Rabbit Recombinant Monoclonal IGF1 antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Human samples.
Images
![Western blot - Anti-IGF1 antibody [EPR5099] - BSA and Azide free (AB221959), expandable thumbnail](https://content.abcam.com/products/images/igf1-antibody-epr5099-bsa-and-azide-free-ab221959--western-blot-img61337.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IGF1 antibody [EPR5099] - BSA and Azide free (AB221959)")
![Western blot - Anti-IGF1 antibody [EPR5099] - BSA and Azide free (AB221959), expandable thumbnail](https://content.abcam.com/products/images/igf1-antibody-epr5099-bsa-and-azide-free-ab221959--western-blot-img133898.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IGF1 antibody [EPR5099] - BSA and Azide free (AB221959)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Recombinant fragment - Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed:21076856, PubMed:24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:19578119, PubMed:22351760, PubMed:23243309, PubMed:23696648). As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).
Alternative names
IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF
Recommended products
Rabbit Recombinant Monoclonal IGF1 antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5099
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab221959 is the carrier-free version of Anti-IGF1 antibody [EPR5099] ab134140.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The insulin-like growth factor 1 or IGF1 is a protein hormone that plays an important mechanical role in growth and development. Alternative names for IGF1 include somatomedin C. This protein has a mass of approximately 7.6 kDa and is mainly produced by the liver. IGF1 is expressed in a range of tissues including muscle bone and cartilage reflecting its importance in various physiological processes. Its production is stimulated by growth hormone and it acts in an autocrine and paracrine manner to exert its effects.
- Biological function summary
IGF1 stimulates growth and proliferation of cells most notably impacting the development of the skeletal system and the regulation of apoptosis. It is not part of a larger protein complex but it interacts closely with IGF1 receptor to execute its functions. By binding to this receptor IGF1 activates intracellular signaling cascades that promote anabolic effects which include increased uptake of glucose and amino acids contributing to muscle and tissue growth.
- Pathways
IGF1 operates within the PI3K-Akt and MAPK signaling pathways which are significant for cell survival and proliferation. Its interaction with proteins like IGF binding protein 3 (IGFBP3) modulates its availability and activity in the bloodstream. These pathways are critical for mediating the effects of growth hormone highlighting the role of IGF1 in systemic growth and metabolism regulation.
- Associated diseases and disorders
Alterations in IGF1 levels associate with growth abnormalities such as Laron syndrome and acromegaly. Laron syndrome results from the body's inability to use IGF1 properly due to receptor defects while acromegaly occurs from excessive IGF1 production often due to pituitary tumors. Furthermore aberrant IGF1 signaling connects to cancer development where it can drive tumor growth and metastasis. Drugs targeting IGF1 and its pathway components continue to be researched for therapeutic potential in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-IGF1 antibody [EPR5099] - BSA and Azide free (ab221959), expandable thumbnail](https://content.abcam.com/products/images/igf1-antibody-epr5099-bsa-and-azide-free-ab221959--western-blot-img61337.jpg "Western blot - Anti-IGF1 antibody [EPR5099] - BSA and Azide free (ab221959)")
Western blot - Anti-IGF1 antibody [EPR5099] - BSA and Azide free (ab221959)
This data was developed using Anti-IGF1 antibody [EPR5099] ab134140, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-IGF1 antibody [EPR5099] (Anti-IGF1 antibody [EPR5099] ab134140) at 1/20000 dilution
All lanes: Human IGF-1 recombinant protein at 10 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 10 kDa
![Western blot - Anti-IGF1 antibody [EPR5099] - BSA and Azide free (ab221959), expandable thumbnail](https://content.abcam.com/products/images/igf1-antibody-epr5099-bsa-and-azide-free-ab221959--western-blot-img133898.jpg "Western blot - Anti-IGF1 antibody [EPR5099] - BSA and Azide free (ab221959)")
Western blot - Anti-IGF1 antibody [EPR5099] - BSA and Azide free (ab221959)
This data was developed using Anti-IGF1 antibody [EPR5099] ab134140, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-IGF1 antibody [EPR5099] (Anti-IGF1 antibody [EPR5099] ab134140) at 1/10000 dilution
All lanes: IGF1 recombinant protein at 0.005 µg
SecondaryAll lanes: Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 22 kDa
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