Anti-IgA1 antibody [EPR26775-27]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA1 antibody [EPR26775-27] (AB316187)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling IgA1 with ab316187 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human skeletal muscle. The section was incubated with ab316187 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA1 antibody [EPR26775-27] (AB316187)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling IgA1 with ab316187 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on immune cells of human tonsil. The section was incubated with ab316187 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA1 antibody [EPR26775-27] (AB316187)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling IgA1 with ab316187 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on plasma and immune cells of human colon. The section was incubated with ab316187 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA1 antibody [EPR26775-27] (AB316187)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling IgA1 with ab316187 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on plasma of human kidney. The section was incubated with ab316187 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-IgA1 antibody [EPR26775-27] (AB316187)

Low expression : human heart, human skeletal muscle.

The identity of the lower MW band at approximately 32 kDa (in lane 4) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-IgA1 antibody [EPR26775-27] (ab316187) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Human heart tissue lysate at 20 µg

Lane 4:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 60 kDa,36 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-IgA1 antibody [EPR26775-27] (AB316187)

All lanes:

Western blot - Anti-IgA1 antibody [EPR26775-27] (ab316187) at 1/1000 dilution

Lane 1:

Human serum at 20 µg

Lane 2:

Human plasma at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 60 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-IgA1 antibody [EPR26775-27] (AB316187)

This antibody does not cross-react with human IgA2.

In Western blot, Anti-IgA antibody [EPR5367-76] - (ab124716) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-IgA1 antibody [EPR26775-27] (ab316187) at 1/1000 dilution

Lane 1:

Native Human IgA1 protein (<a href='/en-us/products/proteins-peptides/native-human-iga1-protein-ab91020'>ab91020</a>) at 10 ng

Lane 2:

Native Human IgA2 protein (<a href='/en-us/products/proteins-peptides/native-human-iga2-protein-ab91021'>ab91021</a>) at 10 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 60 kDa

false

Exposure time: 59s