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Rabbit Recombinant Monoclonal IGHA1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Purified native protein - Human, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (AB316188), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (AB316188), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (AB316188), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (AB316188), expandable thumbnail
  • Western blot - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (AB316188), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIPFlow CytICC/IF
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Purified native protein - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Purified native protein - Human, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Purified native protein - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Purified native protein - Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Purified native protein - Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Purified native protein - Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed:2241915).

Alternative names

Immunoglobulin heavy constant alpha 1, Ig alpha-1 chain C region, Ig alpha-1 chain C region BUR, Ig alpha-1 chain C region TRO, IGHA1

Recommended products

Rabbit Recombinant Monoclonal IGHA1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Purified native protein - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26775-27
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab316188 is the carrirer-free version of Anti-IgA1 antibody [EPR26775-27] ab316187.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

IgA1 also known as Immunoglobulin A1 is an important component of the immune system. This protein mainly exists in mucous membranes where it plays an important role in immune function. IgA1 has a mass of approximately 160 kDa and is expressed in various tissues including the respiratory and gastrointestinal tracts. IgA1 is a subclass of immunoglobulin A (IgA) and is characterized by its heavy chain constant region which distinguishes it from other immunoglobulin subclasses.

Biological function summary

IgA1 acts as a first line of defense in the immune system by binding to pathogens and neutralizing them. It exists as a monomer or forms a dimer with the help of the J chain which stabilizes its dimeric form. IgA1 also interacts with the polymeric immunoglobulin receptor (pIgR) for transportation across epithelial cells. This transport allows IgA1 to be present in secretory fluids such as saliva and breast milk providing an important protective function.

Pathways

IgA1 interacts with several pathways involved in immune response and mucosal immunity. One critical pathway is the mucosal antibody response where IgA1 plays a role in preventing infection at mucosal surfaces. The IgA1 protein activates the alternative complement pathway by binding to complement protein C3 which enhances the removal of pathogens. Furthermore IgA1 is associated with the J chain and pIgR in the process of IgA transcytosis across epithelial barriers.

Associated diseases and disorders

IgA1 has strong connections to IgA nephropathy and celiac disease. In IgA nephropathy IgA1 deposits in glomeruli leading to inflammation and kidney damage. The increased serum levels of IgA1 often with aberrant glycosylation contribute to disease development. In celiac disease IgA1 reacts with tissue transglutaminase causing an immune response that damages the small intestine's lining. In both disorders the interactions between IgA1 and other proteins such as tissue transglutaminase in celiac disease highlight its involvement in pathological processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (ab316188), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (ab316188)

    This data was developed using Anti-IgA1 antibody [EPR26775-27] ab316187, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling IgA1 with Anti-IgA1 antibody [EPR26775-27] ab316187 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human skeletal muscle. The section was incubated with Anti-IgA1 antibody [EPR26775-27] ab316187 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (ab316188), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (ab316188)

    This data was developed using Anti-IgA1 antibody [EPR26775-27] ab316187, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling IgA1 with Anti-IgA1 antibody [EPR26775-27] ab316187 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on plasma and immune cells of human colon. The section was incubated with Anti-IgA1 antibody [EPR26775-27] ab316187 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (ab316188), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (ab316188)

    This data was developed using Anti-IgA1 antibody [EPR26775-27] ab316187, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling IgA1 with Anti-IgA1 antibody [EPR26775-27] ab316187 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on immune cells of human tonsil. The section was incubated with Anti-IgA1 antibody [EPR26775-27] ab316187 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (ab316188), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (ab316188)

    This data was developed using Anti-IgA1 antibody [EPR26775-27] ab316187, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling IgA1 with Anti-IgA1 antibody [EPR26775-27] ab316187 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on plasma of human kidney. The section was incubated with Anti-IgA1 antibody [EPR26775-27] ab316187 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (ab316188), expandable thumbnail

    Western blot - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (ab316188)

    This data was developed using Anti-IgA1 antibody [EPR26775-27] ab316187, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-IgA1 antibody [EPR26775-27] (Anti-IgA1 antibody [EPR26775-27] ab316187) at 1/1000 dilution

    Lane 1: Human serum at 20 µg with 5% NFDM/TBST

    Lane 2: Human plasma at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 60 kDa

    Exposure time: 59s

  • Western blot - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (ab316188), expandable thumbnail

    Western blot - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (ab316188)

    This data was developed using Anti-IgA1 antibody [EPR26775-27] ab316187, the same antibody clone in a different buffer formulation.

    This antibody does not cross-react with human IgA2.

    In Western blot, Anti-IgA antibody [EPR5367-76] - (Anti-IgA antibody [EPR5367-76] ab124716) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-IgA1 antibody [EPR26775-27] (Anti-IgA1 antibody [EPR26775-27] ab316187) at 1/1000 dilution

    Lane 1: Native Human IgA1 protein (Native Human IgA1 protein ab91020) at 10 ng with 5% NFDM/TBST

    Lane 2: Native Human IgA2 protein (Native Human IgA2 protein ab91021) at 10 ng with 5% NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 60 kDa

    Exposure time: 59s

  • Western blot - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (ab316188), expandable thumbnail

    Western blot - Anti-IgA1 antibody [EPR26775-27] - BSA and Azide free (ab316188)

    This data was developed using Anti-IgA1 antibody [EPR26775-27] ab316187, the same antibody clone in a different buffer formulation.

    Low expression: human heart, human skeletal muscle.

    The identity of the lower MW band at approximately 32 kDa (in lane 4) is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-IgA1 antibody [EPR26775-27] (Anti-IgA1 antibody [EPR26775-27] ab316187) at 1/1000 dilution

    Lane 1: Human colon tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Human kidney tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 3: Human heart tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 4: Human skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 60 kDa, 36 kDa

    Exposure time: 59s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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