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AB312874

Anti-IgA1 antibody [EPR26775-52] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal IGHA1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Immunoglobulin heavy constant alpha 1, Ig alpha-1 chain C region, Ig alpha-1 chain C region BUR, Ig alpha-1 chain C region TRO, IGHA1

1 Images
Sandwich ELISA - Anti-IgA1 antibody [EPR26775-52] - BSA and Azide free (Detector) (AB312874)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-IgA1 antibody [EPR26775-52] - BSA and Azide free (Detector) (AB312874)

Sandwich ELISA of ab312872 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26775-52

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IgA1 also known as Immunoglobulin A1 is an important component of the immune system. This protein mainly exists in mucous membranes where it plays an important role in immune function. IgA1 has a mass of approximately 160 kDa and is expressed in various tissues including the respiratory and gastrointestinal tracts. IgA1 is a subclass of immunoglobulin A (IgA) and is characterized by its heavy chain constant region which distinguishes it from other immunoglobulin subclasses.
Biological function summary

IgA1 acts as a first line of defense in the immune system by binding to pathogens and neutralizing them. It exists as a monomer or forms a dimer with the help of the J chain which stabilizes its dimeric form. IgA1 also interacts with the polymeric immunoglobulin receptor (pIgR) for transportation across epithelial cells. This transport allows IgA1 to be present in secretory fluids such as saliva and breast milk providing an important protective function.

Pathways

IgA1 interacts with several pathways involved in immune response and mucosal immunity. One critical pathway is the mucosal antibody response where IgA1 plays a role in preventing infection at mucosal surfaces. The IgA1 protein activates the alternative complement pathway by binding to complement protein C3 which enhances the removal of pathogens. Furthermore IgA1 is associated with the J chain and pIgR in the process of IgA transcytosis across epithelial barriers.

IgA1 has strong connections to IgA nephropathy and celiac disease. In IgA nephropathy IgA1 deposits in glomeruli leading to inflammation and kidney damage. The increased serum levels of IgA1 often with aberrant glycosylation contribute to disease development. In celiac disease IgA1 reacts with tissue transglutaminase causing an immune response that damages the small intestine's lining. In both disorders the interactions between IgA1 and other proteins such as tissue transglutaminase in celiac disease highlight its involvement in pathological processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed : 2241915).
See full target information IGHA1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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