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AB106836

Anti-IGF1 antibody

4

(3 Reviews)

|

(19 Publications)

Goat Polyclonal IGF1 antibody. Suitable for ICC, WB, IHC-P and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human IGF1 aa 100-150.

View Alternative Names

IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF

2 Images
Immunocytochemistry - Anti-IGF1 antibody (AB106836)
  • ICC

Supplier Data

Immunocytochemistry - Anti-IGF1 antibody (AB106836)

Immunocytochemistry analysis of paraformaldehyde fixed HEK-293 (human epithelial cell line from embryonic kidney) cells, permeabilized with 0.15% Triton. Primary incubation for 1 hour at 10 μg/mL followed by Alexa Fluor® 488 secondary antibody at 2 μg/mL. The nuclear stain is DAPI (blue).

Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (2 μg/mL).

Western blot - Anti-IGF1 antibody (AB106836)
  • WB

Supplier Data

Western blot - Anti-IGF1 antibody (AB106836)

Primary incubation for 1 hour at room temperature. Detected by chemiluminescence.

All lanes:

Western blot - Anti-IGF1 antibody (ab106836) at 0.5 µg/mL

Lane 1:

Human uterus tissue lysate in RIPA buffer at 35 µg

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate in RIPA buffer at 35 µg

Predicted band size: 22 kDa

Observed band size: 15 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human IGF1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P05019

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The insulin-like growth factor 1 or IGF1 is a protein hormone that plays an important mechanical role in growth and development. Alternative names for IGF1 include somatomedin C. This protein has a mass of approximately 7.6 kDa and is mainly produced by the liver. IGF1 is expressed in a range of tissues including muscle bone and cartilage reflecting its importance in various physiological processes. Its production is stimulated by growth hormone and it acts in an autocrine and paracrine manner to exert its effects.
Biological function summary

IGF1 stimulates growth and proliferation of cells most notably impacting the development of the skeletal system and the regulation of apoptosis. It is not part of a larger protein complex but it interacts closely with IGF1 receptor to execute its functions. By binding to this receptor IGF1 activates intracellular signaling cascades that promote anabolic effects which include increased uptake of glucose and amino acids contributing to muscle and tissue growth.

Pathways

IGF1 operates within the PI3K-Akt and MAPK signaling pathways which are significant for cell survival and proliferation. Its interaction with proteins like IGF binding protein 3 (IGFBP3) modulates its availability and activity in the bloodstream. These pathways are critical for mediating the effects of growth hormone highlighting the role of IGF1 in systemic growth and metabolism regulation.

Alterations in IGF1 levels associate with growth abnormalities such as Laron syndrome and acromegaly. Laron syndrome results from the body's inability to use IGF1 properly due to receptor defects while acromegaly occurs from excessive IGF1 production often due to pituitary tumors. Furthermore aberrant IGF1 signaling connects to cancer development where it can drive tumor growth and metastasis. Drugs targeting IGF1 and its pathway components continue to be researched for therapeutic potential in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed : 21076856, PubMed : 24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV : ITGB3 and ITGA6 : ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed : 19578119, PubMed : 22351760, PubMed : 23243309, PubMed : 23696648). As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).
See full target information IGF1
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 15:62 PubMed40399921

2025

Tie2-expressing monocytes/macrophages promote angiogenesis in chronically ischaemic brain tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Chuyang Tai,Cong Ling,Yang Yang,Baoyu Zhang,Jun Sun,Ni Mo,Tao Sun,Lixin Huang,Cian Yao,Hui Wang,Chuan Chen

Stem cell research & therapy 15:75 PubMed38475906

2024

Menstrual blood-derived mesenchymal stem cells combined with collagen I gel as a regenerative therapeutic strategy for degenerated disc after discectomy in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yu,Honghao Wu,Shumei Zeng,Xiaojian Hu,Yuxu Wu,Jinhong Zhou,Li Yuan,Qingqing Zhang,Charlie Xiang,Zhiyun Feng

Investigative ophthalmology & visual science 65:1 PubMed38441891

2024

Alteration of Neurotrophic Factors and Innervation in Extraocular Muscles of Individuals With Concomitant Esotropia.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Hao,Meixu Wang,Jiawen Liu,Mayinuer Yusufu,Kai Cao,Jing Fu

Immunity, inflammation and disease 12:e1098 PubMed38270302

2024

The Lnc-ENST00000602558/IGF1 axis as a predictor of response to treatment with tripterygium glycosides in rheumatoid arthritis patients.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Gao,Xiaoyue Wang,Yanfeng Gao,Jian Bai,Yanpeng Zhao,Renyi Wang,Hanzhou Wang,Guangzhao Zhu,Xixi Wang,Xiaochen Han,Yanqiong Zhang,Hailong Wang

Science advances 9:eadg0666 PubMed37531435

2023

Local autocrine plasticity signaling in single dendritic spines by insulin-like growth factors.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Tu,Anant Jain,Paula Parra Bueno,Helena Decker,Xiaodan Liu,Ryohei Yasuda

Nutrition research and practice 16:419-434 PubMed35919286

2022

Ameliorative effect of Nakai on benign prostatic hyperplasia and .

Applications

Unspecified application

Species

Unspecified reactive species

Young-Jin Choi,Meiqi Fan,Yujiao Tang,Sangho Moon,Seung-Hyun Lee,Bokyung Lee,Sung Mun Bae,Sang Moo Lee,Eun-Kyung Kim

Frontiers in endocrinology 13:879962 PubMed35966057

2022

Sexual dimorphic effects of deficiency on metabolism in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Ningmei Zeng,Jiankang Bao,TingTing Shu,Chuang Shi,Gang Zhai,Xia Jin,Jiangyan He,Qiyong Lou,Zhan Yin

Cell cycle (Georgetown, Tex.) 21:1980-1995 PubMed35575588

2022

miR-29a-3p mitigates the development of osteosarcoma through modulating IGF1 mediated PI3k/Akt/FOXO3 pathway by activating autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Song Qi,Li Xu,Yongyuan Han,Hongkun Chen,Anyuan Cheng

Stem cell research & therapy 13:19 PubMed35033199

2022

Engineered adipose-derived stem cells with IGF-1-modified mRNA ameliorates osteoarthritis development.

Applications

Unspecified application

Species

Unspecified reactive species

Haoyu Wu,Zhi Peng,Ying Xu,Zixuan Sheng,Yanshan Liu,Youguo Liao,Yin Wang,Ya Wen,Junzhi Yi,Chang Xie,Xuri Chen,Jiajie Hu,Bingqian Yan,Huijing Wang,Xudong Yao,Wei Fu,Hongwei Ouyang

Annals of translational medicine 9:1376 PubMed34733928

2021

TNF-α-stimulated nucleus pulposus cells induce cell apoptosis through the release of exosomal miR-16 targeting IGF-1 and IGF-1R in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Chen Zhang,Yan-Pei Zou,Shun-Qi Hu,Tai-Wei Zhang,Hao Zhou,Bing Liang,Chen-Yang Zhuang,Hui-Ren Wang,Li-Bo Jiang,Xi-Lei Li
View all publications
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