Rat Monoclonal IgD antibody. Carrier free. Suitable for Flow Cyt, IHC-Fr and reacts with Mouse samples.
Images
![Flow Cytometry - Anti-IgD antibody [11-26] - BSA and Azide free (AB235126), expandable thumbnail](https://content.abcam.com/products/images/igd-antibody-11-26-bsa-and-azide-free-ab235126--flow-cytometry-img119702.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-IgD antibody [11-26] - BSA and Azide free (AB235126)")
![Immunohistochemistry (Frozen sections) - Anti-IgD antibody [11-26] - BSA and Azide free (AB235126), expandable thumbnail](https://content.abcam.com/products/images/igd-antibody-11-26-bsa-and-azide-free-ab235126--immunohistochemistry-frozen-sections-img38492.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-IgD antibody [11-26] - BSA and Azide free (AB235126)")
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | IHC-Fr | |
|---|---|---|
| Mouse | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.1 µg/mL | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes This product gave a positive signal in HeLa cells fixed with 10% formaldehyde (10 min). |
Associated Products
Select an associated product type
2 products for Alternative Product
1 product for Alternative Version
Target data
Alternative names
Ig delta chain C region, Immunoglobulin heavy constant delta
Recommended products
Rat Monoclonal IgD antibody. Carrier free. Suitable for Flow Cyt, IHC-Fr and reacts with Mouse samples.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 11-26
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Do Not Freeze
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IgD also known as Immunoglobulin D serves as a distinct antibody isotype expressed primarily on the surface of B cells. It is involved in the initiation of immune responses. IgD has a molecular weight of approximately 180 kDa. The full form of IgD is Immunoglobulin D. It is expressed mainly in the respiratory system the tonsils and lymphoid tissues playing a pivotal role in the early stages of immunity. Researchers often perform IgD tests to study its mechanical aspects and influence on immune cell functionality.
- Biological function summary
IgD contributes to the activation and differentiation of B lymphocytes and may influence antibody affinity maturation. This immunoglobulin is not known to form part of a larger complex but acts in conjunction with other immunoglobulins like IgM to regulate immune responses. The presence of IgD on B cells suggests its involvement in signaling processes which help B cells transition from immature to fully functional states impacting how the immune system responds to pathogens.
- Pathways
IgD plays roles in immune system signaling and pathogen recognition. It appears in pathways such as B cell receptor signaling and participates in complex interactions with other proteins such as IgM and CD79 which are essential for transmitting activation signals. These pathways are important enabling the immune system to identify and respond to antigens effectively offering a first line of defense against infections.
- Associated diseases and disorders
IgD has associations with recurrent respiratory infections and IgD myeloma a rare form of plasma cell neoplasm. The malfunction or dysregulation of IgD can impact B cell development and lead to immune system disorders. In such cases IgD levels often measured through IgD tests may serve as important indicators for monitoring disease progression. Moreover elevated levels of IgD can coexist with proteins such as IgE particularly in allergy-related responses and play roles in certain immunodeficiency conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-IgD antibody [11-26] - BSA and Azide free (ab235126), expandable thumbnail](https://content.abcam.com/products/images/igd-antibody-11-26-bsa-and-azide-free-ab235126--flow-cytometry-img119702.jpg "Flow Cytometry - Anti-IgD antibody [11-26] - BSA and Azide free (ab235126)")
Flow Cytometry - Anti-IgD antibody [11-26] - BSA and Azide free (ab235126)
C57BL/6 mouse splenocytes stained with ab235126 (right) or rat IgG2aκ (left). C57BL/6 Mouse splenocytes were incubated for 30 min on ice in 10% mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab235126) or rat IgG2aκ Isotype (Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450) (1x106 in 100μl at 0.1 μg/ml) for 30 min on ice.
The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165) was used at 1/2000 dilution for 30 min at 4°C.The cells were simultaneously stained with CD19 antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
![Immunohistochemistry (Frozen sections) - Anti-IgD antibody [11-26] - BSA and Azide free (ab235126), expandable thumbnail](https://content.abcam.com/products/images/igd-antibody-11-26-bsa-and-azide-free-ab235126--immunohistochemistry-frozen-sections-img38492.jpg "Immunohistochemistry (Frozen sections) - Anti-IgD antibody [11-26] - BSA and Azide free (ab235126)")
Immunohistochemistry (Frozen sections) - Anti-IgD antibody [11-26] - BSA and Azide free (ab235126)
IHC image of IgD staining in a section of frozen normal mouse spleen*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab235126 (1µg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed ab150167 (Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 647) preabsorbed) (shown in red) and DAPI (staining nuclear DNA)(shown in blue) for 1 hour at room temperature . The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab235126.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from Charles River.
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