Goat Polyclonal IGF1 antibody. Suitable for ICC, WB, IHC-P and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human IGF1 aa 100-150.
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- Nutrition research and practice 16:419-4342022Ameliorative effect of Nakai on benign prostatic hyperplasia and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoung-Jin Choi,Meiqi Fan,Yujiao Tang,Sangho Moon,Seung-Hyun Lee,Bokyung Lee,Sung Mun Bae,Sang Moo Lee,Eun-Kyung KimPubMed 35919286
- Frontiers in endocrinology 13:8799622022Sexual dimorphic effects of deficiency on metabolism in zebrafish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNingmei Zeng,Jiankang Bao,TingTing Shu,Chuang Shi,Gang Zhai,Xia Jin,Jiangyan He,Qiyong Lou,Zhan YinPubMed 35966057
- Stem cell research & therapy 13:192022Engineered adipose-derived stem cells with IGF-1-modified mRNA ameliorates osteoarthritis development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaoyu Wu,Zhi Peng,Ying Xu,Zixuan Sheng,Yanshan Liu,Youguo Liao,Yin Wang,Ya Wen,Junzhi Yi,Chang Xie,Xuri Chen,Jiajie Hu,Bingqian Yan,Huijing Wang,Xudong Yao,Wei Fu,Hongwei OuyangPubMed 35033199
- Annals of translational medicine 9:13762021TNF-α-stimulated nucleus pulposus cells induce cell apoptosis through the release of exosomal miR-16 targeting IGF-1 and IGF-1R in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi-Chen Zhang,Yan-Pei Zou,Shun-Qi Hu,Tai-Wei Zhang,Hao Zhou,Bing Liang,Chen-Yang Zhuang,Hui-Ren Wang,Li-Bo Jiang,Xi-Lei LiPubMed 34733928
- Frontiers in pharmacology 12:6243032021Yi-Qi-Jian-Pi-Xiao-Yu-Xie-Zhuo Formula Improves Muscle Atrophy via Modulating the IGF-1/PI3K/Akt Signaling Pathway in 5/6 Nephrectomized Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong Xia,Bingbing Zhang,Dan Yang,Chengyue Zhu,Jiudan Zhang,Hongbo Chen,Hongzhen Ma,Shouci Hu,Chao Xu,Chengqian Shi,Keda Lu,Peipei ZhangPubMed 33986663
- Diagnostic pathology 14:1062019LncRNA DNM3OS promotes proliferation and inhibits apoptosis through modulating IGF1 expression by sponging MiR-126 in CHON-001 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDi Ai et. al.PubMed 31526393
- Stem cell research & therapy 10:2702019Mesenchymal stem cells restore the sperm motility from testicular torsion-detorsion injury by regulation of glucose metabolism in sperm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChi-Hao Hsiao et. al.PubMed 31445515
- Frontiers in cellular neuroscience 13:2982019Astrocytic Insulin-Like Growth Factor-1 Protects Neurons Against Excitotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Chen et. al.PubMed 31338023
- International journal of molecular medicine 43:807-8202018Electro-acupuncture-induced neuroprotection is associated with activation of the IGF-1/PI3K/Akt pathway following adjacent dorsal root ganglionectomies in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Hu,Min-Nan Lu,Bo Chen,Jun Tong,Rui Mao,Shan-Shan Li,Pin Dai,Ya-Xin Tan,Yan-Bin XiyangPubMed 30569108
- Endocrinology 159:2337-23472018Luteinizing Hormone Facilitates Antral Follicular Maturation and Survival via Thecal Paracrine Signaling in Cattle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatsushige Hattori,Makoto Orisaka,Shin Fukuda,Kimihisa Tajima,Yukiko Yamazaki,Tetsuya Mizutani,Yoshio YoshidaPubMed 29668890
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human IGF1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link P05019
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Reactivity data
Select an application| ICC | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-2 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed:21076856, PubMed:24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:19578119, PubMed:22351760, PubMed:23243309, PubMed:23696648). As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).
Alternative names
IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF
Recommended products
Goat Polyclonal IGF1 antibody. Suitable for ICC, WB, IHC-P and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human IGF1 aa 100-150.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human IGF1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link P05019
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The insulin-like growth factor 1 or IGF1 is a protein hormone that plays an important mechanical role in growth and development. Alternative names for IGF1 include somatomedin C. This protein has a mass of approximately 7.6 kDa and is mainly produced by the liver. IGF1 is expressed in a range of tissues including muscle bone and cartilage reflecting its importance in various physiological processes. Its production is stimulated by growth hormone and it acts in an autocrine and paracrine manner to exert its effects.
- Biological function summary
IGF1 stimulates growth and proliferation of cells most notably impacting the development of the skeletal system and the regulation of apoptosis. It is not part of a larger protein complex but it interacts closely with IGF1 receptor to execute its functions. By binding to this receptor IGF1 activates intracellular signaling cascades that promote anabolic effects which include increased uptake of glucose and amino acids contributing to muscle and tissue growth.
- Pathways
IGF1 operates within the PI3K-Akt and MAPK signaling pathways which are significant for cell survival and proliferation. Its interaction with proteins like IGF binding protein 3 (IGFBP3) modulates its availability and activity in the bloodstream. These pathways are critical for mediating the effects of growth hormone highlighting the role of IGF1 in systemic growth and metabolism regulation.
- Associated diseases and disorders
Alterations in IGF1 levels associate with growth abnormalities such as Laron syndrome and acromegaly. Laron syndrome results from the body's inability to use IGF1 properly due to receptor defects while acromegaly occurs from excessive IGF1 production often due to pituitary tumors. Furthermore aberrant IGF1 signaling connects to cancer development where it can drive tumor growth and metastasis. Drugs targeting IGF1 and its pathway components continue to be researched for therapeutic potential in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry - Anti-IGF1 antibody (ab106836)
Immunocytochemistry analysis of paraformaldehyde fixed HEK-293 (human epithelial cell line from embryonic kidney) cells, permeabilized with 0.15% Triton. Primary incubation for 1 hour at 10 μg/mL followed by Alexa Fluor® 488 secondary antibody at 2 μg/mL. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (2 μg/mL).
Western blot - Anti-IGF1 antibody (ab106836)
Primary incubation for 1 hour at room temperature. Detected by chemiluminescence.
All lanes: Western blot - Anti-IGF1 antibody (ab106836) at 0.5 µg/mL
Lane 1: Human uterus tissue lysate in RIPA buffer at 35 µg
Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate in RIPA buffer at 35 µg
Predicted band size: 22 kDa
Observed band size: 15 kDa
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