Rabbit Recombinant Monoclonal IGF1 antibody. Carrier free. Suitable for ELISA, WB and reacts with Human, Recombinant fragment - Human samples.
Images
![Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (AB221958), expandable thumbnail](https://content.abcam.com/products/images/igf1-igf2-antibody-epr50982-bsa-and-azide-free-ab221958--western-blot-img81969.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (AB221958)")
![ELISA - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (AB221958), expandable thumbnail](https://content.abcam.com/products/images/igf1-igf2-antibody-epr50982-bsa-and-azide-free-ab221958--elisa-img83575.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (AB221958)")
![OI-RD Scanning - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (AB221958), expandable thumbnail](https://content.abcam.com/products/images/igf1-igf2-antibody-epr50982-bsa-and-azide-free-ab221958--oi-rd-scanning-img97833.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (AB221958)")
![Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (AB221958), expandable thumbnail](https://content.abcam.com/products/images/igf1-igf2-antibody-epr50982-bsa-and-azide-free-ab221958--western-blot-img436286.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (AB221958)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ELISA | WB | IP | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Expected | Not recommended | Not recommended |
| Recombinant fragment - Human | Not recommended | Tested | Not recommended | Not recommended |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes Tested with recombinant protein. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
Target data
Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed:21076856, PubMed:24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:19578119, PubMed:22351760, PubMed:23696648, PubMed:23243309).
Alternative names
Insulin-like growth factor I, IGF-I, Mechano growth factor, Somatomedin-C, MGF, IGF1, IBP1
Recommended products
Rabbit Recombinant Monoclonal IGF1 antibody. Carrier free. Suitable for ELISA, WB and reacts with Human, Recombinant fragment - Human samples.
Alternative names
Insulin-like growth factor I, IGF-I, Mechano growth factor, Somatomedin-C, MGF, IGF1, IBP1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR5098(2)
- Purification technique
Affinity purification Protein A
- Dissociation constant
4.2 x 10-11 M
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab221958 is the carrier-free version of Anti-IGF1 + IGF2 antibody [EPR5098(2)] ab133542.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (ab221958), expandable thumbnail](https://content.abcam.com/products/images/igf1-igf2-antibody-epr50982-bsa-and-azide-free-ab221958--western-blot-img81969.jpg "Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (ab221958)")
Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (ab221958)
This data was developed using Anti-IGF1 + IGF2 antibody [EPR5098(2)] ab133542, the same antibody clone in a different buffer formulation.
The recombinant IGF-I fragment corresponds to amino acids 49-118 of human IGF-1 with the molecular weight of 10kDa.
All lanes: Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] (Anti-IGF1 + IGF2 antibody [EPR5098(2)] ab133542) at 1/1000 dilution
All lanes: Recombinant human IGF-I fragment at 0.005 µg
Predicted band size: 22 kDa
![ELISA - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (ab221958), expandable thumbnail](https://content.abcam.com/products/images/igf1-igf2-antibody-epr50982-bsa-and-azide-free-ab221958--elisa-img83575.jpg "ELISA - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (ab221958)")
ELISA - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (ab221958)
This data was developed using Anti-IGF1 + IGF2 antibody [EPR5098(2)] ab133542, the same antibody clone in a different buffer formulation.Primary antibody, ab33542 was used at concentrations between 0.1 - 1000ng/ml. An Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at 1:2500 dilution. Antigen concentration was 1000ng/ml.
![OI-RD Scanning - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (ab221958), expandable thumbnail](https://content.abcam.com/products/images/igf1-igf2-antibody-epr50982-bsa-and-azide-free-ab221958--oi-rd-scanning-img97833.jpg "OI-RD Scanning - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (ab221958)")
OI-RD Scanning - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (ab221958)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.![Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (ab221958), expandable thumbnail](https://content.abcam.com/products/images/igf1-igf2-antibody-epr50982-bsa-and-azide-free-ab221958--western-blot-img436286.jpg "Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (ab221958)")
Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] - BSA and Azide free (ab221958)
This data was developed using Anti-IGF1 + IGF2 antibody [EPR5098(2)] ab133542, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM /TBST
Predicted band size of IGF1 and IGF2 should be 22kDa, both Anti-IGF1 + IGF2 antibody [EPR5098(2)] ab133542 and anti-His tag antibody detected dimer at 44kDa.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control at 1/1000000 dilution.
All lanes: Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] (Anti-IGF1 + IGF2 antibody [EPR5098(2)] ab133542) at 1/1000 dilution
Lane 1: HEK-293T transfected with an empty vector whole cell lysate at 20 µg
Lane 2: HEK-293T transfected with a human IGF1 expression vector containing a His tag whole cell lysate at 20 µg
Lane 3: HEK-293T transfected with a human IGF2 expression vector containing a His tag whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 44 kDa
Exposure time: 3s
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