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AB170911

Anti-IGBP1 antibody [EPR12315]

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(2 Publications)

Rabbit Recombinant Monoclonal IGBP1 antibody. Suitable for WB, ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

IBP1, IGBP1, Immunoglobulin-binding protein 1, B-cell signal transduction molecule alpha 4, CD79a-binding protein 1, Protein phosphatase 2/4/6 regulatory subunit, Renal carcinoma antigen NY-REN-16, Protein alpha-4

5 Images
Immunohistochemistry paraffin embedded sections - Anti-IGBP1 antibody [EPR12315] (AB170911)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-IGBP1 antibody [EPR12315] (AB170911)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling IGBP1 using ab170911 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-IGBP1 antibody [EPR12315] (AB170911)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IGBP1 antibody [EPR12315] (AB170911)

Intracellular flow cytometric analysis of permeabilized K562 cells labeling IGBP1 using ab170911 at 1/100 dilution (red)or a rabbit IgG negative (green).

Immunohistochemistry paraffin embedded sections - Anti-IGBP1 antibody [EPR12315] (AB170911)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-IGBP1 antibody [EPR12315] (AB170911)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling IGBP1 using ab170911 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-IGBP1 antibody [EPR12315] (AB170911)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IGBP1 antibody [EPR12315] (AB170911)

Immunofluorescent staining of HeLa cells labeling IGBP1 using ab170911 at 1/100 dilution (red). DAPI nuclear staining (blue).

Western blot - Anti-IGBP1 antibody [EPR12315] (AB170911)
  • WB

Supplier Data

Western blot - Anti-IGBP1 antibody [EPR12315] (AB170911)

All lanes:

Western blot - Anti-IGBP1 antibody [EPR12315] (ab170911) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Raji cell lysate at 10 µg

Predicted band size: 39 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12315

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry paraffin embedded sections", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Insulin-like Growth Factor Binding Protein 1 (IGBP1) is a multifunctional protein involved in various cellular processes. It is also known as IGFBP-1 and has a molecular mass of approximately 31 kDa. IGBP1 is widely expressed in human tissues with higher levels detected in the liver and the endometrium. The protein plays a role in modulating the availability of insulin-like growth factors (IGFs) by binding to them affecting their function and stability within the body.
Biological function summary

Many cellular processes involve IGBP1 as it binds with insulin-like growth factors to modulate their actions effectively acting as a regulator within the IGF system. IGBP1 may influence cell growth survival and metabolism. It often interacts with other proteins within an IGF-binding complex allowing it to perform various regulatory functions that impact both normal and pathological conditions.

Pathways

Several signaling pathways incorporate the activities of IGBP1 particularly the insulin-like growth factor signaling pathway and the PI3K/AKT pathway. IGBP1 interacts with other proteins such as IGF1R and IRS-1 affecting downstream signaling that governs aspects of cell proliferation and survival. These interactions position IGBP1 as an important regulatory molecule in these pathways influencing cellular responses to IGFs.

Research has linked abnormal levels or activities of IGBP1 with conditions such as diabetes and cancer. IGBP1's modulation of the insulin-like growth factors may affect insulin sensitivity and glucose metabolism contributing to diabetes pathogenesis. In cancer altered IGBP1 expression can influence tumor growth and progression where its relationship with IGF1 and IGF2 is significant in the context of growth factors and their signaling pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Associated to surface IgM-receptor; may be involved in signal transduction. Involved in regulation of the catalytic activity of the phosphatases PP2A, PP4 and PP6 by protecting their partially folded catalytic subunits from degradative polyubiquitination until they associate with regulatory subunits.
See full target information IGBP1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7815 PubMed36535970

2022

Targeting the transcription factor HES1 by L-menthol restores protein phosphatase 6 in keratinocytes in models of psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhikai Wang,Yang Sun,Fangzhou Lou,Jing Bai,Hong Zhou,Xiaojie Cai,Libo Sun,Qianqian Yin,Sibei Tang,Yue Wu,Li Fan,Zhenyao Xu,Hong Wang,Xiaoyu Hu,Honglin Wang

Nature communications 9:3659 PubMed30201971

2018

ER-associated ubiquitin ligase HRD1 programs liver metabolism by targeting multiple metabolic enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Juncheng Wei,Yanzhi Yuan,Lu Chen,Yuanming Xu,Yuehui Zhang,Yajun Wang,Yanjie Yang,Clara Bien Peek,Lauren Diebold,Yi Yang,Beixue Gao,Chaozhi Jin,Johanna Melo-Cardenas,Navdeep S Chandel,Donna D Zhang,Hui Pan,Kezhong Zhang,Jian Wang,Fuchu He,Deyu Fang
View all publications
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