AB212330

Anti-IgA Secretory Component antibody [SPM217] - BSA and Azide free

Name: Anti-IgA Secretory Component antibody [SPM217] - BSA and Azide free (ab212330) | Abcam
Brand: Abcam Limited
SKU: AB212330

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(3 Publications)

Mouse Monoclonal IgA Secretory Component antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications.

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA Secretory Component antibody [SPM217] - BSA and Azide free (AB212330)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA Secretory Component antibody [SPM217] - BSA and Azide free (AB212330)

This image was produced using ab17921, the same clone but in a different formulation.

Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon carcinoma tissue labeling IgA Secretory Component with ab17921 at 1 µg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SPM217

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Specificity

ab212330 reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IgA Secretory Component often referred to as IgA SC or Anti-Secretory is an important segment of the polymeric immunoglobulin receptor (pIgR) with a mass around 83 kDa. It is primarily expressed on epithelial cells where it plays a role in mucosal immunity. This component facilitates the transcytosis of polymeric IgA antibodies across epithelial layers and plays an important role in the secretion of IgA into mucosal networks. Due to its mechanism of action the Secretory Component ensures that IgA antibodies reach the mucosal surface where they help in neutralizing pathogens and toxins.

Biological function summary

The IgA Secretory Component is essential in forming a complex with polymeric IgA to create Secretory IgA (SIgA). This complex is integral to mucosal surfaces such as the respiratory gastrointestinal and urogenital tracts providing a defense mechanism against environmental pathogens. The association between IgA SC and polymeric IgA confers stability and protection to the IgA dimers from proteolytic enzymes ensuring their functional role in local immunological responses at mucosal sites.

Pathways

IgA Secretory Component is involved in pathways for mucosal immunity and IgA antibody transport. It interacts with proteins related to the immune response including J-chain which binds to the polymeric IgA facilitating its binding to the pIgR. This interaction denotes a critical step in the active transport and subsequent secretion of SIgA at epithelial surfaces which are vital in immunological defense mechanisms.

IgA Secretory Component is linked to conditions such as selective IgA deficiency and inflammatory bowel disease (IBD). In selective IgA deficiency where there is a lack or reduced levels of IgA dysfunction in IgA SC may disrupt mucosal immunity. In inflammatory bowel disease improper regulation or expression of the IgA SC plays a role impacting the protective SIgA along the gut mucosa. Such conditions show connections to other proteins within the immune system like cytokines that influence inflammatory responses and immune regulation at the mucosal barriers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information IgA Secretory Component

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Gut microbes 14:2143220 PubMed36369946

2022

Kidney microbiota dysbiosis contributes to the development of hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yu Liu,Jing Li,Yamei Zhang,Luyun Fan,Yanli Xia,Yongyang Wu,Junru Chen,Xinyu Zhao,Qiannan Gao,Bing Xu,Chunlai Nie,Zhengyu Li,Aiping Tong,Wenjie Wang,Jun Cai

BioMed research international 2022:4801695 PubMed35837380

2022

Intervention of Shugan Xiaozhi Decoction on Nonalcoholic Fatty Liver Disease via Mediating Gut-Liver Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Huili Yang,Lian Feng,Linyi Xu,Dansheng Jiang,Fenfen Zhai,Guangdong Tong,Yufeng Xing

The Journal of allergy and clinical immunology 139:1966-1978.e9 PubMed27993536

2016

Neutrophils are a major source of the epithelial barrier disrupting cytokine oncostatin M in patients with mucosal airways disease.

Applications

Unspecified application

Species

Unspecified reactive species

Kathryn L Pothoven,James E Norton,Lydia A Suh,Roderick G Carter,Kathleen E Harris,Assel Biyasheva,Kevin Welch,Stephanie Shintani-Smith,David B Conley,Mark C Liu,Atsushi Kato,Pedro C Avila,Qutayba Hamid,Leslie C Grammer,Anju T Peters,Robert C Kern,Bruce K Tan,Robert P Schleimer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IgA Secretory Component antibody [SPM217] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA Secretory Component antibody [SPM217] - BSA and Azide free (AB212330)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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