA Secretory Component

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Immunity 56:2570-2583.e6 PubMed37909039

2023

Targeting intracellular oncoproteins with dimeric IgA promotes expulsion from the cytoplasm and immune-mediated control of epithelial cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Subir Biswas,Gunjan Mandal,Carmen M Anadon,Ricardo A Chaurio,Luis U Lopez-Bailon,Mate Z Nagy,Jessica A Mine,Kay Hänggi,Kimberly B Sprenger,Patrick Innamarato,Carly M Harro,John J Powers,Joseph Johnson,Bin Fang,Mostafa Eysha,Xiaolin Nan,Roger Li,Bradford A Perez,Tyler J Curiel,Xiaoqing Yu,Paulo C Rodriguez,Jose R Conejo-Garcia

Cell reports. Medicine 2:100468 PubMed34873588

2021

Secretory IgA and T cells targeting SARS-CoV-2 spike protein are transferred to the breastmilk upon mRNA vaccination.

Applications

Unspecified application

Species

Unspecified reactive species

Juliana Gonçalves,A Margarida Juliano,Nádia Charepe,Marta Alenquer,Diogo Athayde,Filipe Ferreira,Margarida Archer,Maria João Amorim,Fátima Serrano,Helena Soares

PloS one 16:e0249979 PubMed34813596

2021

Detection of cross-reactive immunoglobulin A against the severe acute respiratory syndrome-coronavirus-2 spike 1 subunit in saliva.

Applications

Unspecified application

Species

Unspecified reactive species

Keiichi Tsukinoki,Tatsuo Yamamoto,Keisuke Handa,Mariko Iwamiya,Juri Saruta,Satoshi Ino,Takashi Sakurai

Nature 591:464-470 PubMed33536615

2021

IgA transcytosis and antigen recognition govern ovarian cancer immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Subir Biswas,Gunjan Mandal,Kyle K Payne,Carmen M Anadon,Chandler D Gatenbee,Ricardo A Chaurio,Tara Lee Costich,Carlos Moran,Carly M Harro,Kristen E Rigolizzo,Jessica A Mine,Jimena Trillo-Tinoco,Naoko Sasamoto,Kathryn L Terry,Douglas Marchion,Andrea Buras,Robert M Wenham,Xiaoqing Yu,Mary K Townsend,Shelley S Tworoger,Paulo C Rodriguez,Alexander R Anderson,Jose R Conejo-Garcia

Cell reports 30:1292-1299.e3 PubMed32023449

2020

Human Fc Receptor-like 3 Inhibits Regulatory T Cell Function and Binds Secretory IgA.

Applications

Unspecified application

Species

Unspecified reactive species

Stuti Agarwal,Zachary Kraus,Jessica Dement-Brown,Oyeleye Alabi,Kyle Starost,Mate Tolnay

American journal of respiratory and critical care medicine 184:317-27 PubMed21512171

2011

Bronchial secretory immunoglobulin a deficiency correlates with airway inflammation and progression of chronic obstructive pulmonary disease.

Applications

IHC-P

Species

Human

Vasiliy V Polosukhin,Justin M Cates,William E Lawson,Rinat Zaynagetdinov,Aaron P Milstone,Pierre P Massion,Sebahat Ocak,Lorraine B Ware,Jae Woo Lee,Russell P Bowler,Alexey V Kononov,Scott H Randell,Timothy S Blackwell

Histochemistry 91:235-44 PubMed2722565

1989

Secretory component in differentiating normal epithelium, benign lesions and malignancy in the human breast as monitored by monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

J Bártek,H Tlaskalová-Hogenová,Z Stasková,J Simecková,B Vojtĕsek,A Rejthar,J Kovarík,J Bartková

International journal of cancer 42:638-41 PubMed2459074

1988

Rapid selection of cultured cells with increased expression of a membrane marker (secretory component).

Applications

Unspecified application

Species

Unspecified reactive species

D Kvale,J Bartek,L M Sollid,P Brandtzaeg

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IgA Secretory Component antibody [SC-05]

Western blot - Anti-IgA Secretory Component antibody [SC-05] (AB3924)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise